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- W2023353082 abstract "The applicability of capillary zone electrophoresis for the simultaneous determination of cefamandole and probenecid in body fluids has been studied using salicylic acid as an internal standard. A 10 mM disodium tetraborate solution (pH 8.0) was used as a running buffer and the UV wavelength was set at 230 nm for monitoring the analytes at a separation voltage of 18 kV. Under the optimized conditions, the analytes can be effectively separated in 6 min. The standard curves of cefamandole and probenecid showed good linearity in the range of 10–200 and 5–110 μg mL−1, respectively, with correlation coefficients r > 0.999. The detection limits (S/N = 3) of cefamandole and probenecid were 2.7 and 1.1 μg mL−1, respectively. Intra- and inter-day analytical precisions (relative standard deviation, RSD) of peak area and migration time for the two medicines were both less than 2%. The recoveries at three spiked levels of cefamandole and probenecid from urine and serum samples were in the range of 96.23–105.1% with RSD values of 0.55–2.36%. The proposed method was tested for clinical application by the simultaneous determination of cefamandole and probenecid in human urine and serum with satisfactory results." @default.
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- W2023353082 date "2013-01-01" @default.
- W2023353082 modified "2023-09-25" @default.
- W2023353082 title "Effective separation and simultaneous determination of cefamandole and probenecid in body fluids by capillary zone electrophoresis with salicylic acid as an internal standard" @default.
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- W2023353082 doi "https://doi.org/10.1039/c3ay40956b" @default.
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