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• W2023385280 abstract "In order to study the mixed lymphocyte culture reactivity of human lymphocytes primed in vitro, a nucleopore culture chamber technique allowing human lymphocytes to be cultured for a period of at least twoweeks has been developed. During the primary culture period in nucleopore chambers, human lymphocytes were sensitized against mitomycin-treated allogenic stimulating cells. It was shown that the stimulated lymphocytes underwent a blastogenic reaction and the results suggest a reversion to the state of small, resting, primed lymphocytes. In vitro primed lymphocytes displayed allogenic memory. This was characteristic of a secondary response, which is shown by the following: 1) acceleration, the peak of thymidine incorporation occuring on day 4, 2) specificity, the accelerated response was observed only when theprimed lymphocytes were confronted with the cell used for priming. Contact with a third party cell did not produce this kind of activation. 3) Amplitude; the peak DNA synthesis response was greater than that of unprimed lymphocytes cultivated for the same length of time." @default.
• W2023385280 created "2016-06-24" @default.
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• W2023385280 date "1975-05-01" @default.
• W2023385280 modified "2023-10-18" @default.
• W2023385280 title "Mixed lymphocyte reactivity of human lymphocytes primedin vitro. I. Secondary response to allogenic lymphocytes" @default.
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• W2023385280 doi "https://doi.org/10.1002/eji.1830050502" @default.
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