Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023377544> ?p ?o ?g. }
- W2023377544 endingPage "165" @default.
- W2023377544 startingPage "159" @default.
- W2023377544 abstract "Mouse Ltk− (H-2k) cells that became reactive with anti-H-2d monoclonal antibodies (McAb) after transfection with DNA from H-2d-positive cells were isolated by an indirect panning method. The cells were transfected with DNA from a plasmid containing the selective thymidine kinase (tk) gene in addition to total cell DNA, so that a first selection could be carried out in hypoxanthine/aminopterin/thymidine (HAT) medium. The HAT-selected tk+ transfectants were incubated with anti-H-2d McAb, washed and transferred to dishes coated with purified anti-immunoglobulin (anti-Ig). This 2-step method of panning has the advantage that only the anti-Ig reagent requires purification. Transfected cells clearly reactive with either anti-Ld/Rd or anti-Kd McAb were isolated after only 1 or2 cycles of panning." @default.
- W2023377544 created "2016-06-24" @default.
- W2023377544 creator A5025838132 @default.
- W2023377544 creator A5035256051 @default.
- W2023377544 creator A5059160872 @default.
- W2023377544 date "1985-05-01" @default.
- W2023377544 modified "2023-09-23" @default.
- W2023377544 title "A simple panning method for the selection of cell surface antigen transfectants" @default.
- W2023377544 cites W1497278722 @default.
- W2023377544 cites W1530393315 @default.
- W2023377544 cites W1547913407 @default.
- W2023377544 cites W1584923298 @default.
- W2023377544 cites W1965372133 @default.
- W2023377544 cites W1980914576 @default.
- W2023377544 cites W1982263288 @default.
- W2023377544 cites W1998162329 @default.
- W2023377544 cites W2007262506 @default.
- W2023377544 cites W2008809605 @default.
- W2023377544 cites W2022485234 @default.
- W2023377544 cites W2027411626 @default.
- W2023377544 cites W2033819427 @default.
- W2023377544 cites W2036928923 @default.
- W2023377544 cites W2076599568 @default.
- W2023377544 cites W2080343915 @default.
- W2023377544 cites W2081271027 @default.
- W2023377544 cites W2082418131 @default.
- W2023377544 cites W2121349955 @default.
- W2023377544 cites W2122385641 @default.
- W2023377544 cites W2133508418 @default.
- W2023377544 cites W2142617031 @default.
- W2023377544 cites W2162003018 @default.
- W2023377544 cites W2172009488 @default.
- W2023377544 cites W2412941035 @default.
- W2023377544 doi "https://doi.org/10.1016/0022-1759(85)90402-8" @default.
- W2023377544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2987357" @default.
- W2023377544 hasPublicationYear "1985" @default.
- W2023377544 type Work @default.
- W2023377544 sameAs 2023377544 @default.
- W2023377544 citedByCount "4" @default.
- W2023377544 crossrefType "journal-article" @default.
- W2023377544 hasAuthorship W2023377544A5025838132 @default.
- W2023377544 hasAuthorship W2023377544A5035256051 @default.
- W2023377544 hasAuthorship W2023377544A5059160872 @default.
- W2023377544 hasConcept C104317684 @default.
- W2023377544 hasConcept C108944566 @default.
- W2023377544 hasConcept C124913957 @default.
- W2023377544 hasConcept C147483822 @default.
- W2023377544 hasConcept C151730666 @default.
- W2023377544 hasConcept C15336307 @default.
- W2023377544 hasConcept C153911025 @default.
- W2023377544 hasConcept C159047783 @default.
- W2023377544 hasConcept C159654299 @default.
- W2023377544 hasConcept C185592680 @default.
- W2023377544 hasConcept C203014093 @default.
- W2023377544 hasConcept C22744801 @default.
- W2023377544 hasConcept C2522874641 @default.
- W2023377544 hasConcept C2776147627 @default.
- W2023377544 hasConcept C2777108182 @default.
- W2023377544 hasConcept C2781196997 @default.
- W2023377544 hasConcept C54009773 @default.
- W2023377544 hasConcept C542903549 @default.
- W2023377544 hasConcept C54355233 @default.
- W2023377544 hasConcept C552990157 @default.
- W2023377544 hasConcept C55493867 @default.
- W2023377544 hasConcept C81885089 @default.
- W2023377544 hasConcept C86803240 @default.
- W2023377544 hasConceptScore W2023377544C104317684 @default.
- W2023377544 hasConceptScore W2023377544C108944566 @default.
- W2023377544 hasConceptScore W2023377544C124913957 @default.
- W2023377544 hasConceptScore W2023377544C147483822 @default.
- W2023377544 hasConceptScore W2023377544C151730666 @default.
- W2023377544 hasConceptScore W2023377544C15336307 @default.
- W2023377544 hasConceptScore W2023377544C153911025 @default.
- W2023377544 hasConceptScore W2023377544C159047783 @default.
- W2023377544 hasConceptScore W2023377544C159654299 @default.
- W2023377544 hasConceptScore W2023377544C185592680 @default.
- W2023377544 hasConceptScore W2023377544C203014093 @default.
- W2023377544 hasConceptScore W2023377544C22744801 @default.
- W2023377544 hasConceptScore W2023377544C2522874641 @default.
- W2023377544 hasConceptScore W2023377544C2776147627 @default.
- W2023377544 hasConceptScore W2023377544C2777108182 @default.
- W2023377544 hasConceptScore W2023377544C2781196997 @default.
- W2023377544 hasConceptScore W2023377544C54009773 @default.
- W2023377544 hasConceptScore W2023377544C542903549 @default.
- W2023377544 hasConceptScore W2023377544C54355233 @default.
- W2023377544 hasConceptScore W2023377544C552990157 @default.
- W2023377544 hasConceptScore W2023377544C55493867 @default.
- W2023377544 hasConceptScore W2023377544C81885089 @default.
- W2023377544 hasConceptScore W2023377544C86803240 @default.
- W2023377544 hasIssue "1" @default.
- W2023377544 hasLocation W20233775441 @default.
- W2023377544 hasLocation W20233775442 @default.
- W2023377544 hasOpenAccess W2023377544 @default.
- W2023377544 hasPrimaryLocation W20233775441 @default.
- W2023377544 hasRelatedWork W106631345 @default.
- W2023377544 hasRelatedWork W1970913930 @default.
- W2023377544 hasRelatedWork W1973692620 @default.
- W2023377544 hasRelatedWork W2023377544 @default.