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• W2023284867 abstract "The small G-protein Sar1 and the cytosolic complexes Sec23/24 and Sec13/31 associate sequentially on endoplasmic reticulum membranes to form a protein coat named COPII, which drives the formation of transport vesicles. Using dynamic light scattering, we show that Sec23/24 and Sec13/31 can self-assemble in a stoichiometric manner in solution to form particles with hydrodynamic radii in the range of 40-60 nm. Self-assembly is favoured by lowering the pH, the ionic strength and/or the temperature. Electron microscopy reveals the formation of spherical particles 60-120 nm in diameter with a tight, rough mesh on their surfaces. We suggest that these structures, which represent a minimal COPII cage, mimic the molecular organization of the membrane-associated COPII coat." @default.
• W2023284867 created "2016-06-24" @default.
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• W2023284867 date "2003-04-01" @default.
• W2023284867 modified "2023-10-08" @default.
• W2023284867 title "Self‐assembly of minimal COPII cages" @default.
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• W2023284867 doi "https://doi.org/10.1038/sj.embor.embor812" @default.
• W2023284867 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1319167" @default.
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