Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023276960> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2023276960 abstract "[Leu-B25]insulin is a low affinity insulin analog which does not increase the rate of dissociation of 125I-insulin from insulin receptors (i.e. does not display negative cooperativity). We have studied the characteristics of binding of this analog to IM-9 cultured lymphocytes, in order to determine the contribution of negative cooperativity to the curvilinear nature of Scatchard plots typical of insulin binding data. The affinity of [LeuB25]insulin for receptors was approximately 1% that of insulin, as determined by its ability to inhibit 125I-insulin binding. Monoiodinated preparations of insulin and of [LeuB25]insulin were produced, labeled in the tyrosine at position 14 of the A chain. These 125I-TyrA14-labeled species were used in all studies. Both native insulin and a serum containing antiinsulin receptor antibodies were equally potent at inhibiting binding of 125I-[LeuB25]insulin and 125I-native insulin, suggesting that they bind to the same population of receptors. Native insulin (100 ng/ml) increased the rate of dissociation of both 125I-insulin and 125I-[LeuB25]insulin. However, [LeuB25]insulin (2.5 micrograms/ml) did not increase the rates of dissociation of either 125I-insulin or 125I-[LeuB25]insulin (i.e. it did not display negative cooperativity). Competition curves and Scatchard plots were constructed using 125I-[LeuB25]insulin and unlabeled analog. Half-maximal inhibition of 125I-[LeuB25]insulin binding was seen at a [LeuB25]insulin concentration of approximately 500 ng/ml. More importantly, the Scatchard plot of these binding data was markedly curvilinear, as is typical of insulin binding data. In summary, a non-cooperative insulin analog, [LeuB25]insulin, yielded curvilinear Scatchard plots, indicating that negative cooperativity is not the full explanation of the curvilinear nature of Scatchard plots of insulin binding data. Therefore, an alternative explanation, such as the existence of heterogeneous receptor states, with differing affinities for the hormone, must contribute to the nonlinearity of these plots." @default.
- W2023276960 created "2016-06-24" @default.
- W2023276960 creator A5007060336 @default.
- W2023276960 creator A5041635820 @default.
- W2023276960 creator A5070540159 @default.
- W2023276960 creator A5073130137 @default.
- W2023276960 creator A5091832115 @default.
- W2023276960 date "1983-12-01" @default.
- W2023276960 modified "2023-09-26" @default.
- W2023276960 title "Characteristics of Binding of a Low Affinity, Noncooperative Insulin ([LeuB25]Insulin) to IM-9 Lymphocytes*" @default.
- W2023276960 cites W1540487569 @default.
- W2023276960 cites W1573534810 @default.
- W2023276960 cites W175424067 @default.
- W2023276960 cites W1983649348 @default.
- W2023276960 cites W1984909981 @default.
- W2023276960 cites W2011249780 @default.
- W2023276960 cites W2039734922 @default.
- W2023276960 cites W2052922723 @default.
- W2023276960 cites W2074975964 @default.
- W2023276960 cites W2084804108 @default.
- W2023276960 cites W2736815058 @default.
- W2023276960 doi "https://doi.org/10.1210/endo-113-6-1963" @default.
- W2023276960 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6357764" @default.
- W2023276960 hasPublicationYear "1983" @default.
- W2023276960 type Work @default.
- W2023276960 sameAs 2023276960 @default.
- W2023276960 citedByCount "2" @default.
- W2023276960 crossrefType "journal-article" @default.
- W2023276960 hasAuthorship W2023276960A5007060336 @default.
- W2023276960 hasAuthorship W2023276960A5041635820 @default.
- W2023276960 hasAuthorship W2023276960A5070540159 @default.
- W2023276960 hasAuthorship W2023276960A5073130137 @default.
- W2023276960 hasAuthorship W2023276960A5091832115 @default.
- W2023276960 hasConcept C107824862 @default.
- W2023276960 hasConcept C112446052 @default.
- W2023276960 hasConcept C126322002 @default.
- W2023276960 hasConcept C134018914 @default.
- W2023276960 hasConcept C170493617 @default.
- W2023276960 hasConcept C185592680 @default.
- W2023276960 hasConcept C187250156 @default.
- W2023276960 hasConcept C2776664568 @default.
- W2023276960 hasConcept C2777391703 @default.
- W2023276960 hasConcept C2779306644 @default.
- W2023276960 hasConcept C3019948211 @default.
- W2023276960 hasConcept C55493867 @default.
- W2023276960 hasConcept C71924100 @default.
- W2023276960 hasConcept C86803240 @default.
- W2023276960 hasConceptScore W2023276960C107824862 @default.
- W2023276960 hasConceptScore W2023276960C112446052 @default.
- W2023276960 hasConceptScore W2023276960C126322002 @default.
- W2023276960 hasConceptScore W2023276960C134018914 @default.
- W2023276960 hasConceptScore W2023276960C170493617 @default.
- W2023276960 hasConceptScore W2023276960C185592680 @default.
- W2023276960 hasConceptScore W2023276960C187250156 @default.
- W2023276960 hasConceptScore W2023276960C2776664568 @default.
- W2023276960 hasConceptScore W2023276960C2777391703 @default.
- W2023276960 hasConceptScore W2023276960C2779306644 @default.
- W2023276960 hasConceptScore W2023276960C3019948211 @default.
- W2023276960 hasConceptScore W2023276960C55493867 @default.
- W2023276960 hasConceptScore W2023276960C71924100 @default.
- W2023276960 hasConceptScore W2023276960C86803240 @default.
- W2023276960 hasLocation W20232769601 @default.
- W2023276960 hasLocation W20232769602 @default.
- W2023276960 hasOpenAccess W2023276960 @default.
- W2023276960 hasPrimaryLocation W20232769601 @default.
- W2023276960 hasRelatedWork W1977031591 @default.
- W2023276960 hasRelatedWork W1992667308 @default.
- W2023276960 hasRelatedWork W1995368038 @default.
- W2023276960 hasRelatedWork W2018280313 @default.
- W2023276960 hasRelatedWork W2020167192 @default.
- W2023276960 hasRelatedWork W2024261389 @default.
- W2023276960 hasRelatedWork W2024436229 @default.
- W2023276960 hasRelatedWork W2035407557 @default.
- W2023276960 hasRelatedWork W2093576471 @default.
- W2023276960 hasRelatedWork W2566765504 @default.
- W2023276960 isParatext "false" @default.
- W2023276960 isRetracted "false" @default.
- W2023276960 magId "2023276960" @default.
- W2023276960 workType "article" @default.