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- W2023280070 abstract "Two mechanisms controlling stable RNA synthesis have been described: growth rate-dependent control and stringent response. Although the mechanism underlying growth rate-dependent control is still a matter of dispute, this control is commonly assumed to operate through repression of transcription initiation of stable RNA operons. The same is true for the stringent response. Here we show that the cell utilizes an additional control system operating through activation of the thrU(tufB) operon. This operon, the tyrT and the rrnB operon share a common trans-activating protein that binds to cis-acting DNA regions upstream of the promoters of the two tRNA operons and of the P1 promoter of the rrnB operon. Conceivably, more stable RNA operons may be regulated by trans-activation. Both in vivo and in vitro experiments show that the Escherichia coli protein FIS (Factor for Inversion Stimulation) is involved in the trans-activation. This protein is known to stimulate the inversion of various DNA segments by binding to cis-acting recombinational enhancers and functions as a host factor for the bacteriophages Mu and Lambda." @default.
- W2023280070 created "2016-06-24" @default.
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- W2023280070 date "1990-08-01" @default.
- W2023280070 modified "2023-09-26" @default.
- W2023280070 title "FIS-dependent trans-activation of tRNA and rRNA operons of Escherichia coli" @default.
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- W2023280070 doi "https://doi.org/10.1016/0167-4781(90)90184-4" @default.
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