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- W2023351889 endingPage "28" @default.
- W2023351889 startingPage "15" @default.
- W2023351889 abstract "The study of genetics, genes, and chromosomal inheritance was initiated by Thomas Morgan in 1910, when the first visible mutations were identified in fruit flies. The field expanded upon the work initiated by Herman Muller in 1926 when he used X-rays to develop the first balancer chromosomes. Today, balancers are still invaluable to maintain mutations and transgenes but the arsenal of tools has expanded vastly and numerous new methods have been developed, many relying on the availability of the genome sequence and transposable elements. Forward genetic screens based on chemical mutagenesis or transposable elements have resulted in the unbiased identification of many novel players involved in processes probed by specific phenotypic assays. Reverse genetic approaches have relied on the availability of a carefully selected set of transposon insertions spread throughout the genome to allow the manipulation of the region in the vicinity of each insertion. Lastly, the ability to transform Drosophila with single copy transgenes using transposons or site-specific integration using the ΦC31 integrase has allowed numerous manipulations, including the ability to create and integrate genomic rescue constructs, generate duplications, RNAi knock-out technology, binary expression systems like the GAL4/UAS system as well as other methods. Here, we will discuss the most useful methodologies to interrogate the fruit fly genome in vivo focusing on chemical mutagenesis, transposons and transgenes. Genome engineering approaches based on nucleases and RNAi technology are discussed in following chapters." @default.
- W2023351889 created "2016-06-24" @default.
- W2023351889 creator A5026995692 @default.
- W2023351889 creator A5070967674 @default.
- W2023351889 date "2014-06-01" @default.
- W2023351889 modified "2023-09-28" @default.
- W2023351889 title "Chemical mutagens, transposons, and transgenes to interrogate gene function in Drosophila melanogaster" @default.
- W2023351889 cites W1528687531 @default.
- W2023351889 cites W1867326234 @default.
- W2023351889 cites W1906155566 @default.
- W2023351889 cites W1921323345 @default.
- W2023351889 cites W1923253031 @default.
- W2023351889 cites W1938103828 @default.
- W2023351889 cites W1939841200 @default.
- W2023351889 cites W1965769301 @default.
- W2023351889 cites W1966085961 @default.
- W2023351889 cites W1966309208 @default.
- W2023351889 cites W1967415231 @default.
- W2023351889 cites W1969436175 @default.
- W2023351889 cites W1974750801 @default.
- W2023351889 cites W1976557090 @default.
- W2023351889 cites W1978157456 @default.
- W2023351889 cites W1978245763 @default.
- W2023351889 cites W1979400866 @default.
- W2023351889 cites W1980333298 @default.
- W2023351889 cites W1987291027 @default.
- W2023351889 cites W1989141400 @default.
- W2023351889 cites W1989322815 @default.
- W2023351889 cites W1993445706 @default.
- W2023351889 cites W1994251246 @default.
- W2023351889 cites W1994337546 @default.
- W2023351889 cites W1994528729 @default.
- W2023351889 cites W2000572318 @default.
- W2023351889 cites W2001488804 @default.
- W2023351889 cites W2003785972 @default.
- W2023351889 cites W2004666307 @default.
- W2023351889 cites W2006602385 @default.
- W2023351889 cites W2008276103 @default.
- W2023351889 cites W2008555242 @default.
- W2023351889 cites W2010747656 @default.
- W2023351889 cites W2012674191 @default.
- W2023351889 cites W2013257515 @default.
- W2023351889 cites W2013829705 @default.
- W2023351889 cites W2018438969 @default.
- W2023351889 cites W2019205338 @default.
- W2023351889 cites W2020808982 @default.
- W2023351889 cites W2021373833 @default.
- W2023351889 cites W2021892293 @default.
- W2023351889 cites W2026386725 @default.
- W2023351889 cites W2027125425 @default.
- W2023351889 cites W2028464990 @default.
- W2023351889 cites W2029880048 @default.
- W2023351889 cites W2030522563 @default.
- W2023351889 cites W2032118018 @default.
- W2023351889 cites W2032972160 @default.
- W2023351889 cites W2034108485 @default.
- W2023351889 cites W2036338354 @default.
- W2023351889 cites W2036407495 @default.
- W2023351889 cites W2037366394 @default.
- W2023351889 cites W2037611829 @default.
- W2023351889 cites W2038330247 @default.
- W2023351889 cites W2041330970 @default.
- W2023351889 cites W2042358820 @default.
- W2023351889 cites W2043350391 @default.
- W2023351889 cites W2044594834 @default.
- W2023351889 cites W2044922004 @default.
- W2023351889 cites W2046661558 @default.
- W2023351889 cites W2052024371 @default.
- W2023351889 cites W2052112605 @default.
- W2023351889 cites W2052646018 @default.
- W2023351889 cites W2056900382 @default.
- W2023351889 cites W2060089435 @default.
- W2023351889 cites W2063834599 @default.
- W2023351889 cites W2068707689 @default.
- W2023351889 cites W2071344712 @default.
- W2023351889 cites W2072354358 @default.
- W2023351889 cites W2073036589 @default.
- W2023351889 cites W2074430735 @default.
- W2023351889 cites W2080685236 @default.
- W2023351889 cites W2083168105 @default.
- W2023351889 cites W2085107624 @default.
- W2023351889 cites W2086689509 @default.
- W2023351889 cites W2088960975 @default.
- W2023351889 cites W2090976240 @default.
- W2023351889 cites W2091869268 @default.
- W2023351889 cites W2092098765 @default.
- W2023351889 cites W2096454034 @default.
- W2023351889 cites W2100270425 @default.
- W2023351889 cites W2102847484 @default.
- W2023351889 cites W2103046819 @default.
- W2023351889 cites W2103080800 @default.
- W2023351889 cites W2103100126 @default.
- W2023351889 cites W2103587834 @default.
- W2023351889 cites W2104582907 @default.
- W2023351889 cites W2105174770 @default.
- W2023351889 cites W2106011172 @default.
- W2023351889 cites W2106029142 @default.
- W2023351889 cites W2107396888 @default.