FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023289249> ?p ?o ?g. }

• W2023289249 endingPage "746" @default.
• W2023289249 startingPage "741" @default.
• W2023289249 abstract "Two genomic libraries from Acidaminococcus fermentans DNA constructed with the λ vectors gt11 and EMBL 3 were screened with antisera raised against 2-hydroxyglutaryl-CoA dehydratase. Two clones giving the strongest reaction in the immunoassay were analyzed further, one was a λgt11 clone with an insert of 2050 bp and one was a λEMBL-3 clone with an insert of approximately 11000 bp. Escherichia coli cells infected with the λgt11 clone expressed the α subunit of the dehydratase (Mr, 53870), whereas with the λEMBL-3 clone, the α and β subunits (Mr, 41857) were detected on Western blots. Restriction fragments of both clones were subcloned in pUC 8 and sequenced by the chain termination method. Thus the complete sequence of the genes of both subunits, hgdA (α) and hgdB (β) were obtained. The genes have the following order: A-B, with an intergenic region of only 2 bp. The deduced amino acid sequences for the α and β subunits were confirmed by four peptides sequenced by protein chemical methods. Both chains are extremely rich in cysteine (13 in α, including a CNC and two CC clusters, and nine in (β) but no similarities to other known protein sequences were found." @default.
• W2023289249 created "2016-06-24" @default.
• W2023289249 creator A5024196097 @default.
• W2023289249 creator A5045540122 @default.
• W2023289249 creator A5065831250 @default.
• W2023289249 creator A5084332430 @default.
• W2023289249 date "1989-05-01" @default.
• W2023289249 modified "2023-10-06" @default.
• W2023289249 title "Cloning and sequencing of the genes of 2-hydoxyglutaryl-CoA dehydratase from Acidaminococcus fermentans" @default.
• W2023289249 cites W1604889681 @default.
• W2023289249 cites W1968351818 @default.
• W2023289249 cites W1975304761 @default.
• W2023289249 cites W1994176891 @default.
• W2023289249 cites W2012847227 @default.
• W2023289249 cites W2027807422 @default.
• W2023289249 cites W2028954926 @default.
• W2023289249 cites W2032343668 @default.
• W2023289249 cites W2038124483 @default.
• W2023289249 cites W2049132034 @default.
• W2023289249 cites W2057616449 @default.
• W2023289249 cites W2074369584 @default.
• W2023289249 cites W2076076532 @default.
• W2023289249 cites W2100837269 @default.
• W2023289249 cites W2114624600 @default.
• W2023289249 cites W2138270253 @default.
• W2023289249 cites W2161524167 @default.
• W2023289249 cites W2166064969 @default.
• W2023289249 cites W2894366186 @default.
• W2023289249 cites W4231759100 @default.
• W2023289249 cites W4242292797 @default.
• W2023289249 doi "https://doi.org/10.1111/j.1432-1033.1989.tb14786.x" @default.
• W2023289249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2659350" @default.
• W2023289249 hasPublicationYear "1989" @default.
• W2023289249 type Work @default.
• W2023289249 sameAs 2023289249 @default.
• W2023289249 citedByCount "22" @default.
• W2023289249 countsByYear W20232892492012 @default.
• W2023289249 crossrefType "journal-article" @default.
• W2023289249 hasAuthorship W2023289249A5024196097 @default.
• W2023289249 hasAuthorship W2023289249A5045540122 @default.
• W2023289249 hasAuthorship W2023289249A5065831250 @default.
• W2023289249 hasAuthorship W2023289249A5084332430 @default.
• W2023289249 hasConcept C104317684 @default.
• W2023289249 hasConcept C121050878 @default.
• W2023289249 hasConcept C127413603 @default.
• W2023289249 hasConcept C128319531 @default.
• W2023289249 hasConcept C153911025 @default.
• W2023289249 hasConcept C159654299 @default.
• W2023289249 hasConcept C167625842 @default.
• W2023289249 hasConcept C170673058 @default.
• W2023289249 hasConcept C17757408 @default.
• W2023289249 hasConcept C19924922 @default.
• W2023289249 hasConcept C199360897 @default.
• W2023289249 hasConcept C2780098074 @default.
• W2023289249 hasConcept C2781374117 @default.
• W2023289249 hasConcept C41008148 @default.
• W2023289249 hasConcept C54355233 @default.
• W2023289249 hasConcept C78519656 @default.
• W2023289249 hasConcept C81089528 @default.
• W2023289249 hasConcept C84148353 @default.
• W2023289249 hasConcept C86803240 @default.
• W2023289249 hasConcept C91523082 @default.
• W2023289249 hasConceptScore W2023289249C104317684 @default.
• W2023289249 hasConceptScore W2023289249C121050878 @default.
• W2023289249 hasConceptScore W2023289249C127413603 @default.
• W2023289249 hasConceptScore W2023289249C128319531 @default.
• W2023289249 hasConceptScore W2023289249C153911025 @default.
• W2023289249 hasConceptScore W2023289249C159654299 @default.
• W2023289249 hasConceptScore W2023289249C167625842 @default.
• W2023289249 hasConceptScore W2023289249C170673058 @default.
• W2023289249 hasConceptScore W2023289249C17757408 @default.
• W2023289249 hasConceptScore W2023289249C19924922 @default.
• W2023289249 hasConceptScore W2023289249C199360897 @default.
• W2023289249 hasConceptScore W2023289249C2780098074 @default.
• W2023289249 hasConceptScore W2023289249C2781374117 @default.
• W2023289249 hasConceptScore W2023289249C41008148 @default.
• W2023289249 hasConceptScore W2023289249C54355233 @default.
• W2023289249 hasConceptScore W2023289249C78519656 @default.
• W2023289249 hasConceptScore W2023289249C81089528 @default.
• W2023289249 hasConceptScore W2023289249C84148353 @default.
• W2023289249 hasConceptScore W2023289249C86803240 @default.
• W2023289249 hasConceptScore W2023289249C91523082 @default.
• W2023289249 hasIssue "3" @default.
• W2023289249 hasLocation W20232892491 @default.
• W2023289249 hasLocation W20232892492 @default.
• W2023289249 hasOpenAccess W2023289249 @default.
• W2023289249 hasPrimaryLocation W20232892491 @default.
• W2023289249 hasRelatedWork W1998301160 @default.
• W2023289249 hasRelatedWork W2079797140 @default.
• W2023289249 hasRelatedWork W2140946425 @default.
• W2023289249 hasRelatedWork W2145368198 @default.
• W2023289249 hasRelatedWork W2177042266 @default.
• W2023289249 hasRelatedWork W2341696882 @default.
• W2023289249 hasRelatedWork W2376603524 @default.
• W2023289249 hasRelatedWork W2974345028 @default.
• W2023289249 hasRelatedWork W2980832024 @default.
• W2023289249 hasRelatedWork W3089331154 @default.
• W2023289249 hasVolume "181" @default.

• next