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• W2023369053 abstract "FEBS LettersVolume 292, Issue 1-2 p. 48-52 Full‐length articleFree Access Expression of the pea genePsMTA inE. coli Metal‐binding properties of the expressed protein First published: November 04, 1991 https://doi.org/10.1016/0014-5793(91)80831-MCitations: 97AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The pea (Pisum sativum L) genePsMTA has an ORF encoding a predicted protein with sequence similarity to class I metallothioneins (MTs). To examine the metal‐binding properties of the PsMTA protein it has been expressed inE. coli as a car☐yterminal extension of glutathione‐S‐transferase (GST). Metal ions were associated with the expressed protein when purified from lysates ofE. coli grown in metal supplemented media. The pH of half‐dissociation of Zn, Cd and Cu ions from the recombinant fusion protein was determined to be 5.35, 3.95 and 1.45 respectively, compared with equivalent estimates of 4.50, 3.00 and 1.80 for equine renal MT. References 1 Lane B. Kajioka R. Kennedy T. Biochem. Cell Biol. 65,(1987), 1001– 1005. 2 Kaägi J.H.R. Schaäffer A. Biochemistry 27,(1988), 8509– 8515. 3 Evans I.M. Gatehouse L.N. Gatehouse J.A. Robinson N.J. Croy R.R.D. FEDS Lett. 262,(1990), 29– 32. 4 de Miranda J.R. Thomas M.A. Thurman D.A. Tomsett A.B. FEDS Lett. 260,(1990), 277– 280. 5 Murooka Y. Nagaoka T. Appl. Environ. Microbiol. 53,(1987), 204– 207. 6 Romeyer F.M. Jacobs F.A. Masson L. Hanna Z. Brousseau R. J. Biotechnol. 8,(1988), 207– 220. 7 Jacobs F.A. Romeyer F.M. Beauchemin M. Brousseau R. Gene 83,(1989), 95– 103. 8 Kille P. Stephens P. Cryer A. Kay J. Biochim. Biophys. Acta 1048,(1990), 178– 186. 9 Silar P. Wegnez M. FEBS Lett. 269,(1990), 273– 276. 10 Romeyer F.M. Jacobs F.A. Brousseau R. Appl. Environ. Microbiol. 56,(1990), 2748– 2754. 11 Evans I.M. Swinhoe R. Gatehouse L.N. Gatehouse J.A. Boulter D. Mol. Gen. Genet. 214,(1988), 153– 157. 12 Robinson N.J. Gupta A. Fordham-Skelton A.P. Croy R.R.D. Whitton B.A. Huckle J.W. Proc. Roy. Soc. London Ser. B 242,(1990), 241– 247. 13 Smith D.B. Johnson K.S. Gene 67,(1988), 31– 40. 14 Laemmli U.K. Nature 277,(1970), 680– 685. 15 Nagai K. Thorgerson H.C. Nature 309,(1984), 810– 812. 16 Gearing D.P. Nicola N.A. Metcalf D. Foote S. Wilson T.A. Crough N.M. Williams R.L. Bio/Technology 7,(1989), 1157– 1161. 17 Vasak M. Armitage I. Environ. Health Perspect. 65,(1986), 215– 216. 18 Kaägi J.H.R. Vallee B.L. J. Biol. Chem. 235,(1960), 3460– 3465. 19 Kaägi J.H.R. Vallee B.L. J. Biol. Chem. 236,(1961), 2535– 2542. 20 Grill E. Winnaker E-L. Zenk M.H. Science 230,(1985), 574– 576. 21 Jackson P.J. Unkefer C.J. Doolen J.A. Watt K. Robinson N.J. Proc. Natl. Acad. Sci. USA 84,(1987), 6619– 6623. 22 Kondo N. Isobe M. Imai K. Goto T. Agric. Biol. Chem. 49,(1985), 71– 83. 23 Rauser W.E. Annu. Rev. Biochem. 59,(1990), 61– 86. 24 Steffens J.C. Annu. Rev. Plant Physiol. Plant Mol. Biol. 41,(1990), 553– 575. 25 Robinson N.J. Jackson P.J. Physiol. Plant. 67,(1986), 499– 506. 26 Murasugi A. Wada C. Hayashi Y. J. Biochem. 93,(1983), 661– 664. 27 Verkleij J.A.C. Koevoets P. Van 't Reit J. Bank R. Nijdam Y. Ernst W.H.O. Plant Cell. Environ. 13,(1990), 913– 921. 28 Reese R.N. Mehra R.K. Tarbet E.B. Winge D.R. J. Biol. Chem. 263,(1988), 4186– 4192. 29 Reese R.N. Wagner G.J. Biochem. J. 241,(1987), 641– 647. Citing Literature Volume292, Issue1-2November 04, 1991Pages 48-52 ReferencesRelatedInformation" @default.
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• W2023369053 title "Expression of the pea gene<i>PsMT_A</i>in<i>E. coli</i>Metal&#x2010;binding properties of the expressed protein" @default.
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