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• W2023341510 abstract "Stem cell therapy holds great promise for the treatment of injured myocardium, but is challenged by a limited supply of appropriate cells. Three different isoforms of transforming growth factor-β (TGF-β) -β1, -β2, and -β3 exhibit distinct regulatory effects on cell growth, differentiation, and migration during embryonic development. We compared the effects of these three different isoforms on cardiomyocyte differentiation from embryonic stem (ES) cells. In contrast to TGF-β1, or -β3, treatment of mouse ES cells with TGF-β2 isoform significantly increased embryoid body (EB) proliferation as well as the extent of the EB outgrowth that beat rhythmically. At 17 days, 49% of the EBs treated with TGF-β2 exhibited spontaneous beating compared with 15% in controls. Cardiac myocyte specific protein markers sarcomeric myosin and α-actin were demonstrated in beating EBs and cells isolated from EBs. In conclusion, TGF-β2 but not TGF-β1, or -β3 promotes cardiac myocyte differentiation from ES cells." @default.
• W2023341510 created "2016-06-24" @default.
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• W2023341510 creator A5036977291 @default.
• W2023341510 date "2005-06-01" @default.
• W2023341510 modified "2023-10-18" @default.
• W2023341510 title "Transforming growth factor-β2 enhances differentiation of cardiac myocytes from embryonic stem cells" @default.
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• W2023341510 doi "https://doi.org/10.1016/j.bbrc.2005.04.098" @default.
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