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- W2023352149 abstract "Interserotypic recombinants are formed in crosses between temperature-sensitive (ts) mutants of adenovirus type 5 (Ad5) and the nondefective adenovirus 2-SV40 hybrid virus Ad2+ND1. The positions of Ad2+ND1 and Ad5 DNA sequences in recombinant genomes can be determined by analysis of their DNA with restriction endonucleases and the sites of the mutation in many ts mutants have been mapped [Grodzicker, T., Williams, J. F., Sharp, P. A., and Sambrook, J. (1974), Cold Spring Harbor Zymp. Quant. Biol. 39, 439–446; Sambrook, J., Williams, J., Sharp, P. A., and Grodzicker, T. (1975), J. Mol. Biol. 97, 369–390]. We show here that several Ad5 polypeptides can be distinguished from their Ad2+ND1 counterparts by SDS-polyacrylamide gel electrophoresis. Analysis of the polypeptides encoded by recombinants of known genetic constitution has allowed us to locate several structural genes on the adenovirus genetic and physical maps and to assign ts mutants to specific genes. Furthermore, we have physically mapped the early Ad5 mutant ts 125 which has a defect in the gene for the adenovirus-specific single-stranded binding protein. In addition, we show that the virus-associated RNAs of Ad2 and Ad5 can be separated electrophoretically, permitting rapid and simple determination of the origin of the DNA surrounding position 0.30 in interserotypic recombinants." @default.
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- W2023352149 date "1977-07-01" @default.
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- W2023352149 title "The physical locations of structural genes in adenovirus DNA" @default.
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- W2023352149 doi "https://doi.org/10.1016/0042-6822(77)90384-1" @default.
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