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- W2023380421 abstract "An enzyme, cyanase, which breaks down cyanate to CO2 and ammonia has been demonstrated in E. coli. The enzyme is adaptive and is synthesised in the lag as well as the logarithmic growth phase of the cells. Concentrations that inhibit the growth of E. coli permit enzyme synthesis in the lag phase but not in the logarithmic growth phase unless the preliminary growth of the cells occurs in low concentrations of cyanate. The enzyme has been partially purified, and its Km value, pH sensitivity and stability have been described. The synthesis of the enzyme may be inhibited by various nucleic acid antagonists as well as by the inhibition of protein synthesis. Cyanase activity itself cannot be inhibited by KCN, NaF, and only the activity is depressed by KCNS while the yield remains unaffected. The effect of bacteriophage infection on the synthesis of the enzyme has been described, and it has also been shown that simultaneous synthesis of cyanase and β-galactosidase can take place." @default.
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- W2023380421 date "1960-01-01" @default.
- W2023380421 modified "2023-09-27" @default.
- W2023380421 title "The synthesis of the induced enzyme, “cyanase”, in E. coli" @default.
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- W2023380421 doi "https://doi.org/10.1016/0006-3002(60)91605-x" @default.
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