Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023370085> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2023370085 abstract "In order to implement three-color surface immunofluorescence on a single laser flow cytometer, we combined a new fluorescent tandem conjugate (TC, R-phycoerythrin plus Texas red, available as DuoCHROME) with fluorescein (FITC)- and phycoerythrin (PE)-labelled monoclonal antibodies for the simultaneous detection of three antigens on individual lymphoid cells. Considerable amounts of fluorescence leaked from the PE component of the TC complex into the PE detector, which necessitated compensation of the PE signal. Single and double stainings of peripheral lymphocytes with FITC-, PE- and/or TC-labelled antibodies revealed that the individual labels all identified populations of similar size, and that subsets of T cells could be properly and accurately detected with TC as one label. Triple staining was used to study phenotypically activated HLA-DR+ cells within functionally important subsets of CD4+ T cells, i.e., CD45R+ CD4+ (naive, 'suppressor inducer' T) and UCHL1+ CD4+ (memory, 'helper' T) cells. In the blood of healthy subjects as well as patients with primary biliary cirrhosis, CD4+ UCHL1+ (i.e., memory) T cells were significantly more activated than CD4+ UCHL1- cells. The reciprocal CD45R+ CD4+ (naive) T subset expressed HLA-DR to a much lesser extent. In patient samples, this difference in CD4+ T subset activation was more pronounced than among normal lymphocytes. The described triple immunofluorescence staining technique facilitates true multicolor analysis of individual cells on a single laser flow cytometer. Such multiparameter investigations may prove to be important for the further understanding of the phenotypic and functional diversity of immunocompetent cells." @default.
- W2023370085 created "2016-06-24" @default.
- W2023370085 creator A5069734579 @default.
- W2023370085 creator A5072234235 @default.
- W2023370085 creator A5078122014 @default.
- W2023370085 date "1990-01-01" @default.
- W2023370085 modified "2023-09-26" @default.
- W2023370085 title "Single laser flow cytometric detection of lymphocytes binding three antibodies labelled with fluorescein, phycoerythrin and a novel tandem fluorochrome conjugate" @default.
- W2023370085 cites W1988791393 @default.
- W2023370085 cites W2000172498 @default.
- W2023370085 cites W2008441599 @default.
- W2023370085 cites W2012204136 @default.
- W2023370085 cites W2024287229 @default.
- W2023370085 cites W2026527900 @default.
- W2023370085 cites W2032117360 @default.
- W2023370085 cites W2046898329 @default.
- W2023370085 cites W2064169983 @default.
- W2023370085 cites W2075815217 @default.
- W2023370085 cites W2077540752 @default.
- W2023370085 cites W2077839946 @default.
- W2023370085 cites W2124568244 @default.
- W2023370085 cites W2167936032 @default.
- W2023370085 doi "https://doi.org/10.1016/0022-1759(90)90013-l" @default.
- W2023370085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2137494" @default.
- W2023370085 hasPublicationYear "1990" @default.
- W2023370085 type Work @default.
- W2023370085 sameAs 2023370085 @default.
- W2023370085 citedByCount "26" @default.
- W2023370085 countsByYear W20233700852012 @default.
- W2023370085 crossrefType "journal-article" @default.
- W2023370085 hasAuthorship W2023370085A5069734579 @default.
- W2023370085 hasAuthorship W2023370085A5072234235 @default.
- W2023370085 hasAuthorship W2023370085A5078122014 @default.
- W2023370085 hasConcept C121332964 @default.
- W2023370085 hasConcept C134306372 @default.
- W2023370085 hasConcept C137243819 @default.
- W2023370085 hasConcept C147483822 @default.
- W2023370085 hasConcept C153911025 @default.
- W2023370085 hasConcept C159654299 @default.
- W2023370085 hasConcept C185592680 @default.
- W2023370085 hasConcept C197336794 @default.
- W2023370085 hasConcept C203014093 @default.
- W2023370085 hasConcept C2778015030 @default.
- W2023370085 hasConcept C2778236600 @default.
- W2023370085 hasConcept C2992881475 @default.
- W2023370085 hasConcept C33923547 @default.
- W2023370085 hasConcept C542903549 @default.
- W2023370085 hasConcept C54355233 @default.
- W2023370085 hasConcept C553184892 @default.
- W2023370085 hasConcept C62520636 @default.
- W2023370085 hasConcept C74864618 @default.
- W2023370085 hasConcept C86803240 @default.
- W2023370085 hasConcept C91881484 @default.
- W2023370085 hasConceptScore W2023370085C121332964 @default.
- W2023370085 hasConceptScore W2023370085C134306372 @default.
- W2023370085 hasConceptScore W2023370085C137243819 @default.
- W2023370085 hasConceptScore W2023370085C147483822 @default.
- W2023370085 hasConceptScore W2023370085C153911025 @default.
- W2023370085 hasConceptScore W2023370085C159654299 @default.
- W2023370085 hasConceptScore W2023370085C185592680 @default.
- W2023370085 hasConceptScore W2023370085C197336794 @default.
- W2023370085 hasConceptScore W2023370085C203014093 @default.
- W2023370085 hasConceptScore W2023370085C2778015030 @default.
- W2023370085 hasConceptScore W2023370085C2778236600 @default.
- W2023370085 hasConceptScore W2023370085C2992881475 @default.
- W2023370085 hasConceptScore W2023370085C33923547 @default.
- W2023370085 hasConceptScore W2023370085C542903549 @default.
- W2023370085 hasConceptScore W2023370085C54355233 @default.
- W2023370085 hasConceptScore W2023370085C553184892 @default.
- W2023370085 hasConceptScore W2023370085C62520636 @default.
- W2023370085 hasConceptScore W2023370085C74864618 @default.
- W2023370085 hasConceptScore W2023370085C86803240 @default.
- W2023370085 hasConceptScore W2023370085C91881484 @default.
- W2023370085 hasLocation W20233700851 @default.
- W2023370085 hasLocation W20233700852 @default.
- W2023370085 hasOpenAccess W2023370085 @default.
- W2023370085 hasPrimaryLocation W20233700851 @default.
- W2023370085 hasRelatedWork W1989289676 @default.
- W2023370085 hasRelatedWork W2023775957 @default.
- W2023370085 hasRelatedWork W2037509311 @default.
- W2023370085 hasRelatedWork W2043206546 @default.
- W2023370085 hasRelatedWork W2073262237 @default.
- W2023370085 hasRelatedWork W2094194110 @default.
- W2023370085 hasRelatedWork W2196101296 @default.
- W2023370085 hasRelatedWork W2410111069 @default.
- W2023370085 hasRelatedWork W4250557310 @default.
- W2023370085 hasRelatedWork W89204553 @default.
- W2023370085 isParatext "false" @default.
- W2023370085 isRetracted "false" @default.
- W2023370085 magId "2023370085" @default.
- W2023370085 workType "article" @default.