Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023375991> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2023375991 abstract "The steady-state mRNA levels of the proliferating cell nuclear antigen (PCNA) gene depend on the length of its promoter. A promoter extending from the HpaII restriction site at -210 from the cap site to the cap site itself is very active, while a -45 promoter (AatII restriction site) is very weak. We now show that the sequences between -73 and -45 of the human PCNA promoter contain an enhancer-like sequence that markedly increases the levels of PCNA mRNA. This sequence has characteristics of an enhancer, having an enhancing function also when placed away from the native position in the 5' flanking sequence. The increase in mRNA levels that occurs after serum stimulation, however, is independent of the enhancer. Synthetic promoters were also constructed containing mutations in the -73 to -45 sequence and these mutants completely lost their ability to drive the transcription of a heterologous cDNA. Nuclear proteins were shown to bind to this sequence, both by gel shift and by methylation interference analysis. We conclude that the levels of PCNA mRNA are controlled, in part, by a structure located in the 5' flanking sequence of the gene, but that this enhancer-like structure does not play a role in the serum regulation of the mRNA levels." @default.
- W2023375991 created "2016-06-24" @default.
- W2023375991 creator A5012373469 @default.
- W2023375991 creator A5045704463 @default.
- W2023375991 creator A5062094526 @default.
- W2023375991 creator A5063312933 @default.
- W2023375991 creator A5087771423 @default.
- W2023375991 date "1991-04-01" @default.
- W2023375991 modified "2023-10-14" @default.
- W2023375991 title "Characterization of an enhancer-like structure in the promoter region of the proliferating cell nuclear antigen (PCNA) gene" @default.
- W2023375991 cites W1503849321 @default.
- W2023375991 cites W1509615239 @default.
- W2023375991 cites W1550698494 @default.
- W2023375991 cites W1761872749 @default.
- W2023375991 cites W1846390947 @default.
- W2023375991 cites W1954580706 @default.
- W2023375991 cites W1956097919 @default.
- W2023375991 cites W1993243592 @default.
- W2023375991 cites W1995129870 @default.
- W2023375991 cites W2001230855 @default.
- W2023375991 cites W2007615149 @default.
- W2023375991 cites W2008317513 @default.
- W2023375991 cites W2014669590 @default.
- W2023375991 cites W2017273273 @default.
- W2023375991 cites W2020994399 @default.
- W2023375991 cites W2021345772 @default.
- W2023375991 cites W2029440030 @default.
- W2023375991 cites W2031504305 @default.
- W2023375991 cites W2045590352 @default.
- W2023375991 cites W2051770415 @default.
- W2023375991 cites W2068577315 @default.
- W2023375991 cites W2088272992 @default.
- W2023375991 cites W2112005597 @default.
- W2023375991 cites W2127201025 @default.
- W2023375991 cites W2129419965 @default.
- W2023375991 cites W2134812217 @default.
- W2023375991 cites W4253191292 @default.
- W2023375991 cites W838635636 @default.
- W2023375991 doi "https://doi.org/10.1016/0014-4827(91)90098-f" @default.
- W2023375991 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1672277" @default.
- W2023375991 hasPublicationYear "1991" @default.
- W2023375991 type Work @default.
- W2023375991 sameAs 2023375991 @default.
- W2023375991 citedByCount "18" @default.
- W2023375991 countsByYear W20233759912012 @default.
- W2023375991 crossrefType "journal-article" @default.
- W2023375991 hasAuthorship W2023375991A5012373469 @default.
- W2023375991 hasAuthorship W2023375991A5045704463 @default.
- W2023375991 hasAuthorship W2023375991A5062094526 @default.
- W2023375991 hasAuthorship W2023375991A5063312933 @default.
- W2023375991 hasAuthorship W2023375991A5087771423 @default.
- W2023375991 hasConcept C101762097 @default.
- W2023375991 hasConcept C104317684 @default.
- W2023375991 hasConcept C105580179 @default.
- W2023375991 hasConcept C111936080 @default.
- W2023375991 hasConcept C138885662 @default.
- W2023375991 hasConcept C150194340 @default.
- W2023375991 hasConcept C153911025 @default.
- W2023375991 hasConcept C179926584 @default.
- W2023375991 hasConcept C21592294 @default.
- W2023375991 hasConcept C21784809 @default.
- W2023375991 hasConcept C34688535 @default.
- W2023375991 hasConcept C41895202 @default.
- W2023375991 hasConcept C54355233 @default.
- W2023375991 hasConcept C552990157 @default.
- W2023375991 hasConcept C86803240 @default.
- W2023375991 hasConceptScore W2023375991C101762097 @default.
- W2023375991 hasConceptScore W2023375991C104317684 @default.
- W2023375991 hasConceptScore W2023375991C105580179 @default.
- W2023375991 hasConceptScore W2023375991C111936080 @default.
- W2023375991 hasConceptScore W2023375991C138885662 @default.
- W2023375991 hasConceptScore W2023375991C150194340 @default.
- W2023375991 hasConceptScore W2023375991C153911025 @default.
- W2023375991 hasConceptScore W2023375991C179926584 @default.
- W2023375991 hasConceptScore W2023375991C21592294 @default.
- W2023375991 hasConceptScore W2023375991C21784809 @default.
- W2023375991 hasConceptScore W2023375991C34688535 @default.
- W2023375991 hasConceptScore W2023375991C41895202 @default.
- W2023375991 hasConceptScore W2023375991C54355233 @default.
- W2023375991 hasConceptScore W2023375991C552990157 @default.
- W2023375991 hasConceptScore W2023375991C86803240 @default.
- W2023375991 hasLocation W20233759911 @default.
- W2023375991 hasLocation W20233759912 @default.
- W2023375991 hasOpenAccess W2023375991 @default.
- W2023375991 hasPrimaryLocation W20233759911 @default.
- W2023375991 hasRelatedWork W2023375991 @default.
- W2023375991 hasRelatedWork W2053801949 @default.
- W2023375991 hasRelatedWork W2126201788 @default.
- W2023375991 hasRelatedWork W2126789309 @default.
- W2023375991 hasRelatedWork W2148279650 @default.
- W2023375991 hasRelatedWork W2171567367 @default.
- W2023375991 hasRelatedWork W2615895636 @default.
- W2023375991 hasRelatedWork W4237518504 @default.
- W2023375991 hasRelatedWork W4256377748 @default.
- W2023375991 hasRelatedWork W2114393100 @default.
- W2023375991 isParatext "false" @default.
- W2023375991 isRetracted "false" @default.
- W2023375991 magId "2023375991" @default.
- W2023375991 workType "article" @default.