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- W2023293716 abstract "We recently reported the use of covalently attached DNA as a structural constraint for rational control of macromolecular conformation. Reductive amination was employed to attach each strand of the duplex DNA constraint to RNA, utilizing an aldehyde tethered to the 5‘-terminus of the DNA. Here we describe the synthesis of a thymidine phosphoramidite that has the 5‘-tethered aldehyde masked as a 1,2-diol. We also describe optimized reductive amination conditions for linking 5‘-aldehyde-DNA with 2‘-amino-2‘-deoxy-RNA. These procedures should be generally applicable for attaching DNA to biomolecules." @default.
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- W2023293716 date "2006-06-17" @default.
- W2023293716 modified "2023-09-24" @default.
- W2023293716 title "Synthesis and Application of a 5‘-Aldehyde Phosphoramidite for Covalent Attachment of DNA to Biomolecules" @default.
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- W2023293716 doi "https://doi.org/10.1021/jo060723m" @default.
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