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• W2023292189 abstract "Binding of proteins to the plasma membrane can be achieved with various membrane targeting motifs, including combinations of fatty acids, isoprenoids, and basic domains. In this study, we investigate whether attachment of different membrane targeting motifs influences the signaling capacity of membrane-bound signal transduction proteins by directing the proteins to different membrane microdomains. We used c-Raf-1 as a model for a signaling protein that is activated when membrane-bound. Three different membrane targeting motifs from K-Ras, Fyn, and Src proteins were fused to the N or C terminus of Raf-1. The ability of the modified Rafs to initiate MAPK signaling was then investigated. All three modified Raf-1 constructs activated MAPK to nearly equivalent levels. The extent of localization of the Raf-1 constructs to membrane microdomains known as rafts did not correlate with the level of MAPK activation. Moreover, treatment of cells with the raft disrupting drug methyl-β-cyclodextrin (MβCD) caused activation of MAPK to levels equivalent to those achieved with membrane-targeted Raf constructs. The use of pharmacological agents as well as dominant negative mutants revealed that MAPK activation by MβCD proceeds via a phosphoinositide 3-kinase-dependent mechanism that is Ras/Raf-independent. We conclude that cholesterol depletion from the plasma membrane by MβCD constitutes an alternative pathway for activating MAPK. Binding of proteins to the plasma membrane can be achieved with various membrane targeting motifs, including combinations of fatty acids, isoprenoids, and basic domains. In this study, we investigate whether attachment of different membrane targeting motifs influences the signaling capacity of membrane-bound signal transduction proteins by directing the proteins to different membrane microdomains. We used c-Raf-1 as a model for a signaling protein that is activated when membrane-bound. Three different membrane targeting motifs from K-Ras, Fyn, and Src proteins were fused to the N or C terminus of Raf-1. The ability of the modified Rafs to initiate MAPK signaling was then investigated. All three modified Raf-1 constructs activated MAPK to nearly equivalent levels. The extent of localization of the Raf-1 constructs to membrane microdomains known as rafts did not correlate with the level of MAPK activation. Moreover, treatment of cells with the raft disrupting drug methyl-β-cyclodextrin (MβCD) caused activation of MAPK to levels equivalent to those achieved with membrane-targeted Raf constructs. The use of pharmacological agents as well as dominant negative mutants revealed that MAPK activation by MβCD proceeds via a phosphoinositide 3-kinase-dependent mechanism that is Ras/Raf-independent. We conclude that cholesterol depletion from the plasma membrane by MβCD constitutes an alternative pathway for activating MAPK. mitogen-activated protein kinase extracellular-regulated protein kinase MAPK/ERK kinase phosphoinositide 3-kinase methyl-β-cyclodextrin phosphate-buffered saline polyacrylamide gel electrophoresis phospho-ERK Covalent modification by fatty acylation and prenylation is found on a wide variety of viral and cellular signaling proteins, including protein kinases, guanine nucleotide-binding proteins, transmembrane receptors, and viral structural proteins (1Resh M.D. Biochim. Biophys. Acta. 1999; 1451: 1-16Crossref PubMed Scopus (1066) Google Scholar, 2Casey P.J. Science. 1995; 268: 221-225Crossref PubMed Scopus (726) Google Scholar, 3Milligan G. Parenti M. Magee A.I. Trends Biochem. Sci. 1995; 20: 181-187Abstract Full Text PDF PubMed Scopus (283) Google Scholar). Binding of proteins to the inner face of the plasma membrane can be achieved with various membrane targeting motifs, including combinations of fatty acids, isoprenoids, and polybasic domains. For example, Src uses myristate with a polybasic stretch of amino acids at the N terminus to achieve membrane binding, whereas Fyn, a Src family member, uses a myristate + palmitate motif (4Alland L. Peseckis S.M. Atherton R.E. Berthiaume L. Resh M.D. J. Biol. Chem. 1994; 269: 16701-16705Abstract Full Text PDF PubMed Google Scholar, 5Sigal C.T. Zhou W. Buser C.A. McLaughlin S. Resh M.D. Proc. Natl. Acad. Sci. U. S. A. 1994; 91: 12253-12257Crossref PubMed Scopus (216) Google Scholar). A C-terminal farnesyl group in conjunction with a polybasic stretch (K-Ras) or palmitates (Ha-Ras) are required to promote membrane binding of Ras proteins (6Hancock J.F. Cadwallader K. Paterson H. Marshall C.J. EMBO J. 1991; 10: 4033-4039Crossref PubMed Scopus (374) Google Scholar, 7Cadwallader K.A. Paterson H. Macdonald S.G. Hancock J.F. Mol. Cell. Biol. 1994; 14: 4722-4730Crossref PubMed Scopus (151) Google Scholar). It is not clear why such a variety of targeting motifs exist and whether each individual motif promotes a unique function. Rafts are specialized plasma membrane microdomains that are highly enriched in cholesterol, glycosphingolipids, and glycophosphatidylinositol-anchored proteins (8Brown D.A. London E. Annu. Rev. Cell Dev. Biol. 1998; 14: 111-136Crossref PubMed Scopus (2530) Google Scholar, 9Brown D.A. London E. J. Biol. Chem. 2000; 275: 17221-17224Abstract Full Text Full Text PDF PubMed Scopus (2035) Google Scholar, 10Simons K. Ikonen E. Nature. 1997; 387: 569-572Crossref PubMed Scopus (7948) Google Scholar, 11Rodgers W. Crise B. Rose J.K. Mol. Cell. Biol. 1994; 14: 5384-5391Crossref PubMed Scopus (224) Google Scholar). Membrane rafts are relatively resistant to extraction with non-ionic detergents and exhibit a low buoyant density. Recent studies have shown that fatty acylation of proteins, such as Src family kinases and Gα subunits, promotes targeting to these membrane microdomains (12Robbins S.M. Quintrell N.A. Bishop J.M. Mol. Cell. Biol. 1995; 15: 3507-3515Crossref PubMed Scopus (227) Google Scholar, 13Shaul P.W. Smart E.J. Robinson L.J. German Z. Yuhanna I.S. Ying Y. Anderson R.G. Michel T. J. Biol. Chem. 1996; 271: 6518-6522Abstract Full Text Full Text PDF PubMed Scopus (621) Google Scholar, 14Shenoy-Scaria A.M. Dietzen D.J. Kwong J. Link D.C. Lublin D.M. J. Cell Biol. 1994; 126: 353-363Crossref PubMed Scopus (340) Google Scholar, 15van't Hof W. Resh M.D. J. Cell Biol. 1999; 145: 377-389Crossref PubMed Scopus (103) Google Scholar). Moreover, targeting of proteins to rafts is often required for the initiation of signal transduction. For example, fatty acylation and localization of Lck, Fyn, and LAT (linker for activation of T cells) to rafts are essential for their function in T cell receptor-mediated signal transduction (15van't Hof W. Resh M.D. J. Cell Biol. 1999; 145: 377-389Crossref PubMed Scopus (103) Google Scholar, 16Kabouridis P.S. Magee A.I. Ley S.C. EMBO J. 1997; 16: 4983-4998Crossref PubMed Scopus (315) Google Scholar, 17Zhang W. Trible R.P. Samelson L.E. Immunity. 1998; 9: 239-246Abstract Full Text Full Text PDF PubMed Scopus (747) Google Scholar). Other studies have shown that rafts link multiple receptor tyrosine kinases to mitogen-activated protein kinases (18Mineo C. James G.L. Smart E.J. Anderson R.G. J. Biol. Chem. 1996; 271: 11930-11935Abstract Full Text Full Text PDF PubMed Scopus (400) Google Scholar, 19Liu P. Ying Y. Ko Y.G. Anderson R.G. J. Biol. Chem. 1996; 271: 10299-10303Abstract Full Text Full Text PDF PubMed Scopus (336) Google Scholar). Furuchi and Anderson (20Furuchi T. Anderson R.G. J. Biol. Chem. 1998; 273: 21099-21104Abstract Full Text Full Text PDF PubMed Scopus (330) Google Scholar) also reported that cholesterol depletion of rafts/caveolae causes hyperactivation of MAPK.1 c-Raf-1, a serine/threonine protein kinase, plays a critical role in the mitogenic signal cascade. Activated Ras recruits cytosolic Raf to the inner surface of the plasma membrane where Raf is activated. It has been shown previously that Raf modified with the K-Ras membrane targeting motif is permanently targeted to the plasma membrane and is constitutively activated (21Leevers S.J. Paterson H.F. Marshall C.J. Nature. 1994; 369: 411-414Crossref PubMed Scopus (875) Google Scholar, 22Stokoe D. Macdonald S.G. Cadwallader K. Symons M. Hancock J.F. Science. 1994; 264: 1463-1467Crossref PubMed Scopus (832) Google Scholar). Here we used c-Raf-1 as a model to determine whether the attachment of different membrane-targeting motifs influences the signaling capacity of membrane-bound signaling molecules by directing the proteins to different membrane microdomains. The membrane-targeting motifs from Src, Fyn, and K-Ras were attached to c-Raf-1, and the ability of the modified Raf proteins to initiate MAPK signaling was investigated. All three modified Raf-1 constructs were targeted to the plasma membrane and activated MAPK to similar levels. However, the extent of raft localization did not correlate with the level of MAPK activation by each of the Raf constructs. Furthermore, we demonstrated that depletion of plasma membrane cholesterol by the raft disrupting drug methyl-β-cyclodextrin (MβCD) induced MAPK activation via a PI3K-dependent mechanism. These results suggest that the activity of key signaling proteins is regulated by plasma membrane cholesterol levels. Rabbit polyclonal anti-ERK2 (c-14), anti-caveolin-1 (N-20), and anti-Raf-1 (c-12) as well as monoclonal antic-Myc (9E10), anti-p-ERK (E-4), anti-phosphotyrosine (PY20), and anti-Ha-Ras were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Monoclonal anti-pan-Ras was from Calbiochem. Monoclonal anti-Raf-1 was purchased from Transduction Laboratories (Lexington, KY). Rabbit polyclonal anti-MAPK was purchased from New England Biolabs (Beverly, MA). The polyclonal PI3K antibody was purchased from Upstate Biotechnology Inc. (Lake Placid, NY). Fluorescein isothiocyanate-conjugated anti-mouse and rabbit secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). Optiprep was purchased from Life Technologies, Inc. Wortmannin, cholesterol, filipin, and methyl-β-cyclodextrin were purchased from Sigma. LY294002 was purchased from Biomol (Plymouth Meeting, PA). PD98059 was purchased from Calbiochem. Myc-Raf was constructed as follows. A sense primer was designed containing aSacI site, start codon, 10-amino acid Myc tag, and the N-terminal 6 amino acids of c-Raf. An antisense primer that began 661 bases from start codon of c-raf including a uniqueHindIII site was constructed. The polymerase chain reaction product generated from these two primers was digested withSacI and HindIII and used to replace the corresponding region of raf in pGEM-7Zf(+). For the Myc-Raf-K-Ras construct, an antisense primer was generated that encoded the 6 C-terminal amino acids of c-Raf-1, followed by the C-terminal 18 amino acids of K-Ras, a stop codon, and an XbaI site and three extra bases. A sense primer was constructed that included a unique EcoRV site upstream from the 3′ end of c-raf-1. The polymerase chain reaction product was digested with EcoRV/XbaI and subcloned into Myc-Raf-pGEM-7Zf(+) digested with the same enzymes. The construction of Raf-Myc was based on the same methods as those described for Myc-Raf-K-Ras, except the antisense primer contained codons for the 10-amino acid Myc tag. Fusion of the 16 N-terminal Fyn or 20 N-terminal Src amino acids onto the N terminus of c-Raf-1 was accomplished by the methods described for the Myc-Raf construction, except the sense primer encoded Fyn or Src amino acids. All constructs were verified by DNA sequencing prior to subcloning into the pCMV5 vector. Ha-RasN17 was a gift from Dr. Neal Rosen (Memorial Sloan-Kettering Cancer Center, New York). The Δp85 and p110-CAAXconstructs were gifts from Dr. Kurt Ballmer-Hofer (Paul Scherrer Institute, Switzerland). COS-1 cells were maintained as described previously (4Alland L. Peseckis S.M. Atherton R.E. Berthiaume L. Resh M.D. J. Biol. Chem. 1994; 269: 16701-16705Abstract Full Text PDF PubMed Google Scholar). COS-1 cells were transfected with FuGene 6 (Roche Molecular Biochemicals) or LipofectAMINE 2000 (Life Technologies, Inc.) according to the manufacturer's instructions. Cells were processed at 48–72 h after transfection. Cells were lysed in RIPA buffer (150 mm NaCl, 1 mm EDTA, 0.1% SDS, 0.5% deoxycholate, 1% Triton X-100, 10 mm Tris, pH 7.4, 1 mm Na3VO4, 10 μg/ml aprotinin, 10 μg/ml leupeptin, 1 mm phenylmethylsulfonyl fluoride). Lysates were clarified at 14,000 rpm in the Eppendorf centrifuge for 15 min at 4 °C. For immunoprecipitation, lysates were incubated with appropriate antibodies and protein A-agarose (Santa Cruz Biotechnology) or formalin-fixed Staphylococcus aureus. Samples were washed three times with RIPA buffer and analyzed by SDS-PAGE. Western blotting was performed using the indicated antibodies. Proteins were detected using horseradish peroxidase-conjugated secondary antibodies and ECL Western blotting detection reagents (Amersham Pharmacia Biotech) following the manufacturer's instructions. For quantitation of Western blots, x-ray films were scanned with an Epson Expression 636 scanner (Epson, Long Beach, CA), and the intensities of the bands were quantified using MacBAS software version 2.0 (Fuji Photo Film Co.; Kohshin Graphic Systems Inc.). Exposure times were adjusted so that the band intensity fell within the linear range of detection. COS-1 cells were incubated in serum-free Dulbecco's modified Eagle's medium for 24 h before the indicated treatments. Cell lysates were prepared as described above. Activation of MAPK was measured by immunoblotting with anti-p-ERK antibody. For cholesterol depletion experiments, cells were incubated with 1% MβCD (dissolved in Dulbecco's modified Eagle's medium) for 1 h at 37 °C before lysis. In other experiments, cells were incubated for 1 h at 37 °C with the indicated inhibitors prior to harvest. Subcellular fractionation was carried out as described previously (4Alland L. Peseckis S.M. Atherton R.E. Berthiaume L. Resh M.D. J. Biol. Chem. 1994; 269: 16701-16705Abstract Full Text PDF PubMed Google Scholar). Briefly, transfected COS-1 cells were lysed by Dounce homogenization in hypotonic buffer. The lysates were subjected to ultracentrifugation at 100,000 × g to generate S100 (soluble) and P100 (membrane) fractions. Each fraction was adjusted to 1× RIPA buffer and immunoprecipitated with anti-Myc antibody followed by Western blotting with anti-Raf antibody. Immunofluorescence staining was carried out as described previously (23Okamura H. Resh M.D. J. Biol. Chem. 1995; 270: 26613-26618Abstract Full Text Full Text PDF PubMed Scopus (102) Google Scholar). COS-1 cells transfected with Raf-1 constructs were seeded onto 25-mm glass coverslips 24 h after transfection. 24 h later, the cells were washed twice with PBS, fixed with 3.7% formalin/PBS for 15 min, and incubated with indicated antibody for 45 min. Cells were washed four times with PBS and incubated with fluorescein isothiocyanate-conjugated secondary antibody for 30 min. Following four 5-min washes, cells were mounted on microscope slides and visualized with a Zeiss Axioplan2 microscope. Membrane raft flotation experiments were performed as described (24Manes S. Mira E. Gomez-Mouton C. Lacalle R.A. Keller P. Labrador J.P. Martinez A.C. EMBO J. 1999; 18: 6211-6220Crossref PubMed Scopus (274) Google Scholar) with minor modifications. Briefly, cells were washed twice with ice-cold 10 mm Tris, pH 7.4, 100 mm NaCl, 1 mm EDTA (STE) and harvested in STE with a rubber policeman. Cell pellets were obtained by centrifugation at 1,000 × g for 5 min at 4 °C. The cell pellet was resuspended in 300 μl of TNET buffer (50 mm Tris-HCl, pH 7.4, 150 mm NaCl, 5 mm EDTA, 0.5% Triton X-100) containing protease inhibitors (1 mmphenylmethylsulfonyl fluoride, 10 μg/ml leupeptin, 10 μg/ml aprotinin), incubated on ice for 20 min, and then adjusted to 35% Optiprep. Cell extracts were overlaid successively with 3.5 ml of 30% Optiprep in TNET and 200 μl of TNET, respectively, in SW55 tubes. Samples were centrifuged for 4 h at 170,000 × gat 4 °C, and five fractions were collected from the top of the gradient. Each fraction was adjusted to 1× RIPA buffer and clarified at 100,000 × g at 4 °C for 15 min. Proteins from each fraction were analyzed by either immunoprecipitation followed by SDS-PAGE or by direct SDS-PAGE and Western blotting. To test whether individual membrane-binding motifs have unique functions in signal transduction, we constructed forms of Raf that were modified by the addition of three different membrane-binding motifs (Fig.1 A). The prenylated tail from K-Ras was appended to the C terminus of Raf, whereas the myristate + basic and myristate + palmitate sequences from Src and Fyn, respectively, were appended to the N terminus of Raf. A Myc epitope tag was added to each construct in order to distinguish the recombinant Raf molecules from endogenous Raf-1. Subcellular fractionation was performed to examine whether Raf constructs tagged with the different membrane-binding motifs were targeted to membranes, by separating transfected COS-1 cells into cytosolic (S100) and membrane (P100) fractions. As depicted in Fig. 1, A and B, Raf molecules containing the various membrane-binding motifs were localized predominantly to the membrane fraction. In contrast, Raf-Myc and Myc-Raf were mainly present in the cytosolic fraction. This demonstrates that the appended membrane binding signals were functional in the context of Raf chimeras. In order to examine whether the Raf constructs tagged with the various membrane-binding motifs can target to the plasma membrane, COS-1 cells transfected with the Raf constructs were subjected to immunofluorescence staining with either anti-Raf or anti-Myc antibodies. The Raf-Myc and Myc-Raf proteins were mainly cytoplasmic, whereas the three Raf constructs tagged with the different membrane-targeting motifs exhibited a typical plasma membrane staining pattern (Fig. 2). Previous studies (21Leevers S.J. Paterson H.F. Marshall C.J. Nature. 1994; 369: 411-414Crossref PubMed Scopus (875) Google Scholar, 22Stokoe D. Macdonald S.G. Cadwallader K. Symons M. Hancock J.F. Science. 1994; 264: 1463-1467Crossref PubMed Scopus (832) Google Scholar) have shown that Raf modified with the K-Ras membrane-binding motif can activate ERK independent of Ras. Here, we compared the biological effects of the three tagged Raf constructs on ERK. Activation of ERK was quantitated by Western blotting with anti-p-ERK antibody (which recognizes only activated ERK) and normalized to the total amount of ERK and Raf construct in each set of transfected cells. The three Raf constructs modified with different membrane-binding domains all caused a more than 10-fold increase in ERK activity, as shown in Fig. 3. Both Myc-Raf and Raf-Myc caused only a slight increase in ERK activity above the background level. These data demonstrate that plasma membrane targeting of Raf is sufficient to activate ERK, consistent with the current model that activation of Raf is due to the recruitment to the plasma membrane by activated Ras (21Leevers S.J. Paterson H.F. Marshall C.J. Nature. 1994; 369: 411-414Crossref PubMed Scopus (875) Google Scholar, 22Stokoe D. Macdonald S.G. Cadwallader K. Symons M. Hancock J.F. Science. 1994; 264: 1463-1467Crossref PubMed Scopus (832) Google Scholar). We next examined the localization of the various modified Raf constructs to rafts. Optiprep density gradient centrifugation was used to separate raft-associated proteins from detergent-sensitive proteins. COS-1 cells transfected with the various Raf constructs were harvested and extracted in ice-cold buffer containing 0.5% Triton X-100. After flotation on Optiprep gradients, five fractions were collected from top to bottom. Rafts are located in the top fraction at the 0/30% interface. The distribution of Raf constructs is shown in Fig.4, A and B. Approximately 60–65% of Fyn-Raf-Myc and Src-Raf-Myc were raft associated. In contrast, only 18% of Myc-Raf-K-Ras partitioned into raft fractions. This pattern is consistent with previous studies indicating that proteins with covalently linked saturated fatty acyl chains are preferentially targeted to rafts, whereas proteins with prenyl groups are excluded (26Melkonian K.A. Ostermeyer A.G. Chen J.Z. Roth M.G. Brown D.A. J. Biol. Chem. 1999; 274: 3910-3917Abstract Full Text Full Text PDF PubMed Scopus (552) Google Scholar). The Raf-Myc and Myc-Raf constructs served as negative controls; these unmodified proteins were found to be excluded completely from rafts. Caveolin-1, an endogenous protein marker for lipid rafts and caveolae, was used as a positive control. As expected, the majority of caveolin-1 was targeted into rafts. The localization of Ha-RasG12V and K-RasG12V, which play an important role in Ras-Raf-MAPK signal transduction pathway, was also analyzed. As depicted in Fig. 4 C, both Ras proteins were almost completely excluded from rafts, consistent with previous studies (26Melkonian K.A. Ostermeyer A.G. Chen J.Z. Roth M.G. Brown D.A. J. Biol. Chem. 1999; 274: 3910-3917Abstract Full Text Full Text PDF PubMed Scopus (552) Google Scholar). Moreover, neither ERK nor p-ERK partitioned into raft fractions. Taken together, these results indicate that the extent of raft localization of the Raf constructs did not directly correlate with the level of ERK activation shown in Fig. 3. We next used the raft disrupting drug MβCD to examine further a potential role of rafts in activation of ERK. COS-1 cells transfected with the various Raf constructs were treated with MβCD for 1 h at 37 °C. Cell lysates were analyzed for activation of ERK by SDS-PAGE and Western blotting with anti-p-ERK antibody. As depicted in Fig.5 A, MβCD had a striking effect on ERK activity, causing a nearly 10-fold increase in p-ERK in mock-transfected, Myc-Raf and Raf-Myc-transfected cells. A similar result was obtained in NIH 3T3 cells, where addition of MβCD caused a 10-fold activation of ERK (Fig. 5 B). In cells expressing the membrane-targeted Raf proteins, there was little to no additional activation of ERK in the presence of MβCD (TableI). Levels of total ERK were the same in all samples.Table IEffect of MβCD on ERK activity mediated by modified Raf constructsConstructsERK activity−MβCD+MβCDS.D.Myc-Raf-K-Ras11.38±0.10Fyn-Raf-Myc11.22±0.04Src-Raf-Myc11.20±0.25COS-1 cells transfected with the indicated Raf constructs were incubated with or without 1% MβCD for 1 h at 37 °C. Activation of ERK was assayed as described under “Experimental Procedures.” ERK activation for each Raf-1 constructs in the absence of MβCD was normalized to 1. S.D., standard deviation. Open table in a new tab COS-1 cells transfected with the indicated Raf constructs were incubated with or without 1% MβCD for 1 h at 37 °C. Activation of ERK was assayed as described under “Experimental Procedures.” ERK activation for each Raf-1 constructs in the absence of MβCD was normalized to 1. S.D., standard deviation. In order to determine whether the effects of MβCD were related to the ability of this compound to bind cholesterol, COS-1 cells were incubated with MβCD in the presence or absence 40 μg/ml cholesterol. As depicted in Fig. 5 C, cholesterol addition prevented ERK activation by MβCD, with the amount of p-ERK reduced to near basal levels. Moreover, addition of another cholesterol-binding agent, filipin, also caused a 4–5-fold activation of ERK (Fig.5 D). These data imply that cholesterol depletion from cell membrane induces ERK activation. The next set of experiments addressed the mechanism by which ERK is activated by MβCD. A dominant negative Ras (Ha-RasN17) construct was used to examine whether activation of ERK by MβCD is dependent on Ras. As depicted in Fig. 6, Ha-RasN17 had no effect on activation of ERK induced by MβCD. In contrast, Ha-RasN17 almost completely inhibited activation of ERK mediated by constitutively activated Ha-Ras (Ha-RasG12V). These results indicate that MβCD-mediated ERK activation occurs through a Ras-independent pathway. Previous studies have indicated a critical role for PI3K in activation of Ras and ERK by epidermal growth factor (27Wennstrom S. Downward J. Mol. Cell. Biol. 1999; 19: 4279-4288Crossref PubMed Scopus (254) Google Scholar, 28Yart A. Laffargue M. Mayeux P. Chretien S. Peres C. Tonks N. Roche S. Payrastre B. Chap H. Raynal P. J. Biol. Chem. 2001; 276: 8856-8864Abstract Full Text Full Text PDF PubMed Scopus (124) Google Scholar). Here, we investigated whether MβCD-induced activation of ERK requires PI3K activity. COS-1 cells were incubated with or without the PI3K inhibitor wortmannin during MβCD treatment. Phosphorylation of endogenous ERK was visualized using anti-p-ERK immunoblotting. Pretreatment with wortmannin almost completely abolished the activation of ERK induced by MβCD, as shown in Fig.7 A. However, wortmannin had no effect on activation of ERK induced by Myc-Raf-K-Ras. The role of PI3K in MβCD-stimulated ERK activation was further confirmed by utilizing LY294002, another inhibitor of PI3K, which is structurally and mechanistically different from wortmannin. As shown in Fig.7 B, treatment of cells with LY294002 inhibited MβCD-induced activation of ERK in a dose-dependent manner. To confirm the data obtained with pharmacological inhibitors, we examined the ability of Δp85, a dominant negative PI3K mutant lacking the p110-binding site, to inhibit MβCD-mediated ERK activation. The activation of ERK was ∼50% (±10%,n = 4) lower when Δp85 was expressed, compared with mock-transfected cells (Fig. 7 C). This level of inhibition correlates with a transfection efficiency of 43% (±6%,n = 6) obtained in these experiments. Finally, activation of PI3K by MβCD was assessed directly, by quantitating the extent of tyrosine phosphorylation of endogenous p85. Phosphorylation of p85 has been shown to correlate with increased PI3K activity (43Pleiman C.M. Hertz W.M. Cambier J.C. Science. 1994; 263: 1609-1612Crossref PubMed Scopus (393) Google Scholar). As depicted in Fig. 7 D, MβCD caused a 3-fold increase in the amount of tyrosine-phosphorylated p85. Taken together, these results indicate that PI3K is required for activation of ERK mediated by MβCD. We next investigated the role of MEK in MβCD-mediated ERK activation. COS-1 cells were incubated with or without 20 μm PD98059 (a specific MEK inhibitor) for 1 h at 37 °C during MβCD treatment. Subsequently, the cells were lysed, and activation of ERK was analyzed by using anti-p-ERK antibody immunoblotting. As shown in Fig. 8, the activation of ERK by MβCD was inhibited by about 50% in the presence of PD98059. In contrast, activation of ERK by Ha-RasG12V and Myc-Raf-K-Ras was nearly completely inhibited by PD98059. All samples contained approximately equal amounts of total ERK. Thus, although MEK is essential for both Ha-RasG12V- and Myc-Raf-K-Ras-mediated ERK activation, activation of ERK by MβCD is only partially dependent on MEK activity. In this paper, we have examined the role of membrane microdomains in activation of MAPK by plasma membrane-bound Raf. There is growing evidence in the literature to indicate that concentration of signaling proteins in membrane microdomains such as rafts and/or caveolae is essential for efficient signal transduction. For example, localization of Src family kinases and LAT to cholesterol, sphingolipid-enriched rafts is required for activation of downstream signaling via the activated T cell receptor (15van't Hof W. Resh M.D. J. Cell Biol. 1999; 145: 377-389Crossref PubMed Scopus (103) Google Scholar, 16Kabouridis P.S. Magee A.I. Ley S.C. EMBO J. 1997; 16: 4983-4998Crossref PubMed Scopus (315) Google Scholar, 17Zhang W. Trible R.P. Samelson L.E. Immunity. 1998; 9: 239-246Abstract Full Text Full Text PDF PubMed Scopus (747) Google Scholar). Other reports have suggested that localization of receptor tyrosine kinases to caveolae is important for stimulation of the MAPK pathway (18Mineo C. James G.L. Smart E.J. Anderson R.G. J. Biol. Chem. 1996; 271: 11930-11935Abstract Full Text Full Text PDF PubMed Scopus (400) Google Scholar, 19Liu P. Ying Y. Ko Y.G. Anderson R.G. J. Biol. Chem. 1996; 271: 10299-10303Abstract Full Text Full Text PDF PubMed Scopus (336) Google Scholar). c-Raf-1, a key intermediate in this pathway, has been reported to be recruited to caveolae upon activation of the epidermal growth factor receptor or Ha-RasG12V (18Mineo C. James G.L. Smart E.J. Anderson R.G. J. Biol. Chem. 1996; 271: 11930-11935Abstract Full Text Full Text PDF PubMed Scopus (400) Google Scholar). Therefore, we tested the role of membrane microdomains by quantitating the ability of three different membrane-targeted Raf-1 constructs to activate MAPK. The amount of p-ERK was normalized to the total amount of each Raf-1 construct and to the total amount of ERK in each set of transfected cells. We found that the extent of MAPK activation by the Src-, Fyn-, and K-Ras-modified Raf proteins was essentially equivalent. It should be noted that expression of an Ha-Ras-tagged Raf construct resulted in 2–3-fold less p-ERK than the other tagged constructs (data not shown). However, indirect immunofluorescence analysis revealed that, in addition to the plasma membrane, a significant amount of Myc-Raf-Ha-Ras was localized to intracellular perinuclear membranes. Perinuclear staining of full-length Ha-Ras, as well as other Ha-Ras-tagged chimeras, has been observed previously and reflects the trafficking of these proteins through the endoplasmic reticulum and Golgi (25Choy E. Chiu V.K. Silletti J. Feoktistov M. Morimoto T. Michaelson D. Ivanov I.E. Philips M.R. Cell. 1999; 98: 69-80Abstract Full Text Full Text PDF PubMed Scopus (612) Google Scholar). Thus, the lower level of p-ERK in these cells may be due to the fact that there is less Myc-Raf-Ha-Ras at the plasma membrane, compared with the other tagged constructs. The extent of MAPK activation did not correlate with the extent of raft localization of the Raf chimeras. As depicted in Fig. 4, the Src and Fyn motifs efficiently targeted the chimeric Raf proteins to rafts, whereas Raf tagged with the prenylated tail from K-Ras was excluded from rafts. The behavior of the chimeric proteins described here mimics that of the full-length proteins; Src and Fyn are enriched in rafts (14Shenoy-Scaria A.M. Dietzen D.J. Kwong J. Link D.C. Lublin D.M. J. Cell Biol. 1994; 126: 353-363Crossref PubMed Scopus (340) Google Scholar, 19Liu P. Ying Y. Ko Y.G. Anderson R.G. J. Biol. Chem. 1996; 271: 10299-10303Abstract Full Text Full Text PDF PubMed Scopus (336) Google Scholar), whereas Ras proteins are largely excluded from rafts (see below) (26Melkonian K.A. Ostermeyer A.G. Chen J.Z. Roth M.G. Brown D.A. J. Biol. Chem. 1999; 274: 3910-3917Abstract Full Text Full Text PDF PubMed Scopus (552) Google Scholar). The raft fractions that were isolated are heterogeneous and include detergent-resistant membranes as well as caveolae. We conclude that, once Raf is membrane-bound, the activation of ERK is raft-independent. The physiological upstream activator of Raf is Ras, which serves to recruit Raf to the plasma membrane. There is now increasing evidence in the literature to indicate that Ras isoforms differ in their abilities to activate downstream effectors. For example, K-Ras activates Raf-1 to a much greater extent than Ha-Ras, whereas Ha-Ras activation of PI3K is greater than that achieved by K-Ras (29Yan J. Roy S. Apolloni A. Lane A. Hancock J.F. J. Biol. Chem. 1998; 273: 24052-24056Abstract Full Text Full Text PDF PubMed Scopus (380) Google Scholar). Moreover, Ha-Ras and K-Ras are localized to different regions of the plasma membrane. K-Ras is distributed throughout the bulk plasma membrane, and activation of Raf by K-Ras is independent of membrane microdomain structure or function. In contrast, the inactive form of Ha-Ras is localized to rafts. Upon binding of GTP, Ha-Ras is released from rafts, a step that is required for full activation of Raf (30Roy S. Luetterforst R. Harding A. Apolloni A. Etheridge M. Stang E. Rolls B. Hancock J.F. Parton R.G. Nat. Cell Biol. 1999; 1: 98-105Crossref PubMed Scopus (124) Google Scholar, 31Prior I.A. Harding A. Yan J. Sluimer J. Parton R.G. Hancock J.F. Nat. Cell Biol. 2001; 3: 368-375Crossref PubMed Scopus (454) Google Scholar). Taken together, these data indicate that Ras-dependent activation of Raf occurs less efficiently in rafts, but once Raf is constitutively membrane-bound, its activation is raft-independent. MβCD is a water-soluble cyclic oligomer that sequesters cholesterol within its hydrophobic core (32Pitha J. Irie T. Sklar P.B. Nye J.S. Life Sci. 1988; 43: 493-502Crossref PubMed Scopus (223) Google Scholar). It has been extensively used as an agent to deplete cholesterol from the plasma membrane of cultured cells (33Neufeld E.B. Cooney A.M. Pitha J. Dawidowicz E.A. Dwyer N.K. Pentchev P.G. Blanchette-Mackie E.J. J. Biol. Chem. 1996; 271: 21604-21613Abstract Full Text Full Text PDF PubMed Scopus (319) Google Scholar). Here we show that MβCD treatment of COS-1 cells results in a dramatic stimulation of MAPK, as reflected in a nearly 10-fold increase in the amount of p-ERK. Stimulation of MAPK by MβCD had been noted previously(20), but the mechanism of activation had not been addressed. In this work, we utilized a combination of pharmacological inhibitors and dominant negative mutants to examine the mechanism of MAPK activation by MβCD. We show that the MβCD effect on MAPK is Ras-independent, as expression of dominant negative Ras had no effect on p-ERK formation in the presence of MβCD (Fig. 6). The MβCD effect occurred downstream or independently from Raf. No additive effect was observed when cells expressing membrane-targeted Raf constructs were treated with MβCD (Table I), and MβCD has no direct effect on Raf activity (30Roy S. Luetterforst R. Harding A. Apolloni A. Etheridge M. Stang E. Rolls B. Hancock J.F. Parton R.G. Nat. Cell Biol. 1999; 1: 98-105Crossref PubMed Scopus (124) Google Scholar). Several lines of evidence support the hypothesis that MβCD activates MAPK via a PI3K-dependent pathway. Treatment of cells with the PI3K inhibitors wortmannin and LY294002 inhibited MβCD-induced activation of MAPK (Fig. 7). Moreover, MAPK activation by MβCD was inhibited when cells were transfected with a dominant negative PI3K mutant, and a 3-fold increase in tyrosine-phosphorylated p85 was observed in MβCD-treated cells. Previous studies have established a close correlation between p85 phosphorylation and PI3K activity (43Pleiman C.M. Hertz W.M. Cambier J.C. Science. 1994; 263: 1609-1612Crossref PubMed Scopus (393) Google Scholar). Activation of PI3K, however, was not sufficient to activate MAPK, as no effect of p110-CAAX, a constitutively activated mutant of PI3K, was observed on p-ERK formation (data not shown). Moreover, MEK was partially required, as concentrations of the MEK-specific inhibitor PD98059 that completely blocked RasG12V activation of MAPK had only partial (50%) inhibitory effects on MβCD activation of MAPK (Fig. 8). At least four mechanisms for activation of MAPK by PI3K-dependent pathways have been reported. Generation of inositol phospholipids by activated PI3K has been shown to stimulate protein kinase C, which then stimulates Raf and activates MAPK (34Le Good J.A. Ziegler W.H. Parekh D.B. Alessi D.R. Cohen P. Parker P.J. Science. 1998; 281: 2042-2045Crossref PubMed Scopus (967) Google Scholar,35Li X. Carter R.H. Eur. J. Immunol. 2000; 30: 1576-1586Crossref PubMed Scopus (19) Google Scholar). PI3K has been shown to activate Rac, which activates MEK via stimulation of p21-activated kinase and Raf (36King A.J. Sun H. Diaz B. Barnard D. Miao W. Bagrodia S. Marshall M.S. Nature. 1998; 396: 180-183Crossref PubMed Scopus (380) Google Scholar). Recently, Yartet al. (28Yart A. Laffargue M. Mayeux P. Chretien S. Peres C. Tonks N. Roche S. Payrastre B. Chap H. Raynal P. J. Biol. Chem. 2001; 276: 8856-8864Abstract Full Text Full Text PDF PubMed Scopus (124) Google Scholar) reported that MAPK activation in epidermal growth factor-stimulated Vero cells was PI3K-dependent; the mechanism involved recruitment of SHP2 and the adaptor protein Gab, upstream of Ras. Finally, G protein-coupled receptors, through Gβγ, have been shown to recruit PI3K to the membrane. This results in activation of Src and the use of an SHC-Grb2-SOS complex to activate Ras and ultimately MAPK (37Gutkind J.S. J. Biol. Chem. 1998; 273: 1839-1842Abstract Full Text Full Text PDF PubMed Scopus (688) Google Scholar, 38Lopez-Ilasaca M. Crespo P. Pellici P.G. Gutkind J.S. Wetzker R. Science. 1997; 275: 394-397Crossref PubMed Scopus (627) Google Scholar). Since MβCD activation of MAPK is independent of Ras (Fig. 6), Raf (30Roy S. Luetterforst R. Harding A. Apolloni A. Etheridge M. Stang E. Rolls B. Hancock J.F. Parton R.G. Nat. Cell Biol. 1999; 1: 98-105Crossref PubMed Scopus (124) Google Scholar), and Src (data not shown), it is unlikely that any of the above pathways are involved in the PI3K-dependent activation of MAPK by MβCD. Rather, we favor the hypothesis that the MβCD activation of MAPK is a consequence of the depletion of plasma membrane cholesterol. The cholesterol-depleting agent filipin also activated MAPK (Fig. 5 D). The MβCD effect on MAPK was blunted by adding back cholesterol (Fig. 5 C), as has also been shown by Furuchi and Anderson (20Furuchi T. Anderson R.G. J. Biol. Chem. 1998; 273: 21099-21104Abstract Full Text Full Text PDF PubMed Scopus (330) Google Scholar). Cholesterol levels in cells are sensed by sterol regulatory element-binding proteins (39Brown M.S. Goldstein J.L. Proc. Natl. Acad. Sci. U. S. A. 1999; 96: 11041-11048Crossref PubMed Scopus (1085) Google Scholar), and it will be interesting to determine whether these endoplasmic reticulum-localized proteins are involved in the MβCD effect on MAPK. Alternatively, PI3K (40Yu W. Cassara J. Weller P.F. Blood. 2000; 95: 1078-1085Crossref PubMed Google Scholar) and MAPK (41Yu W. Bozza P.T. Tzizik D.M. Gray J.P. Cassara J. Dvorak A.M. Weller P.F. Am. J. Pathol. 1998; 152: 759-769PubMed Google Scholar) have been shown to be present on lipid droplets, cytoplasmic storage sites for triacylglycerides, and cholesterol (42van Meer G. J. Cell Biol. 2001; 152: F29-F34Crossref PubMed Scopus (98) Google Scholar). It is tempting to speculate that cholesterol depletion by MβCD might regulate signal transduction by these cytoplasmic organelles. Taken together, the data presented in the current study indicate that depletion of cholesterol from the cell plasma membrane results in a proliferative response and that MβCD has effects on cells beyond disruption of raft and caveolae structure. We thank Xiquan Liang and Wolf Lindwasser for helpful discussions, Raya Louft-Nisenbaum for technical assistance, and Debra Alston for secretarial support." @default.
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