Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023273485> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2023273485 endingPage "234" @default.
- W2023273485 startingPage "230" @default.
- W2023273485 abstract "A simple and specific method for analyzing thiols and disulfides on the basis of the reversibility of N-ethylmaleimide (NEM) alkylation of thiols is described. When the adduct of NEM and glutathione (GSH) was electrolyzed at neutral pH, all of the GSH was recovered. When the adduct was exposed to pH 11.0 for 15 min at 30 degrees C before electrolysis, GSH was not detected. The same behavior was observed after protein thiols reacted with NEM. This pH-dependent production of thiol from the adduct was used to assay GSH and oxidized glutathione in yeast cells, to assay sulfhydryl groups and disulfide bonds in authentic proteins, and to protect thiols from oxidation during enzymatic digestion of protein. This method is useful for assay of thiols and disulfides of both small and large molecules and can be used to identify labile thiols in biological samples that are oxidized during extraction procedures." @default.
- W2023273485 created "2016-06-24" @default.
- W2023273485 creator A5075164259 @default.
- W2023273485 creator A5091176815 @default.
- W2023273485 date "1992-02-01" @default.
- W2023273485 modified "2023-10-14" @default.
- W2023273485 title "Assay of thiols and disulfides based on the reversibility of N-ethylmaleimide alkylation of thiols combined with electrolysis" @default.
- W2023273485 cites W2009119064 @default.
- W2023273485 cites W2022546712 @default.
- W2023273485 cites W2047548269 @default.
- W2023273485 cites W2050408395 @default.
- W2023273485 cites W2314315518 @default.
- W2023273485 cites W2327305530 @default.
- W2023273485 cites W29716068 @default.
- W2023273485 cites W8750466 @default.
- W2023273485 doi "https://doi.org/10.1016/0003-2697(92)90457-i" @default.
- W2023273485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1632486" @default.
- W2023273485 hasPublicationYear "1992" @default.
- W2023273485 type Work @default.
- W2023273485 sameAs 2023273485 @default.
- W2023273485 citedByCount "17" @default.
- W2023273485 countsByYear W20232734852012 @default.
- W2023273485 countsByYear W20232734852013 @default.
- W2023273485 countsByYear W20232734852016 @default.
- W2023273485 countsByYear W20232734852019 @default.
- W2023273485 countsByYear W20232734852020 @default.
- W2023273485 countsByYear W20232734852022 @default.
- W2023273485 crossrefType "journal-article" @default.
- W2023273485 hasAuthorship W2023273485A5075164259 @default.
- W2023273485 hasAuthorship W2023273485A5091176815 @default.
- W2023273485 hasConcept C108204754 @default.
- W2023273485 hasConcept C161790260 @default.
- W2023273485 hasConcept C164361826 @default.
- W2023273485 hasConcept C178790620 @default.
- W2023273485 hasConcept C181199279 @default.
- W2023273485 hasConcept C185592680 @default.
- W2023273485 hasConcept C2777646807 @default.
- W2023273485 hasConcept C2779179908 @default.
- W2023273485 hasConcept C2779201268 @default.
- W2023273485 hasConcept C2781324293 @default.
- W2023273485 hasConcept C538909803 @default.
- W2023273485 hasConcept C55493867 @default.
- W2023273485 hasConceptScore W2023273485C108204754 @default.
- W2023273485 hasConceptScore W2023273485C161790260 @default.
- W2023273485 hasConceptScore W2023273485C164361826 @default.
- W2023273485 hasConceptScore W2023273485C178790620 @default.
- W2023273485 hasConceptScore W2023273485C181199279 @default.
- W2023273485 hasConceptScore W2023273485C185592680 @default.
- W2023273485 hasConceptScore W2023273485C2777646807 @default.
- W2023273485 hasConceptScore W2023273485C2779179908 @default.
- W2023273485 hasConceptScore W2023273485C2779201268 @default.
- W2023273485 hasConceptScore W2023273485C2781324293 @default.
- W2023273485 hasConceptScore W2023273485C538909803 @default.
- W2023273485 hasConceptScore W2023273485C55493867 @default.
- W2023273485 hasIssue "2" @default.
- W2023273485 hasLocation W20232734851 @default.
- W2023273485 hasLocation W20232734852 @default.
- W2023273485 hasOpenAccess W2023273485 @default.
- W2023273485 hasPrimaryLocation W20232734851 @default.
- W2023273485 hasRelatedWork W2008232785 @default.
- W2023273485 hasRelatedWork W2019296000 @default.
- W2023273485 hasRelatedWork W2023923217 @default.
- W2023273485 hasRelatedWork W2031480377 @default.
- W2023273485 hasRelatedWork W2038581871 @default.
- W2023273485 hasRelatedWork W2052955020 @default.
- W2023273485 hasRelatedWork W2071774543 @default.
- W2023273485 hasRelatedWork W2073709695 @default.
- W2023273485 hasRelatedWork W2163524858 @default.
- W2023273485 hasRelatedWork W2385716808 @default.
- W2023273485 hasVolume "200" @default.
- W2023273485 isParatext "false" @default.
- W2023273485 isRetracted "false" @default.
- W2023273485 magId "2023273485" @default.
- W2023273485 workType "article" @default.