FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023270997> ?p ?o ?g. }

• W2023270997 endingPage "17544" @default.
• W2023270997 startingPage "17537" @default.
• W2023270997 abstract "To elucidate the energetic features of the anomalously high-level stabilization of a hyperthermophile pyrrolidone carboxyl peptidase (PfPCP) from a hyperthermophilic archaeon, Pyrococcus furiosus, equilibrium and kinetic studies of the guanidine hydrochloride (GuHCl)-induced unfolding and refolding were carried out with CD measurements at 220 nm in comparison with those from the mesophile homologue (BaPCP) from Bacillus amyloliquefaciens. The mutant protein of PfPCP substituted with Ser at both Cys142 and Cys188 (PfC142/188S) was used. The GuHCl unfolding for PfC142/188S and BaPCP was reversible. It was difficult to obtain the equilibrated unfolding curve of the hyperthermophile proteins at temperatures below 50 degreesC and pH 7, because of the remarkably slow rate of the unfolding. The unfolding for PfC142/188S attained equilibrium after 7 days at 60 degreesC, resulting in the coincidence between the unfolding and refolding curves. The Gibbs energy change of unfolding, DeltaGH2O (56.6 kJ/mol), for PfC142/188S at 60 degreesC and pH 7 was dramatically higher than that (7.6 kJ/mol) for BaPCP at 40 degreesC and pH 7. The unfolding and refolding kinetics for PfC142/188S and BaPCP at both 25 and 60 degreesC at pH 7 were approximated as a single exponential. The rate constant in water (kuH2O) of the unfolding reaction for PfC142/188S (1.6 x 10(-)15 s-1) at 25 degreesC and pH 7 was drastically reduced by 7 orders of magnitude compared to that (1.5 x 10(-)8 s-1) for BaPCP, whereas the refolding rates (krH2O) in water for PfC142/188S (9.3 x 10(-)2 s-1) and BaPCP (3.6 x 10(-)1 s-1) at 25 degreesC and pH 7 were similar. These results indicate that the greater stability of the hyperthermophile PCP was characterized by the drastically slow unfolding rate." @default.
• W2023270997 created "2016-06-24" @default.
• W2023270997 creator A5028649351 @default.
• W2023270997 creator A5035271517 @default.
• W2023270997 creator A5041279842 @default.
• W2023270997 creator A5053042729 @default.
• W2023270997 creator A5056614311 @default.
• W2023270997 creator A5062973607 @default.
• W2023270997 creator A5086114657 @default.
• W2023270997 date "1998-11-21" @default.
• W2023270997 modified "2023-09-26" @default.
• W2023270997 title "The Unusually Slow Unfolding Rate Causes the High Stability of Pyrrolidone Carboxyl Peptidase from a Hyperthermophile, <i>Pyrococcus furiosus</i>: Equilibrium and Kinetic Studies of Guanidine Hydrochloride-Induced Unfolding and Refolding" @default.
• W2023270997 cites W1516388695 @default.
• W2023270997 cites W1529551630 @default.
• W2023270997 cites W1573631659 @default.
• W2023270997 cites W1975055907 @default.
• W2023270997 cites W1984212866 @default.
• W2023270997 cites W1984326208 @default.
• W2023270997 cites W2001458612 @default.
• W2023270997 cites W2015279243 @default.
• W2023270997 cites W2035546546 @default.
• W2023270997 cites W2043174540 @default.
• W2023270997 cites W2059085556 @default.
• W2023270997 cites W2059575723 @default.
• W2023270997 cites W2082920672 @default.
• W2023270997 cites W2133698816 @default.
• W2023270997 cites W4243088827 @default.
• W2023270997 doi "https://doi.org/10.1021/bi9814585" @default.
• W2023270997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9860869" @default.
• W2023270997 hasPublicationYear "1998" @default.
• W2023270997 type Work @default.
• W2023270997 sameAs 2023270997 @default.
• W2023270997 citedByCount "47" @default.
• W2023270997 countsByYear W20232709972012 @default.
• W2023270997 countsByYear W20232709972013 @default.
• W2023270997 countsByYear W20232709972015 @default.
• W2023270997 countsByYear W20232709972016 @default.
• W2023270997 countsByYear W20232709972018 @default.
• W2023270997 countsByYear W20232709972020 @default.
• W2023270997 countsByYear W20232709972022 @default.
• W2023270997 crossrefType "journal-article" @default.
• W2023270997 hasAuthorship W2023270997A5028649351 @default.
• W2023270997 hasAuthorship W2023270997A5035271517 @default.
• W2023270997 hasAuthorship W2023270997A5041279842 @default.
• W2023270997 hasAuthorship W2023270997A5053042729 @default.
• W2023270997 hasAuthorship W2023270997A5056614311 @default.
• W2023270997 hasAuthorship W2023270997A5062973607 @default.
• W2023270997 hasAuthorship W2023270997A5086114657 @default.
• W2023270997 hasConcept C104317684 @default.
• W2023270997 hasConcept C121332964 @default.
• W2023270997 hasConcept C148898269 @default.
• W2023270997 hasConcept C178790620 @default.
• W2023270997 hasConcept C181199279 @default.
• W2023270997 hasConcept C185592680 @default.
• W2023270997 hasConcept C2776460385 @default.
• W2023270997 hasConcept C2776943354 @default.
• W2023270997 hasConcept C2779463914 @default.
• W2023270997 hasConcept C2781216036 @default.
• W2023270997 hasConcept C523546767 @default.
• W2023270997 hasConcept C54355233 @default.
• W2023270997 hasConcept C550995028 @default.
• W2023270997 hasConcept C55428944 @default.
• W2023270997 hasConcept C55493867 @default.
• W2023270997 hasConcept C62520636 @default.
• W2023270997 hasConcept C63223902 @default.
• W2023270997 hasConcept C8010536 @default.
• W2023270997 hasConcept C86803240 @default.
• W2023270997 hasConceptScore W2023270997C104317684 @default.
• W2023270997 hasConceptScore W2023270997C121332964 @default.
• W2023270997 hasConceptScore W2023270997C148898269 @default.
• W2023270997 hasConceptScore W2023270997C178790620 @default.
• W2023270997 hasConceptScore W2023270997C181199279 @default.
• W2023270997 hasConceptScore W2023270997C185592680 @default.
• W2023270997 hasConceptScore W2023270997C2776460385 @default.
• W2023270997 hasConceptScore W2023270997C2776943354 @default.
• W2023270997 hasConceptScore W2023270997C2779463914 @default.
• W2023270997 hasConceptScore W2023270997C2781216036 @default.
• W2023270997 hasConceptScore W2023270997C523546767 @default.
• W2023270997 hasConceptScore W2023270997C54355233 @default.
• W2023270997 hasConceptScore W2023270997C550995028 @default.
• W2023270997 hasConceptScore W2023270997C55428944 @default.
• W2023270997 hasConceptScore W2023270997C55493867 @default.
• W2023270997 hasConceptScore W2023270997C62520636 @default.
• W2023270997 hasConceptScore W2023270997C63223902 @default.
• W2023270997 hasConceptScore W2023270997C8010536 @default.
• W2023270997 hasConceptScore W2023270997C86803240 @default.
• W2023270997 hasIssue "50" @default.
• W2023270997 hasLocation W20232709971 @default.
• W2023270997 hasLocation W20232709972 @default.
• W2023270997 hasOpenAccess W2023270997 @default.
• W2023270997 hasPrimaryLocation W20232709971 @default.
• W2023270997 hasRelatedWork W1707430637 @default.
• W2023270997 hasRelatedWork W1975055907 @default.
• W2023270997 hasRelatedWork W1998041471 @default.
• W2023270997 hasRelatedWork W2015982116 @default.
• W2023270997 hasRelatedWork W2018983297 @default.
• W2023270997 hasRelatedWork W2023270997 @default.
• W2023270997 hasRelatedWork W2028779838 @default.

• next