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• W2023289507 abstract "The RNA of an established line of Chinese hamster cells growing in cell culture contains a small number of uridines substituted at the 3-position with a γ-linked α-aminobutyrate residue. Structure has been ascertained by: (a) examination of incorporation of isotopic labels from precursors; (b) degradation with anhydrous hydrazine and comparison of the products with synthetic material or with hydrazinolysis products of known uridines; and (c) comparison of the unknowns as their hydantoin derivatives with the 5-β-(bromoethyl)hydantoin alkylation products of uridine and of 1- and 3-methylpseudouridine. In this manner it is shown that 18-S RNA of ribosomes contains a single residue of a nucleoside which we tentatively identify as 1-methyl-3-γ-(α-amino-α-carboxypropyl)pseudouridine per molecule. RNA isolated from the supernatant fraction, sedimenting at 4 S and co-electrophoresing with transfer RNA on polyacrylamide gels, contains several similar bases, one of which is identified as 3-γ-(α-amino-α-carboxypropyl)uridine. Each of the above nucleosides derives its α-aminobutyrate residue from methionine." @default.
• W2023289507 created "2016-06-24" @default.
• W2023289507 creator A5020335877 @default.
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• W2023289507 date "1974-04-01" @default.
• W2023289507 modified "2023-09-27" @default.
• W2023289507 title "The isolation from ribonucleic acid of substituted uridines containing α-aminobutyrate moieties derived from methionine" @default.
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• W2023289507 doi "https://doi.org/10.1016/0005-2787(74)90009-4" @default.
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