Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023309543> ?p ?o ?g. }
- W2023309543 endingPage "249" @default.
- W2023309543 startingPage "241" @default.
- W2023309543 abstract "Mammalian cardiomyocytes withdraw from the cell cycle shortly after birth, although it remains unclear how cardiomyocyte cell cycles behave during development. Compared to conventional immunohistochemistry in static observation, time-lapse imaging can reveal comprehensive data in hard-to-understand biological phenomenon. However, there are no reports of an established protocol of successful time-lapse imaging in mammalian heart. Thus, it is valuable to establish a time-lapse imaging system to enable the observation of cell cycle dynamics in living murine cardiomyocytes. This study sought to establish time-lapse imaging of murine heart to study cardiomyocyte cell cycle behavior. The Fucci (fluorescent ubiquitination-based cell cycle indicator) system can effectively label individual G1, S/G2/M, and G1/S-transition phase nuclei red, green and yellow, respectively, in living mammalian cells, and could therefore be useful to visualize the real-time cell cycle transitions in living murine heart. To establish a similar system for time-lapse imaging of murine heart, we first developed an ex vivo culture system, with the culture conditions determined in terms of sample state, serum concentration, and oxygen concentration. The optimal condition (slice culture, oxygen concentration 20%, serum concentration 10%) successfully mimicked physiological cardiomyocyte proliferation in vivo. Time-lapse imaging of cardiac slices from E11.5, E14.5, E18.5, and P1 Fucci-expressing transgenic mice revealed an elongated S/G2/M phase in cardiomyocytes during development. Our time-lapse imaging of murine heart revealed a gradual elongation of the S/G2/M phase during development in living cardiomyocytes." @default.
- W2023309543 created "2016-06-24" @default.
- W2023309543 creator A5003218088 @default.
- W2023309543 creator A5005075596 @default.
- W2023309543 creator A5007788943 @default.
- W2023309543 creator A5020837304 @default.
- W2023309543 creator A5022938409 @default.
- W2023309543 creator A5030430551 @default.
- W2023309543 creator A5031790423 @default.
- W2023309543 creator A5036506634 @default.
- W2023309543 creator A5044204717 @default.
- W2023309543 creator A5051810650 @default.
- W2023309543 creator A5069453818 @default.
- W2023309543 creator A5077065142 @default.
- W2023309543 creator A5077988379 @default.
- W2023309543 creator A5079661753 @default.
- W2023309543 creator A5082913618 @default.
- W2023309543 creator A5091536972 @default.
- W2023309543 date "2014-07-01" @default.
- W2023309543 modified "2023-10-18" @default.
- W2023309543 title "Time-lapse imaging of cell cycle dynamics during development in living cardiomyocyte" @default.
- W2023309543 cites W1547945395 @default.
- W2023309543 cites W1963920189 @default.
- W2023309543 cites W1966817197 @default.
- W2023309543 cites W1967880575 @default.
- W2023309543 cites W1969038150 @default.
- W2023309543 cites W1973706332 @default.
- W2023309543 cites W1973930437 @default.
- W2023309543 cites W1974208182 @default.
- W2023309543 cites W1974433038 @default.
- W2023309543 cites W1979895839 @default.
- W2023309543 cites W1987064856 @default.
- W2023309543 cites W1987343382 @default.
- W2023309543 cites W1993146491 @default.
- W2023309543 cites W1994143216 @default.
- W2023309543 cites W1996435746 @default.
- W2023309543 cites W1998342517 @default.
- W2023309543 cites W2000156766 @default.
- W2023309543 cites W2000157901 @default.
- W2023309543 cites W2005915392 @default.
- W2023309543 cites W2012877726 @default.
- W2023309543 cites W2023453764 @default.
- W2023309543 cites W2031125174 @default.
- W2023309543 cites W2032189169 @default.
- W2023309543 cites W2035033869 @default.
- W2023309543 cites W2036009098 @default.
- W2023309543 cites W2042742511 @default.
- W2023309543 cites W2048616114 @default.
- W2023309543 cites W2056483214 @default.
- W2023309543 cites W2064178384 @default.
- W2023309543 cites W2072673889 @default.
- W2023309543 cites W2085103039 @default.
- W2023309543 cites W2085812650 @default.
- W2023309543 cites W2089247555 @default.
- W2023309543 cites W2098030130 @default.
- W2023309543 cites W2100164332 @default.
- W2023309543 cites W2102525690 @default.
- W2023309543 cites W2103272211 @default.
- W2023309543 cites W2108408221 @default.
- W2023309543 cites W2112055300 @default.
- W2023309543 cites W2117767933 @default.
- W2023309543 cites W2121524151 @default.
- W2023309543 cites W2124955671 @default.
- W2023309543 cites W2128429462 @default.
- W2023309543 cites W2140619873 @default.
- W2023309543 cites W2150501736 @default.
- W2023309543 cites W2160597085 @default.
- W2023309543 cites W2167179112 @default.
- W2023309543 cites W2167993476 @default.
- W2023309543 cites W4211016865 @default.
- W2023309543 doi "https://doi.org/10.1016/j.yjmcc.2014.03.020" @default.
- W2023309543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24704900" @default.
- W2023309543 hasPublicationYear "2014" @default.
- W2023309543 type Work @default.
- W2023309543 sameAs 2023309543 @default.
- W2023309543 citedByCount "27" @default.
- W2023309543 countsByYear W20233095432014 @default.
- W2023309543 countsByYear W20233095432015 @default.
- W2023309543 countsByYear W20233095432016 @default.
- W2023309543 countsByYear W20233095432017 @default.
- W2023309543 countsByYear W20233095432018 @default.
- W2023309543 countsByYear W20233095432019 @default.
- W2023309543 countsByYear W20233095432020 @default.
- W2023309543 countsByYear W20233095432021 @default.
- W2023309543 countsByYear W20233095432022 @default.
- W2023309543 countsByYear W20233095432023 @default.
- W2023309543 crossrefType "journal-article" @default.
- W2023309543 hasAuthorship W2023309543A5003218088 @default.
- W2023309543 hasAuthorship W2023309543A5005075596 @default.
- W2023309543 hasAuthorship W2023309543A5007788943 @default.
- W2023309543 hasAuthorship W2023309543A5020837304 @default.
- W2023309543 hasAuthorship W2023309543A5022938409 @default.
- W2023309543 hasAuthorship W2023309543A5030430551 @default.
- W2023309543 hasAuthorship W2023309543A5031790423 @default.
- W2023309543 hasAuthorship W2023309543A5036506634 @default.
- W2023309543 hasAuthorship W2023309543A5044204717 @default.
- W2023309543 hasAuthorship W2023309543A5051810650 @default.
- W2023309543 hasAuthorship W2023309543A5069453818 @default.