Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023354858> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2023354858 endingPage "631" @default.
- W2023354858 startingPage "623" @default.
- W2023354858 abstract "A filter paper assay was developed for measuring the activity of transamidating enzymes by the incorporation of radioactive amine substrates into proteins. The method is analogous to those generally employed for monitoring the enzymic coupling of isotopic groups to proteins, such as in the ATP-dependent phosphorylation of casein or histone. Use of minimal quantities of reagents, as well as simplicity of handling a large number of test samples, are distinct advantages of this technique. The guinea pig liver transglutaminase-catalyzed incorporation of C14-histamine and C14-putrescine into β-lactoglobulin and casein were studied. The apparent Michaelis kinetics of the reactions permitted evaluation of Km,app's for both isotopic substrates and competitive inhibition constants for nonradioactive amines were also obtained. In contrast to transglutaminase, the fibrinoligase—generated in human plasma from Factor XIII by the addition of thrombin—could not utilize β-lactoglobulin to an appreciable extent. Hence experiments had to be restricted to casein as acceptor protein. Apparent Michaelis constants for the isotopic substrates and inhibition constants for nonradioactive amines could be readily measured also in the complex plasma system. Finally, details of a rapid filter paper assay are described for measuring Factor XIII levels in human plasma by measuring the incorporation of C14-putrescine, at close to saturating concentrations, into casein." @default.
- W2023354858 created "2016-06-24" @default.
- W2023354858 creator A5005235083 @default.
- W2023354858 creator A5023311552 @default.
- W2023354858 creator A5088076342 @default.
- W2023354858 date "1972-12-01" @default.
- W2023354858 modified "2023-10-06" @default.
- W2023354858 title "A filter paper assay for transamidating enzymes using radioactive amine substrates" @default.
- W2023354858 cites W1550179364 @default.
- W2023354858 cites W1550297247 @default.
- W2023354858 cites W1554807651 @default.
- W2023354858 cites W17405798 @default.
- W2023354858 cites W1841822105 @default.
- W2023354858 cites W2001866120 @default.
- W2023354858 cites W2004639360 @default.
- W2023354858 cites W2011275596 @default.
- W2023354858 cites W2054979333 @default.
- W2023354858 cites W2064804771 @default.
- W2023354858 cites W2071756117 @default.
- W2023354858 cites W2086541369 @default.
- W2023354858 cites W2089334467 @default.
- W2023354858 cites W2095204534 @default.
- W2023354858 doi "https://doi.org/10.1016/0003-2697(72)90074-7" @default.
- W2023354858 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4674979" @default.
- W2023354858 hasPublicationYear "1972" @default.
- W2023354858 type Work @default.
- W2023354858 sameAs 2023354858 @default.
- W2023354858 citedByCount "298" @default.
- W2023354858 countsByYear W20233548582012 @default.
- W2023354858 countsByYear W20233548582013 @default.
- W2023354858 countsByYear W20233548582014 @default.
- W2023354858 countsByYear W20233548582016 @default.
- W2023354858 countsByYear W20233548582017 @default.
- W2023354858 countsByYear W20233548582018 @default.
- W2023354858 countsByYear W20233548582023 @default.
- W2023354858 crossrefType "journal-article" @default.
- W2023354858 hasAuthorship W2023354858A5005235083 @default.
- W2023354858 hasAuthorship W2023354858A5023311552 @default.
- W2023354858 hasAuthorship W2023354858A5088076342 @default.
- W2023354858 hasConcept C131779359 @default.
- W2023354858 hasConcept C178498320 @default.
- W2023354858 hasConcept C178790620 @default.
- W2023354858 hasConcept C181199279 @default.
- W2023354858 hasConcept C185592680 @default.
- W2023354858 hasConcept C2776142715 @default.
- W2023354858 hasConcept C2777167505 @default.
- W2023354858 hasConcept C28212737 @default.
- W2023354858 hasConcept C40875361 @default.
- W2023354858 hasConcept C43617362 @default.
- W2023354858 hasConcept C55493867 @default.
- W2023354858 hasConcept C64915919 @default.
- W2023354858 hasConceptScore W2023354858C131779359 @default.
- W2023354858 hasConceptScore W2023354858C178498320 @default.
- W2023354858 hasConceptScore W2023354858C178790620 @default.
- W2023354858 hasConceptScore W2023354858C181199279 @default.
- W2023354858 hasConceptScore W2023354858C185592680 @default.
- W2023354858 hasConceptScore W2023354858C2776142715 @default.
- W2023354858 hasConceptScore W2023354858C2777167505 @default.
- W2023354858 hasConceptScore W2023354858C28212737 @default.
- W2023354858 hasConceptScore W2023354858C40875361 @default.
- W2023354858 hasConceptScore W2023354858C43617362 @default.
- W2023354858 hasConceptScore W2023354858C55493867 @default.
- W2023354858 hasConceptScore W2023354858C64915919 @default.
- W2023354858 hasIssue "2" @default.
- W2023354858 hasLocation W20233548581 @default.
- W2023354858 hasLocation W20233548582 @default.
- W2023354858 hasOpenAccess W2023354858 @default.
- W2023354858 hasPrimaryLocation W20233548581 @default.
- W2023354858 hasRelatedWork W1987755785 @default.
- W2023354858 hasRelatedWork W1992881709 @default.
- W2023354858 hasRelatedWork W2023354858 @default.
- W2023354858 hasRelatedWork W2036727846 @default.
- W2023354858 hasRelatedWork W2048712913 @default.
- W2023354858 hasRelatedWork W2072922649 @default.
- W2023354858 hasRelatedWork W2076111736 @default.
- W2023354858 hasRelatedWork W2082705642 @default.
- W2023354858 hasRelatedWork W2330109456 @default.
- W2023354858 hasRelatedWork W4231501941 @default.
- W2023354858 hasVolume "50" @default.
- W2023354858 isParatext "false" @default.
- W2023354858 isRetracted "false" @default.
- W2023354858 magId "2023354858" @default.
- W2023354858 workType "article" @default.