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- W2023379323 abstract "We describe the isolation of a new class 2 restriction endonudease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires mg$ $ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda." @default.
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- W2023379323 date "1976-02-01" @default.
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- W2023379323 title "The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii." @default.
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- W2023379323 doi "https://doi.org/10.1093/nar/3.2.343" @default.
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