Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023358213> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2023358213 abstract "Antisense oligonucleotides that selectively inhibit gene expression are a genetic approach for disease treatment and prevention. However, their use as therapeutic agents is complicated by their low rate of transport across cellular membranes and their sequestration within endocytic-like vesicles. We report that the adenovirus type-2 penton base protein modified to include the fusogenic peptide of the influenza virus hemagglutinin protein is a non-replicating vector (designated UTARVE) that improves delivery of antisense oligonucleotides. Approximately 10-18% of the input vector was internalized by A549 and HeLa cells as determined by immunoblotting. It was cleared by proteolysis within 48 h. The vector had endosome disruptive potential as evidenced by erythrocyte lysis activity at low pH and a primarily diffuse cytoplasmic distribution in treated cells. Despite concentration and time-dependent cell detachment, UTARVE was not cytotoxic in the dye release assay. We used R1T1, an antisense oligonucleotide that inhibits expression of the multifunctional herpes simplex virus type-2 (HSV-2) R1 protein, HSV-2 growth and the proliferation of R1 PK transformed cells to examine vector-mediated delivery. Conjugated FITC-labeled R1T1 was rapidly (15-30 min) internalized by all cells treated at low (80 nM) concentration and the oligomer was intracellularly dissociated from the vector. This compares to 65-83% of cells internalizing the unconjugated R1T1 when treated for 24 h. In antiviral assays, the IC50 and time required to inhibit HSV-2 growth were significantly lower for the conjugated (2 nM; 30 min) as compared to unconjugated (100 nM; 24 h) R1T1. The data indicate that the bioavailability and biological activity of R1T1 were significantly increased by its delivery with UTARVE." @default.
- W2023358213 created "2016-06-24" @default.
- W2023358213 creator A5004317823 @default.
- W2023358213 creator A5080476954 @default.
- W2023358213 creator A5085388835 @default.
- W2023358213 date "2000-10-01" @default.
- W2023358213 modified "2023-09-27" @default.
- W2023358213 title "Modified adenovirus penton base protein (UTARVE) as a non-replicating vector for delivery of antisense oligonucleotides with antiviral and/or antineoplastic activity." @default.
- W2023358213 doi "https://doi.org/10.3892/ijo.17.4.841" @default.
- W2023358213 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10995900" @default.
- W2023358213 hasPublicationYear "2000" @default.
- W2023358213 type Work @default.
- W2023358213 sameAs 2023358213 @default.
- W2023358213 citedByCount "1" @default.
- W2023358213 crossrefType "journal-article" @default.
- W2023358213 hasAuthorship W2023358213A5004317823 @default.
- W2023358213 hasAuthorship W2023358213A5080476954 @default.
- W2023358213 hasAuthorship W2023358213A5085388835 @default.
- W2023358213 hasConcept C104317684 @default.
- W2023358213 hasConcept C129312508 @default.
- W2023358213 hasConcept C135983454 @default.
- W2023358213 hasConcept C1491633281 @default.
- W2023358213 hasConcept C153911025 @default.
- W2023358213 hasConcept C159047783 @default.
- W2023358213 hasConcept C2522874641 @default.
- W2023358213 hasConcept C2777366897 @default.
- W2023358213 hasConcept C2781196997 @default.
- W2023358213 hasConcept C32470452 @default.
- W2023358213 hasConcept C40767141 @default.
- W2023358213 hasConcept C54009773 @default.
- W2023358213 hasConcept C54355233 @default.
- W2023358213 hasConcept C552990157 @default.
- W2023358213 hasConcept C55493867 @default.
- W2023358213 hasConcept C81885089 @default.
- W2023358213 hasConcept C86803240 @default.
- W2023358213 hasConceptScore W2023358213C104317684 @default.
- W2023358213 hasConceptScore W2023358213C129312508 @default.
- W2023358213 hasConceptScore W2023358213C135983454 @default.
- W2023358213 hasConceptScore W2023358213C1491633281 @default.
- W2023358213 hasConceptScore W2023358213C153911025 @default.
- W2023358213 hasConceptScore W2023358213C159047783 @default.
- W2023358213 hasConceptScore W2023358213C2522874641 @default.
- W2023358213 hasConceptScore W2023358213C2777366897 @default.
- W2023358213 hasConceptScore W2023358213C2781196997 @default.
- W2023358213 hasConceptScore W2023358213C32470452 @default.
- W2023358213 hasConceptScore W2023358213C40767141 @default.
- W2023358213 hasConceptScore W2023358213C54009773 @default.
- W2023358213 hasConceptScore W2023358213C54355233 @default.
- W2023358213 hasConceptScore W2023358213C552990157 @default.
- W2023358213 hasConceptScore W2023358213C55493867 @default.
- W2023358213 hasConceptScore W2023358213C81885089 @default.
- W2023358213 hasConceptScore W2023358213C86803240 @default.
- W2023358213 hasLocation W20233582131 @default.
- W2023358213 hasLocation W20233582132 @default.
- W2023358213 hasOpenAccess W2023358213 @default.
- W2023358213 hasPrimaryLocation W20233582131 @default.
- W2023358213 hasRelatedWork W1533129085 @default.
- W2023358213 hasRelatedWork W1544165714 @default.
- W2023358213 hasRelatedWork W1572806013 @default.
- W2023358213 hasRelatedWork W1592308507 @default.
- W2023358213 hasRelatedWork W1911803680 @default.
- W2023358213 hasRelatedWork W1998071815 @default.
- W2023358213 hasRelatedWork W2006149037 @default.
- W2023358213 hasRelatedWork W2039546605 @default.
- W2023358213 hasRelatedWork W2043414269 @default.
- W2023358213 hasRelatedWork W2057287106 @default.
- W2023358213 hasRelatedWork W2072409578 @default.
- W2023358213 hasRelatedWork W2105812558 @default.
- W2023358213 hasRelatedWork W2110321421 @default.
- W2023358213 hasRelatedWork W2111764254 @default.
- W2023358213 hasRelatedWork W2116891085 @default.
- W2023358213 hasRelatedWork W2145275814 @default.
- W2023358213 hasRelatedWork W2162803403 @default.
- W2023358213 hasRelatedWork W2171811119 @default.
- W2023358213 hasRelatedWork W2335105539 @default.
- W2023358213 hasRelatedWork W2408522910 @default.
- W2023358213 isParatext "false" @default.
- W2023358213 isRetracted "false" @default.
- W2023358213 magId "2023358213" @default.
- W2023358213 workType "article" @default.