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- W2023367243 abstract "The normal cellular c-src gene encodes a 60-kDa nonreceptor tyrosine-specific protein kinase (pp60c-src) that is homologous to the transforming protein of Rous sarcoma virus. pp60c-src shows a strong evolutionary conservation among Metazoa, but its physiological role has not yet been identified. Here we review methods employed to characterize the localization of pp60c-src in the developing nervous system and in growth cones. We describe how to identify potential in vivo substrates of pp60c-src. Nerve growth cones isolated by subcellular fractionation prove to be a powerful tool for investigating modulations of tyrosine kinase activity by cell adhesion molecules, extracellular matrix proteins, or second-messenger effectors. Kinase activity can be analyzed by immune complex kinase assays or in endogenous phosphorylation assays within the isolated growth cone membrane." @default.
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- W2023367243 date "1992-12-01" @default.
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- W2023367243 title "Tyrosine kinase pp60c-src in Nerve growth cones and developing brain: Topographical and functional analysis" @default.
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- W2023367243 doi "https://doi.org/10.1016/1058-6741(92)90032-s" @default.
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