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- W2023271072 abstract "Partial purification of topoisomerase II from small samples (10(7)-10(8) cells) of human leukaemic cells was achieved by isolation of cell nuclei, hyper-osmotic extraction of nuclear proteins, sorption of nuclear proteins by heparin-Sepharose and elution with potassium phosphate. Similar results were obtained by gradient and batchwise elution. The calatylic activity of topoisomerase increased ca. eightfold after removal of ca. 95% of the contaminating nuclear proteins. The conserved enzymatic activity after partial purification indicates that the enzyme was not damaged. The half-life of enzymatic activity is increased by the chromatographic procedure. Owing to its high yield and technical simplicity, this could be a candidate procedure for the study of topoisomerase II in patient-derived blood samples." @default.
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- W2023271072 date "1992-11-01" @default.
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- W2023271072 title "Activation of topoisomerase II during partial purification by heparin—Sepharose chromatography" @default.
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- W2023271072 doi "https://doi.org/10.1016/0021-9673(92)87222-t" @default.
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