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- W2023277402 abstract "Abstract The number and concentration of electrophoretically distinguishable components in sap from uninfected tobacco leaves varied with the position of the leaf on the stem, the age of the plants, their nutrition and conditions of growth. None of the distinguishable proteins seemed to be homogeneous. Sap from the lowest leaves contained something that aggregated the proteins and produced unstable electrophoretic patterns; stable patterns were obtainable when the proteins were precipitated with ammonium sulfate and redissolved. Infection with tobacco mosaic virus or potato virus X, but not with tobacco etch or potato Y virus, introduced new components into the electrophoretic pattern. Unlike tobacco mosaic virus, potato virus X was stationary in pH 7 phosphate buffer; two minor components specific to sap from leaves infected with potato virus X had mobilities of −2 and −4 × 10 −5 cm 2 sec. −1 v −1 . The concentration of normal proteins was not correlated with severity of leaf symptoms, and was not consistently affected by virus infection. Potato Y and tobacco etch viruses usually did not affect the concentration, but sometimes decreased it slightly. Potato virus X and tobacco mosaic virus sometimes considerably decreased the concentration of normal proteins, usually when young rapidly growing plants were used and the conditions favored a high virus content, but even in these conditions the amount of normal proteins was sometimes unaffected. Simultaneous infection with potato X and tobacco mosaic viruses, each of which accumulated to the same extent as when present alone, had no more effect on the normal proteins than did infection with one or the other alone." @default.
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- W2023277402 date "1957-08-01" @default.
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- W2023277402 title "An electrophoretic study of sap from uninfected and virus-infected tobacco plants" @default.
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- W2023277402 doi "https://doi.org/10.1016/0042-6822(57)90041-7" @default.
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