FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023358788> ?p ?o ?g. }

• W2023358788 endingPage "6989" @default.
• W2023358788 startingPage "6980" @default.
• W2023358788 abstract "Phosphorylation of the TNF-alpha receptor TNF-R1 has been shown to differentially regulate receptor signaling and function and promote changes in its subcellular localization. Previous studies have shown that p42(mapk/erk2) phosphorylates Ser and Thr residues (T236, S240, S244, and S270) in the membrane proximal region of TNF-R1 and that mutation of these residues to Glu and Asp residues (TNF-R1.4D/E) mimics the effect of phosphorylation on receptor signaling and localization. In the present study, we investigated whether the initial phosphorylation of these residues by p42(mapk/erk2) promotes hierarchical phosphorylation of additional sites within the cytoplasmic domain of TNF-R1. This question was addressed by investigating the ability of the TNF-R1.4D/E mutant receptor to be phosphorylated in in vitro kinase assays using GST-mutant cytoplasmic domain fusion proteins as substrates and in intact cells following mutant receptor expression. In addition, we determined the location of the additional phosphorylation sites. Incubation of Sepharose bead-bound GST-TNF-R1(207)(-)(425).4D/E fusion protein with lysates containing activated p42(mapk/erk2) led to the phosphorylation of Ser and Thr residues in addition to the previously defined sites at T236, S240, S244, and S270. Deletional mutagenesis localized these residues to a stretch of 14 amino acids that encompasses three basic Pro-directed ([S/T]P) kinase consensus sequences located between residues S256 and T267. Point mutagenesis of T257, S262, and T267 to Ala residues indicated that these sites are targets of phosphorylation by p42(mapk/)(erk2). These findings support the conclusion that p42(mapk/erk2) promotes extensive phosphorylation of the membrane proximal region in a hierarchical fashion at both consensus and nonconsensus ERK-phosphorylation sites." @default.
• W2023358788 created "2016-06-24" @default.
• W2023358788 creator A5008453489 @default.
• W2023358788 creator A5014703126 @default.
• W2023358788 creator A5031193757 @default.
• W2023358788 creator A5070110944 @default.
• W2023358788 date "2005-04-13" @default.
• W2023358788 modified "2023-09-26" @default.
• W2023358788 title "Hierarchical Phosphorylation of the TNF-α Receptor, TNF-R1, by p42^m^apk/^e^rk at Basic Pro-Directed Kinase Sites" @default.
• W2023358788 cites W1587453136 @default.
• W2023358788 cites W1968160256 @default.
• W2023358788 cites W1984142065 @default.
• W2023358788 cites W1997689219 @default.
• W2023358788 cites W2017973051 @default.
• W2023358788 doi "https://doi.org/10.1021/bi050058w" @default.
• W2023358788 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15865443" @default.
• W2023358788 hasPublicationYear "2005" @default.
• W2023358788 type Work @default.
• W2023358788 sameAs 2023358788 @default.
• W2023358788 citedByCount "3" @default.
• W2023358788 countsByYear W20233587882014 @default.
• W2023358788 countsByYear W20233587882019 @default.
• W2023358788 crossrefType "journal-article" @default.
• W2023358788 hasAuthorship W2023358788A5008453489 @default.
• W2023358788 hasAuthorship W2023358788A5014703126 @default.
• W2023358788 hasAuthorship W2023358788A5031193757 @default.
• W2023358788 hasAuthorship W2023358788A5070110944 @default.
• W2023358788 hasConcept C104317684 @default.
• W2023358788 hasConcept C11960822 @default.
• W2023358788 hasConcept C123894998 @default.
• W2023358788 hasConcept C143065580 @default.
• W2023358788 hasConcept C153911025 @default.
• W2023358788 hasConcept C16318435 @default.
• W2023358788 hasConcept C170493617 @default.
• W2023358788 hasConcept C184235292 @default.
• W2023358788 hasConcept C185592680 @default.
• W2023358788 hasConcept C40767141 @default.
• W2023358788 hasConcept C55493867 @default.
• W2023358788 hasConcept C57074206 @default.
• W2023358788 hasConcept C86803240 @default.
• W2023358788 hasConcept C95444343 @default.
• W2023358788 hasConcept C97029542 @default.
• W2023358788 hasConceptScore W2023358788C104317684 @default.
• W2023358788 hasConceptScore W2023358788C11960822 @default.
• W2023358788 hasConceptScore W2023358788C123894998 @default.
• W2023358788 hasConceptScore W2023358788C143065580 @default.
• W2023358788 hasConceptScore W2023358788C153911025 @default.
• W2023358788 hasConceptScore W2023358788C16318435 @default.
• W2023358788 hasConceptScore W2023358788C170493617 @default.
• W2023358788 hasConceptScore W2023358788C184235292 @default.
• W2023358788 hasConceptScore W2023358788C185592680 @default.
• W2023358788 hasConceptScore W2023358788C40767141 @default.
• W2023358788 hasConceptScore W2023358788C55493867 @default.
• W2023358788 hasConceptScore W2023358788C57074206 @default.
• W2023358788 hasConceptScore W2023358788C86803240 @default.
• W2023358788 hasConceptScore W2023358788C95444343 @default.
• W2023358788 hasConceptScore W2023358788C97029542 @default.
• W2023358788 hasIssue "18" @default.
• W2023358788 hasLocation W20233587881 @default.
• W2023358788 hasLocation W20233587882 @default.
• W2023358788 hasOpenAccess W2023358788 @default.
• W2023358788 hasPrimaryLocation W20233587881 @default.
• W2023358788 hasRelatedWork W1990580490 @default.
• W2023358788 hasRelatedWork W2010932108 @default.
• W2023358788 hasRelatedWork W2015725807 @default.
• W2023358788 hasRelatedWork W2081246372 @default.
• W2023358788 hasRelatedWork W2091587849 @default.
• W2023358788 hasRelatedWork W2095169768 @default.
• W2023358788 hasRelatedWork W2287845648 @default.
• W2023358788 hasRelatedWork W2805781019 @default.
• W2023358788 hasRelatedWork W2978781268 @default.
• W2023358788 hasRelatedWork W3156080906 @default.
• W2023358788 hasVolume "44" @default.
• W2023358788 isParatext "false" @default.
• W2023358788 isRetracted "false" @default.
• W2023358788 magId "2023358788" @default.
• W2023358788 workType "article" @default.