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- W2023349507 abstract "Two herbicide-resistant mutants of the unicellular cyanobacterium, Anacystis nidulans R2, were obtained by mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine. These mutants, A. nidulans R2D1 and R2D2, were selected by growth of mutagenized cells in the presence of 10−6 M and 10−5 M 3-(3,4-dichlorophenyl)-1,1-dimethyl urea (DCMU), respectively. Both were found to be cross-resistant to 2-chloro-4-ethylamino-6-isopropylamino-s-triazine (atrazine) and 2-n-heptyl-4-hydroxyquinoline-n-oxide (HQNO) by measurement of Photosystem II activity in the presence of the inhibitors. The DCMU-resistance trait from each mutant was transferred to a wild-type genetic background by DNA-mediated transformation of A. nidulans cells. The two resulting transformants, A. nidulans R2D1-X1 and R2D2-X1, were similar to the original mutants with respect to DCMU- and HQNO-resistance. However, both exhibited increased sensitivity to atrazine relative to the mutants from which they were derived. Polyacrylamide gel electrophoretic analysis revealed that the mutants and transformants were deficient in a 34 kDa, surface-exposed polypeptide which was present in the wild-type strain; the transformants exhibited a new polypeptide of 35.5 kDa which was also highly surface-exposed." @default.
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- W2023349507 date "1984-02-01" @default.
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- W2023349507 title "Biochemical and biophysical characterization of herbicide-resistant mutants of the unicellular cyanobacterium, Anacystis nidulans R2" @default.
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- W2023349507 doi "https://doi.org/10.1016/0005-2728(84)90033-1" @default.
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