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- W2023353531 abstract "Alginate-coated shoot tips from in vitro-grown apple (Malus domestica Borkh cv. Fuji) were successfully cryopreserved following dehydration. Shoot tips cold-hardened at 5°C for 3 weeks, were progressively precultured on MS agar media with increasing sucrose (0.1,0.4 and 0.7M) daily at 5°C. The precultured shoot tips trapped into alginate-coated beads containing 0.5 M sucrose were treated in a medium supplemented with 1.0 M sucrose for 16 h at 5°C. Beads containing 1 shoot tip were then dehydrated up to about 33% water content (fresh weight basis) on sterile dry silica gel at 25°C before being immersed in liquid nitrogen (LN). The average rate of shoot formation after warming was about 80%. This method was successfully applied to three apple, one mulberry and three pear species or cultivars. This encapsulation-dehydration method also permitted the shoot tips to be stored at -135°C for 5 months with little or no decrease in the rate of shoot formation. This modified method appears to be a promising technique for cryopreserving shoot tips from in vitro-grown plantlets of deciduous trees." @default.
- W2023353531 created "2016-06-24" @default.
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- W2023353531 date "1992-01-01" @default.
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- W2023353531 title "Cryopreservation of alginate-coated in vitro-grown shoot tips of apple, pear and mulberry" @default.
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- W2023353531 doi "https://doi.org/10.1016/0168-9452(92)90151-b" @default.
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