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- W2023322095 abstract "DNA fingerprinting is a molecular biological technique that is widely used for identifying parentage and relatedness in plants and animals. To identify new DNA fingerprinting probes for use with salmonids, Southern blots of genomic DNA from chinook salmon (Oncorhynchus tshawytscha) were hybridized at low stringencies with 12 different oligonucleotides designed from published core sequences of variable number of tendem repeats. Seven of the 12 oligonucleotides produced highly variable fingerprint-like patterns; however, only 3 of these had clear, distinct bands. The estimated heterozygosity for one population of chinook salmon using the three oligonucleotides as probes ranged from 0.64 to 0.77. Those three oligonucleotides were further hybridized with DNA from two unrelated individuals from six other species of salmonids. A single-locus DNA fingerprint probe originally developed for chinook salmon was also hybridized with DNA from the other six species at moderate stringency. There were differences in the complexity and signal strength of the resulting banding pattern between species for a given probe. Estimates of variability (heterozygosity and band sharing) for the three oligonucleotide probes and OtSL1 were high, indicating that the probes were potentially useful genetic markers. The availability of these additional DNA fingerprint probes should assist in ecological and evolutionary studies in salmonids, as well as in efforts to estimate genetic diversity of populations." @default.
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- W2023322095 date "1995-03-01" @default.
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- W2023322095 title "Multilocus DNA fingerprints in seven species of salmonids" @default.
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