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• W2023273560 abstract "Abstract TT virus‐like minivirus (TLMV) was recently discovered as a human circovirus. Little is known about its natural history and molecular epidemiology. A study of TLMV infection is described in a population of French hemodialysis patients. TLMV DNA was tested by seminested PCR system located in the noncoding region in 81 patients divided into seven groups according to the origin of their renal disease. Quantitation of TLMV DNA in serum was carried out. Sequences from 28 patients were compared with 40 sequences retrieved from databases and 53 TLMV sequences cloned from the serum of a single patient. The prevalence of TLMV DNA in hemodialysis patients was 95.1%. In this study, 24 samples (29.6%) presented viral loads of > 125 equivalents of plasmid (Ep)/ml, and only 6 (7.8%) had viral loads of > 125 × 10 2 Ep/ml. A significant correlation ( P < 0.029) was found between viral loads of > 125 × 10 2 Ep/ml and the neoplastic origin of end‐stage renal disease. Analysis of 53 sequences cloned from a single individual demonstrated high sequence variability, as shown by the genetic distance of 40.2%. This genetic distance is comparable to that between the most divergent sequences of TLMV reported to date (43.5%). These data suggest that TLMV viral load is possibly related to the level of immunocompetence of hemodialysis patients; the genetic diversity of TLMV is extremely high; and co‐infection by different strains is possible. J. Med. Virol. 67:630–635, 2002. © 2002 Wiley‐Liss, Inc." @default.
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• W2023273560 date "2002-07-02" @default.
• W2023273560 modified "2023-10-15" @default.
• W2023273560 title "High genetic diversity revealed by the study of TLMV infection in French hemodialysis patients" @default.
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• W2023273560 doi "https://doi.org/10.1002/jmv.10150" @default.
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