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• W2023302073 abstract "Fully localized proton NMR spectra were obtained from the brains of normal anaesthetized rats in vivo using stimulated echo (STEAM) spectroscopy sequences. Investigations were carried out at 2.35 T using a 40 cm bore magnet equipped with an actively shielded gradient system. Localized shimming resulted in water proton linewidths of 6.5-7.8 Hz permitting excellent water suppression. Thus, high-quality proton NMR spectra (TE = 20 ms) were acquired within measuring times of 1.5-6.4 min from 64 to 125 μL volumes-of-interest. The spectra show metabolite resonances due to N aspartate, glutamate, creatine and phosphocreatine, cholines, taurine and inositols. The assignments of strongly spin-coupled resonances were confirmed by comparison with spectra from model solutions obtained under identical experimental conditions to those used in vivo. Tl relaxation times as well as relative metabolite concentrations were evaluated from spectra obtained for repetition times ranging from 900 to 6000 ms. Sequential acquisitions of 1.5 min spectra before, during and after killing the animals exhibited a rapid accumulation of lactate, but did not reveal significant changes in other metabolite levels for several hours post mortem." @default.
• W2023302073 created "2016-06-24" @default.
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• W2023302073 date "1991-06-01" @default.
• W2023302073 modified "2023-10-10" @default.
• W2023302073 title "Non-invasive1H NMR spectroscopy of the rat brainIn Vivo using a short echo time STEAM localization sequence" @default.
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• W2023302073 doi "https://doi.org/10.1002/nbm.1940040307" @default.
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