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• W2023373964 startingPage "9486" @default.
• W2023373964 abstract "ABSTRACT The coronavirus nucleocapsid (N) protein plays a multifunctional role in the virus life cycle, from regulation of replication and transcription and genome packaging to modulation of host cell processes. These functions are likely to be facilitated by interactions with host cell proteins. The potential interactome of the infectious bronchitis virus (IBV) N protein was mapped using stable isotope labeling with amino acids in cell culture (SILAC) coupled to a green fluorescent protein-nanotrap pulldown methodology and liquid chromatography-tandem mass spectrometry. The addition of the SILAC label allowed discrimination of proteins that were likely to specifically bind to the N protein over background binding. Overall, 142 cellular proteins were selected as potentially binding to the N protein, many as part of larger possible complexes. These included ribosomal proteins, nucleolar proteins, translation initiation factors, helicases, and hnRNPs. The association of selected cellular proteins with IBV N protein was confirmed by immunoblotting, cosedimentation, and confocal microscopy. Further, the localization of selected proteins in IBV-infected cells as well as their activity during virus infection was assessed by small interfering RNA-mediated depletion, demonstrating the functional importance of cellular proteins in the biology of IBV. This interactome not only confirms previous observations made with other coronavirus and IBV N proteins with both overexpressed proteins and infectious virus but also provides novel data that can be exploited to understand the interaction between the virus and the host cell." @default.
• W2023373964 created "2016-06-24" @default.
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• W2023373964 date "2013-09-01" @default.
• W2023373964 modified "2023-10-18" @default.
• W2023373964 title "The Cellular Interactome of the Coronavirus Infectious Bronchitis Virus Nucleocapsid Protein and Functional Implications for Virus Biology" @default.
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• W2023373964 cites W1968561529 @default.
• W2023373964 cites W1978492897 @default.
• W2023373964 cites W1981376913 @default.
• W2023373964 cites W1985103810 @default.
• W2023373964 cites W1986406001 @default.
• W2023373964 cites W1989969147 @default.
• W2023373964 cites W1990649962 @default.
• W2023373964 cites W1992923908 @default.
• W2023373964 cites W1998859166 @default.
• W2023373964 cites W2001039644 @default.
• W2023373964 cites W2002398153 @default.
• W2023373964 cites W2002535283 @default.
• W2023373964 cites W2008042305 @default.
• W2023373964 cites W2008089898 @default.
• W2023373964 cites W2015407064 @default.
• W2023373964 cites W2020142830 @default.
• W2023373964 cites W2029358388 @default.
• W2023373964 cites W2029865102 @default.
• W2023373964 cites W2030364628 @default.
• W2023373964 cites W2032639971 @default.
• W2023373964 cites W2035676489 @default.
• W2023373964 cites W2039183513 @default.
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• W2023373964 cites W2049195530 @default.
• W2023373964 cites W2051810299 @default.
• W2023373964 cites W2056431509 @default.
• W2023373964 cites W2056614343 @default.
• W2023373964 cites W2056636829 @default.
• W2023373964 cites W2057929614 @default.
• W2023373964 cites W2058625821 @default.
• W2023373964 cites W2062962568 @default.
• W2023373964 cites W2066786363 @default.
• W2023373964 cites W2074858838 @default.
• W2023373964 cites W2077283045 @default.
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• W2023373964 cites W2082849856 @default.
• W2023373964 cites W2086182923 @default.
• W2023373964 cites W2088244647 @default.
• W2023373964 cites W2093606554 @default.
• W2023373964 cites W2097698606 @default.
• W2023373964 cites W2104414253 @default.
• W2023373964 cites W2105568807 @default.
• W2023373964 cites W2105924489 @default.
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• W2023373964 cites W2107405224 @default.
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• W2023373964 cites W2111080041 @default.
• W2023373964 cites W2113641724 @default.
• W2023373964 cites W2116803802 @default.
• W2023373964 cites W2127458794 @default.
• W2023373964 cites W2135921282 @default.
• W2023373964 cites W2137467403 @default.
• W2023373964 cites W2139440265 @default.
• W2023373964 cites W2143546506 @default.
• W2023373964 cites W2148636009 @default.
• W2023373964 cites W2150818376 @default.
• W2023373964 cites W2152666867 @default.
• W2023373964 cites W2153233254 @default.
• W2023373964 cites W2156681639 @default.
• W2023373964 cites W2157193236 @default.
• W2023373964 cites W2160108367 @default.
• W2023373964 cites W2162555389 @default.
• W2023373964 cites W2163098777 @default.
• W2023373964 cites W2165253681 @default.
• W2023373964 cites W2165899210 @default.
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• W2023373964 cites W2169825756 @default.
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• W2023373964 cites W2170888162 @default.
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• W2023373964 doi "https://doi.org/10.1128/jvi.00321-13" @default.
• W2023373964 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3754094" @default.
• W2023373964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23637410" @default.
• W2023373964 hasPublicationYear "2013" @default.
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