FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023289368> ?p ?o ?g. }

• W2023289368 endingPage "5178" @default.
• W2023289368 startingPage "5172" @default.
• W2023289368 abstract "Two 5'-modified (2'-5')(A)4 oligomers with an increased resistance to phosphatase degradation were synthesized and evaluated for their ability to develop an antiviral response when introduced into intact cells by microinjection or by chemical conjugation to poly(L-lysine). The enzymatic synthesis of 5'-gamma-phosphorothioate and beta,gamma-difluoromethylene (2'-5')(A)4 from adenosine 5'-O-(3-thiotriphosphate) and adenosine beta,gamma-difluoromethylenetriphosphate by (2'-5')-oligoadenylate synthetase is described. The isolation and characterization of these (2'-5')(A)4 analogues were achieved by high-performance liquid chromatography. The structures of 5'-modified tetramers were corroborated by enzyme digestion. These two 5'-modified tetramers compete as efficiently as natural (2'-5')(A)4 for the binding of a radiolabeled (2'-5')(A)4 probe to ribonuclease (RNase) L. Nevertheless, at the opposite to 5'-gamma-phosphorothioate (2'-5')(A)4, beta,gamma-difluoromethylene (2'-5')(A)4 failed to induce an antiviral response after microinjection in HeLa cells. In addition, it behaves as an antagonist of RNase L as demonstrated by its ability to inhibit the antiviral properties of 5'-gamma-phosphorothioate (2'-5')(A)4 when both are microinjected in HeLa cells. The increased metabolic stability of 5'-gamma-phosphorothioate (2'-5')(A)4 as compared to that of (2'-5')(A)4 was first demonstrated in cell-free extracts and then confirmed in intact cells after introduction in the form of a conjugate to poly(L-lysine). Indeed, 5'-gamma-phosphorothioate (2'-5')(A)4-poly(L-lysine) conjugate induces protein synthesis inhibition and characteristic ribosomal RNA cleavages for longer times than unmodified (2'-5')(A)4-poly(L-lysine) in the same cell system.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W2023289368 created "2016-06-24" @default.
• W2023289368 creator A5003140918 @default.
• W2023289368 creator A5013019477 @default.
• W2023289368 creator A5022874714 @default.
• W2023289368 creator A5027968360 @default.
• W2023289368 creator A5069624232 @default.
• W2023289368 creator A5070492404 @default.
• W2023289368 creator A5073219925 @default.
• W2023289368 creator A5088704587 @default.
• W2023289368 date "1987-08-01" @default.
• W2023289368 modified "2023-10-16" @default.
• W2023289368 title "5'-Modified agonist and antagonist (2'-5')(A)n analogs: synthesis and biological activity" @default.
• W2023289368 cites W1416006092 @default.
• W2023289368 cites W1525552183 @default.
• W2023289368 cites W1561048695 @default.
• W2023289368 cites W166257218 @default.
• W2023289368 cites W1671413742 @default.
• W2023289368 cites W1775749144 @default.
• W2023289368 cites W1967936672 @default.
• W2023289368 cites W1968345770 @default.
• W2023289368 cites W1980545483 @default.
• W2023289368 cites W1996122827 @default.
• W2023289368 cites W2005473390 @default.
• W2023289368 cites W2006232009 @default.
• W2023289368 cites W2016749199 @default.
• W2023289368 cites W2026832246 @default.
• W2023289368 cites W2030131332 @default.
• W2023289368 cites W2035488179 @default.
• W2023289368 cites W2045344712 @default.
• W2023289368 cites W2048940551 @default.
• W2023289368 cites W2054546881 @default.
• W2023289368 cites W2076300996 @default.
• W2023289368 cites W2079749769 @default.
• W2023289368 cites W2083289145 @default.
• W2023289368 cites W2094150819 @default.
• W2023289368 cites W2100837269 @default.
• W2023289368 cites W2132641862 @default.
• W2023289368 cites W2221020875 @default.
• W2023289368 cites W2404877807 @default.
• W2023289368 doi "https://doi.org/10.1021/bi00390a041" @default.
• W2023289368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3117112" @default.
• W2023289368 hasPublicationYear "1987" @default.
• W2023289368 type Work @default.
• W2023289368 sameAs 2023289368 @default.
• W2023289368 citedByCount "27" @default.
• W2023289368 countsByYear W20232893682012 @default.
• W2023289368 countsByYear W20232893682014 @default.
• W2023289368 countsByYear W20232893682017 @default.
• W2023289368 crossrefType "journal-article" @default.
• W2023289368 hasAuthorship W2023289368A5003140918 @default.
• W2023289368 hasAuthorship W2023289368A5013019477 @default.
• W2023289368 hasAuthorship W2023289368A5022874714 @default.
• W2023289368 hasAuthorship W2023289368A5027968360 @default.
• W2023289368 hasAuthorship W2023289368A5069624232 @default.
• W2023289368 hasAuthorship W2023289368A5070492404 @default.
• W2023289368 hasAuthorship W2023289368A5073219925 @default.
• W2023289368 hasAuthorship W2023289368A5088704587 @default.
• W2023289368 hasConcept C104317684 @default.
• W2023289368 hasConcept C10879258 @default.
• W2023289368 hasConcept C134306372 @default.
• W2023289368 hasConcept C1491633281 @default.
• W2023289368 hasConcept C181199279 @default.
• W2023289368 hasConcept C185592680 @default.
• W2023289368 hasConcept C197336794 @default.
• W2023289368 hasConcept C2776016237 @default.
• W2023289368 hasConcept C2776813977 @default.
• W2023289368 hasConcept C2776991684 @default.
• W2023289368 hasConcept C2777366897 @default.
• W2023289368 hasConcept C2778976237 @default.
• W2023289368 hasConcept C33923547 @default.
• W2023289368 hasConcept C515207424 @default.
• W2023289368 hasConcept C55493867 @default.
• W2023289368 hasConcept C67705224 @default.
• W2023289368 hasConcept C71240020 @default.
• W2023289368 hasConceptScore W2023289368C104317684 @default.
• W2023289368 hasConceptScore W2023289368C10879258 @default.
• W2023289368 hasConceptScore W2023289368C134306372 @default.
• W2023289368 hasConceptScore W2023289368C1491633281 @default.
• W2023289368 hasConceptScore W2023289368C181199279 @default.
• W2023289368 hasConceptScore W2023289368C185592680 @default.
• W2023289368 hasConceptScore W2023289368C197336794 @default.
• W2023289368 hasConceptScore W2023289368C2776016237 @default.
• W2023289368 hasConceptScore W2023289368C2776813977 @default.
• W2023289368 hasConceptScore W2023289368C2776991684 @default.
• W2023289368 hasConceptScore W2023289368C2777366897 @default.
• W2023289368 hasConceptScore W2023289368C2778976237 @default.
• W2023289368 hasConceptScore W2023289368C33923547 @default.
• W2023289368 hasConceptScore W2023289368C515207424 @default.
• W2023289368 hasConceptScore W2023289368C55493867 @default.
• W2023289368 hasConceptScore W2023289368C67705224 @default.
• W2023289368 hasConceptScore W2023289368C71240020 @default.
• W2023289368 hasIssue "16" @default.
• W2023289368 hasLocation W20232893681 @default.
• W2023289368 hasLocation W20232893682 @default.
• W2023289368 hasOpenAccess W2023289368 @default.
• W2023289368 hasPrimaryLocation W20232893681 @default.
• W2023289368 hasRelatedWork W1484857201 @default.

• next