FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023312144> ?p ?o ?g. }

• W2023312144 endingPage "1828" @default.
• W2023312144 startingPage "1818" @default.
• W2023312144 abstract "The labeling of products containing genetically modified organisms (GMO) is linked to their quantification since a threshold for the presence of fortuitous GMOs in food has been established. This threshold is calculated from a combination of two absolute quantification values: one for the specific GMO target and the second for an endogenous reference gene specific to the taxon. Thus, the development of reliable methods to quantify GMOs using endogenous reference genes in complex matrixes such as food and feed is needed. Plant identification can be difficult in the case of closely related taxa, which moreover are subject to introgression events. Based on the homology of β-fructosidase sequences obtained from public databases, two couples of consensus primers were designed for the detection, quantification, and differentiation of four Solanaceae: potato (Solanum tuberosum), tomato (Solanum lycopersicum), pepper (Capsicum annuum), and eggplant (Solanum melongena). Sequence variability was studied first using lines and cultivars (intraspecies sequence variability), then using taxa involved in gene introgressions, and finally, using taxonomically close taxa (interspecies sequence variability). This study allowed us to design four highly specific TaqMan-MGB probes. A duplex real time PCR assay was developed for simultaneous quantification of tomato and potato. For eggplant and pepper, only simplex real time PCR tests were developed. The results demonstrated the high specificity and sensitivity of the assays. We therefore conclude that β-fructosidase can be used as an endogenous reference gene for GMO analysis." @default.
• W2023312144 created "2016-06-24" @default.
• W2023312144 creator A5023829513 @default.
• W2023312144 creator A5033612867 @default.
• W2023312144 creator A5055073960 @default.
• W2023312144 creator A5074573334 @default.
• W2023312144 creator A5078353536 @default.
• W2023312144 creator A5088390625 @default.
• W2023312144 creator A5091412703 @default.
• W2023312144 date "2008-02-28" @default.
• W2023312144 modified "2023-10-18" @default.
• W2023312144 title "Development of a Real-Time PCR Method for the Differential Detection and Quantification of Four Solanaceae in GMO Analysis: Potato (Solanum Tuberosum), Tomato (Solanum Lycopersicum), Eggplant (Solanum Melongena), and Pepper (Capsicum Annuum)" @default.
• W2023312144 cites W1965017751 @default.
• W2023312144 cites W1967215284 @default.
• W2023312144 cites W1976804125 @default.
• W2023312144 cites W1983339768 @default.
• W2023312144 cites W1999065765 @default.
• W2023312144 cites W1999484663 @default.
• W2023312144 cites W2016138904 @default.
• W2023312144 cites W2017829077 @default.
• W2023312144 cites W2022619272 @default.
• W2023312144 cites W2025752088 @default.
• W2023312144 cites W2026496972 @default.
• W2023312144 cites W2029417763 @default.
• W2023312144 cites W2034767282 @default.
• W2023312144 cites W2035443736 @default.
• W2023312144 cites W2036791689 @default.
• W2023312144 cites W2051366482 @default.
• W2023312144 cites W2051972372 @default.
• W2023312144 cites W2063448449 @default.
• W2023312144 cites W2065589216 @default.
• W2023312144 cites W2067720953 @default.
• W2023312144 cites W2081416343 @default.
• W2023312144 cites W2086767221 @default.
• W2023312144 cites W2088333208 @default.
• W2023312144 cites W2100204796 @default.
• W2023312144 cites W2101505606 @default.
• W2023312144 cites W2102222986 @default.
• W2023312144 cites W2122766746 @default.
• W2023312144 cites W2126032575 @default.
• W2023312144 cites W2136952339 @default.
• W2023312144 cites W2139658335 @default.
• W2023312144 cites W2149775275 @default.
• W2023312144 cites W2153492710 @default.
• W2023312144 cites W2164095252 @default.
• W2023312144 cites W2167219490 @default.
• W2023312144 cites W2249548624 @default.
• W2023312144 cites W2259640109 @default.
• W2023312144 cites W2319915860 @default.
• W2023312144 cites W2322387218 @default.
• W2023312144 cites W2322974539 @default.
• W2023312144 cites W3023814608 @default.
• W2023312144 doi "https://doi.org/10.1021/jf073313n" @default.
• W2023312144 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18303841" @default.
• W2023312144 hasPublicationYear "2008" @default.
• W2023312144 type Work @default.
• W2023312144 sameAs 2023312144 @default.
• W2023312144 citedByCount "41" @default.
• W2023312144 countsByYear W20233121442012 @default.
• W2023312144 countsByYear W20233121442013 @default.
• W2023312144 countsByYear W20233121442014 @default.
• W2023312144 countsByYear W20233121442016 @default.
• W2023312144 countsByYear W20233121442017 @default.
• W2023312144 countsByYear W20233121442018 @default.
• W2023312144 countsByYear W20233121442020 @default.
• W2023312144 countsByYear W20233121442021 @default.
• W2023312144 crossrefType "journal-article" @default.
• W2023312144 hasAuthorship W2023312144A5023829513 @default.
• W2023312144 hasAuthorship W2023312144A5033612867 @default.
• W2023312144 hasAuthorship W2023312144A5055073960 @default.
• W2023312144 hasAuthorship W2023312144A5074573334 @default.
• W2023312144 hasAuthorship W2023312144A5078353536 @default.
• W2023312144 hasAuthorship W2023312144A5088390625 @default.
• W2023312144 hasAuthorship W2023312144A5091412703 @default.
• W2023312144 hasConcept C104317684 @default.
• W2023312144 hasConcept C144027150 @default.
• W2023312144 hasConcept C2776242653 @default.
• W2023312144 hasConcept C2776537196 @default.
• W2023312144 hasConcept C2778761015 @default.
• W2023312144 hasConcept C2781083041 @default.
• W2023312144 hasConcept C2993140171 @default.
• W2023312144 hasConcept C54355233 @default.
• W2023312144 hasConcept C59822182 @default.
• W2023312144 hasConcept C8182607 @default.
• W2023312144 hasConcept C86803240 @default.
• W2023312144 hasConceptScore W2023312144C104317684 @default.
• W2023312144 hasConceptScore W2023312144C144027150 @default.
• W2023312144 hasConceptScore W2023312144C2776242653 @default.
• W2023312144 hasConceptScore W2023312144C2776537196 @default.
• W2023312144 hasConceptScore W2023312144C2778761015 @default.
• W2023312144 hasConceptScore W2023312144C2781083041 @default.
• W2023312144 hasConceptScore W2023312144C2993140171 @default.
• W2023312144 hasConceptScore W2023312144C54355233 @default.
• W2023312144 hasConceptScore W2023312144C59822182 @default.
• W2023312144 hasConceptScore W2023312144C8182607 @default.
• W2023312144 hasConceptScore W2023312144C86803240 @default.
• W2023312144 hasIssue "6" @default.
• W2023312144 hasLocation W20233121441 @default.

• next