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• W2023343628 startingPage "1112" @default.
• W2023343628 abstract "We have developed an in vitro transcription system that uses nuclei isolated from Lemna gibba G-3. The in vitro transcripts include sequences homologous to hybridization probes for the small subunit of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39], the light-harvesting chlorophyll a / b -protein, and rRNA. Light-harvesting chlorophyll a / b -protein sequences are transcribed to a greater extent in nuclei isolated from plants grown in darkness with 2 min of red light every 8 hr than in nuclei isolated from dark-treated plants. Furthermore, the amount of these transcripts measured in plants given a single minute of red light after dark treatment is increased over the amount measured in dark-treated plants. The effect of red light is at least partially reversible by 10 min of far-red light given immediately after the red light pulse. Transcription of both rRNA and small subunit sequences is also stimulated by a single minute of red light as compared to dark-treated tissue. However, the relative magnitudes of the increases compared to the dark levels are smaller than the increase seen for the chlorophyll a / b -protein, possibly because of the higher level of transcription of these sequences in the dark. The effect of red light on the transcription of small subunit and rRNA sequences is also reversible by immediate treatment with 10 min of far-red light. Pulse chase studies of dark-treated nuclei for up to 110 min do not show substantial turnover of in vitro labeled small subunit and chlorophyll a / b -protein transcripts. We therefore conclude that phytochrome action has induced specific changes in transcription of these genes." @default.
• W2023343628 created "2016-06-24" @default.
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• W2023343628 date "1984-02-01" @default.
• W2023343628 modified "2023-10-18" @default.
• W2023343628 title "Demonstration of transcriptional regulation of specific genes by phytochrome action" @default.
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• W2023343628 doi "https://doi.org/10.1073/pnas.81.4.1112" @default.
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