Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023357032> ?p ?o ?g. }
- W2023357032 endingPage "3256" @default.
- W2023357032 startingPage "3246" @default.
- W2023357032 abstract "Calpains are a family of calcium‐dependent cysteine proteases involved in a variety of cellular functions. Two isoforms, m‐calpain and μ‐calpain, have been implicated in cell migration. However, since conventional inhibitors used for the studies of the functions of these enzymes lack specificity, the individual physiological function and biochemical mechanism of these two isoforms, especially μ‐calpain, are not clear. In contrast, RNA interference has the potential to allow a sequence‐specific destruction of target RNA for functional assay of gene of interest. In the present study, we found that small interfering RNAs‐mediated knockdown of μ‐calpain expression in MCF‐7 cells that do not express m‐Calpain led to a reduction of cell migration. This isoform‐specific function of μ‐calpain was further confirmed by the rescue experiment as overexpression of μ‐calpain but not m‐calpain could restore the cell migration rate. Knockdown of μ‐calpain also altered cell morphology with increased filopodial projections and a highly elongated tail that seemed to prevent cell spreading and migration with reduced rear detachment ability. Furthermore, knockdown of μ‐calpain decreased the proteolytic products of filamin and talin, which were specifically rescued by overexpression of μ‐calpain but not m‐calpain, suggesting that their proteolysis could be one of the key mechanisms by which μ‐calpain regulates cell migration." @default.
- W2023357032 created "2016-06-24" @default.
- W2023357032 creator A5010684771 @default.
- W2023357032 creator A5016102256 @default.
- W2023357032 creator A5027995006 @default.
- W2023357032 creator A5040552215 @default.
- W2023357032 creator A5062422333 @default.
- W2023357032 creator A5066050216 @default.
- W2023357032 date "2006-05-06" @default.
- W2023357032 modified "2023-10-13" @default.
- W2023357032 title "Functional dissection of human protease μ-calpain in cell migration using RNAi" @default.
- W2023357032 cites W1492306774 @default.
- W2023357032 cites W1569251007 @default.
- W2023357032 cites W1758246291 @default.
- W2023357032 cites W1964270286 @default.
- W2023357032 cites W1968815565 @default.
- W2023357032 cites W1969781490 @default.
- W2023357032 cites W1971010898 @default.
- W2023357032 cites W1978742420 @default.
- W2023357032 cites W1979345371 @default.
- W2023357032 cites W1988720723 @default.
- W2023357032 cites W1993312924 @default.
- W2023357032 cites W1996840228 @default.
- W2023357032 cites W2002923380 @default.
- W2023357032 cites W2020472510 @default.
- W2023357032 cites W2021528141 @default.
- W2023357032 cites W2022857511 @default.
- W2023357032 cites W2040948261 @default.
- W2023357032 cites W2043305034 @default.
- W2023357032 cites W2045631079 @default.
- W2023357032 cites W2047359860 @default.
- W2023357032 cites W2054319300 @default.
- W2023357032 cites W2065411998 @default.
- W2023357032 cites W2071596792 @default.
- W2023357032 cites W2081040018 @default.
- W2023357032 cites W2085414692 @default.
- W2023357032 cites W2087140031 @default.
- W2023357032 cites W2088108760 @default.
- W2023357032 cites W2092096186 @default.
- W2023357032 cites W2099253537 @default.
- W2023357032 cites W2107070633 @default.
- W2023357032 cites W2107508122 @default.
- W2023357032 cites W2111853402 @default.
- W2023357032 cites W2134225748 @default.
- W2023357032 cites W2140061120 @default.
- W2023357032 cites W2151078743 @default.
- W2023357032 cites W2154561490 @default.
- W2023357032 cites W2166802510 @default.
- W2023357032 cites W2167307101 @default.
- W2023357032 cites W2169436026 @default.
- W2023357032 cites W2267906346 @default.
- W2023357032 cites W4248206010 @default.
- W2023357032 cites W4256150107 @default.
- W2023357032 doi "https://doi.org/10.1016/j.febslet.2006.05.003" @default.
- W2023357032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16697376" @default.
- W2023357032 hasPublicationYear "2006" @default.
- W2023357032 type Work @default.
- W2023357032 sameAs 2023357032 @default.
- W2023357032 citedByCount "17" @default.
- W2023357032 countsByYear W20233570322012 @default.
- W2023357032 countsByYear W20233570322013 @default.
- W2023357032 countsByYear W20233570322016 @default.
- W2023357032 countsByYear W20233570322018 @default.
- W2023357032 countsByYear W20233570322021 @default.
- W2023357032 crossrefType "journal-article" @default.
- W2023357032 hasAuthorship W2023357032A5010684771 @default.
- W2023357032 hasAuthorship W2023357032A5016102256 @default.
- W2023357032 hasAuthorship W2023357032A5027995006 @default.
- W2023357032 hasAuthorship W2023357032A5040552215 @default.
- W2023357032 hasAuthorship W2023357032A5062422333 @default.
- W2023357032 hasAuthorship W2023357032A5066050216 @default.
- W2023357032 hasBestOaLocation W20233570321 @default.
- W2023357032 hasConcept C104317684 @default.
- W2023357032 hasConcept C137738243 @default.
- W2023357032 hasConcept C142669718 @default.
- W2023357032 hasConcept C1491633281 @default.
- W2023357032 hasConcept C166703698 @default.
- W2023357032 hasConcept C173396325 @default.
- W2023357032 hasConcept C181199279 @default.
- W2023357032 hasConcept C182220744 @default.
- W2023357032 hasConcept C185592680 @default.
- W2023357032 hasConcept C20563397 @default.
- W2023357032 hasConcept C2776714187 @default.
- W2023357032 hasConcept C2777283782 @default.
- W2023357032 hasConcept C2781307694 @default.
- W2023357032 hasConcept C53345823 @default.
- W2023357032 hasConcept C55493867 @default.
- W2023357032 hasConcept C56928146 @default.
- W2023357032 hasConcept C67705224 @default.
- W2023357032 hasConcept C86803240 @default.
- W2023357032 hasConcept C95444343 @default.
- W2023357032 hasConceptScore W2023357032C104317684 @default.
- W2023357032 hasConceptScore W2023357032C137738243 @default.
- W2023357032 hasConceptScore W2023357032C142669718 @default.
- W2023357032 hasConceptScore W2023357032C1491633281 @default.
- W2023357032 hasConceptScore W2023357032C166703698 @default.
- W2023357032 hasConceptScore W2023357032C173396325 @default.
- W2023357032 hasConceptScore W2023357032C181199279 @default.