FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023353089> ?p ?o ?g. }

• W2023353089 endingPage "696" @default.
• W2023353089 startingPage "690" @default.
• W2023353089 abstract "Identification and selective labeling of the melibiose permease and alpha-galactosidase in Escherichia coli, which are encoded by the melB and melA genes, respectively, have been accomplished by selectively labeling the two gene products with a T7 RNA polymerase expression system [Tabor, S., & Richardson, C. C. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 1074]. Following generation of a novel EcoRI restriction site in the intergenic sequence between the two genes of the mel operon by oligonucleotide-directed, site-specific mutagenesis, melA and melB were separately inserted into plasmid pT7-6 of the T7 expression system. Expression of melB was markedly enhanced by placing a strong, synthetic ribosome binding site at an optimal distance upstream from the initiation codon of melB. Expression of cloned gene products was characterized functionally and by performing autoradiographic analysis on total cell, inner membrane, and cytoplasmic proteins from cells pulse labeled with (35S)methionine in the presence of rifampicin and resolved by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The results first confirm that alpha-galactosidase is a cytoplasmic protein with an Mr of 50K; in contrast, the membrane-bound melibiose permease is identified as a protein with an apparent Mr of 39K, a value significantly higher than that of 30K previously suggested [Hanatani et al. (1984) J. Biol. Chem. 259, 1807]." @default.
• W2023353089 created "2016-06-24" @default.
• W2023353089 creator A5013090368 @default.
• W2023353089 creator A5025160383 @default.
• W2023353089 creator A5026835355 @default.
• W2023353089 creator A5050461931 @default.
• W2023353089 creator A5083844882 @default.
• W2023353089 date "1990-01-23" @default.
• W2023353089 modified "2023-10-16" @default.
• W2023353089 title "Melibiose permease and .alpha.-galactosidase of Escherichia coli: identification by selective labeling using a T7 RNA polymerase/promoter expression system" @default.
• W2023353089 cites W1409116902 @default.
• W2023353089 cites W1481718128 @default.
• W2023353089 cites W1488453787 @default.
• W2023353089 cites W1551407530 @default.
• W2023353089 cites W1567963927 @default.
• W2023353089 cites W1588946648 @default.
• W2023353089 cites W1589773429 @default.
• W2023353089 cites W1597225811 @default.
• W2023353089 cites W1604881631 @default.
• W2023353089 cites W1606707893 @default.
• W2023353089 cites W1607829040 @default.
• W2023353089 cites W1789416382 @default.
• W2023353089 cites W193030898 @default.
• W2023353089 cites W1968130621 @default.
• W2023353089 cites W1974988689 @default.
• W2023353089 cites W2008090841 @default.
• W2023353089 cites W2019352025 @default.
• W2023353089 cites W2020755998 @default.
• W2023353089 cites W2045676450 @default.
• W2023353089 cites W2048468042 @default.
• W2023353089 cites W2052171339 @default.
• W2023353089 cites W2066739041 @default.
• W2023353089 cites W2068518785 @default.
• W2023353089 cites W2069573006 @default.
• W2023353089 cites W2069637664 @default.
• W2023353089 cites W2086733743 @default.
• W2023353089 cites W2090737357 @default.
• W2023353089 cites W2100837269 @default.
• W2023353089 cites W2125796209 @default.
• W2023353089 cites W2136252882 @default.
• W2023353089 cites W2138270253 @default.
• W2023353089 cites W2138840910 @default.
• W2023353089 cites W2156930003 @default.
• W2023353089 cites W2157690667 @default.
• W2023353089 cites W2166064969 @default.
• W2023353089 cites W2202172284 @default.
• W2023353089 cites W2219208845 @default.
• W2023353089 cites W64631016 @default.
• W2023353089 cites W98755409 @default.
• W2023353089 cites W2090920017 @default.
• W2023353089 doi "https://doi.org/10.1021/bi00455a014" @default.
• W2023353089 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2159786" @default.
• W2023353089 hasPublicationYear "1990" @default.
• W2023353089 type Work @default.
• W2023353089 sameAs 2023353089 @default.
• W2023353089 citedByCount "16" @default.
• W2023353089 countsByYear W20233530892017 @default.
• W2023353089 crossrefType "journal-article" @default.
• W2023353089 hasAuthorship W2023353089A5013090368 @default.
• W2023353089 hasAuthorship W2023353089A5025160383 @default.
• W2023353089 hasAuthorship W2023353089A5026835355 @default.
• W2023353089 hasAuthorship W2023353089A5050461931 @default.
• W2023353089 hasAuthorship W2023353089A5083844882 @default.
• W2023353089 hasConcept C104317684 @default.
• W2023353089 hasConcept C113629113 @default.
• W2023353089 hasConcept C114847915 @default.
• W2023353089 hasConcept C153911025 @default.
• W2023353089 hasConcept C170344550 @default.
• W2023353089 hasConcept C185592680 @default.
• W2023353089 hasConcept C203075996 @default.
• W2023353089 hasConcept C2776441376 @default.
• W2023353089 hasConcept C2776449523 @default.
• W2023353089 hasConcept C2780048736 @default.
• W2023353089 hasConcept C547475151 @default.
• W2023353089 hasConcept C55493867 @default.
• W2023353089 hasConcept C67705224 @default.
• W2023353089 hasConcept C86803240 @default.
• W2023353089 hasConcept C88478588 @default.
• W2023353089 hasConceptScore W2023353089C104317684 @default.
• W2023353089 hasConceptScore W2023353089C113629113 @default.
• W2023353089 hasConceptScore W2023353089C114847915 @default.
• W2023353089 hasConceptScore W2023353089C153911025 @default.
• W2023353089 hasConceptScore W2023353089C170344550 @default.
• W2023353089 hasConceptScore W2023353089C185592680 @default.
• W2023353089 hasConceptScore W2023353089C203075996 @default.
• W2023353089 hasConceptScore W2023353089C2776441376 @default.
• W2023353089 hasConceptScore W2023353089C2776449523 @default.
• W2023353089 hasConceptScore W2023353089C2780048736 @default.
• W2023353089 hasConceptScore W2023353089C547475151 @default.
• W2023353089 hasConceptScore W2023353089C55493867 @default.
• W2023353089 hasConceptScore W2023353089C67705224 @default.
• W2023353089 hasConceptScore W2023353089C86803240 @default.
• W2023353089 hasConceptScore W2023353089C88478588 @default.
• W2023353089 hasIssue "3" @default.
• W2023353089 hasLocation W20233530891 @default.
• W2023353089 hasLocation W20233530892 @default.
• W2023353089 hasOpenAccess W2023353089 @default.
• W2023353089 hasPrimaryLocation W20233530891 @default.

• next