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- W2023388788 abstract "Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over 30℃. Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from 30℃ to 37℃. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined." @default.
- W2023388788 created "2016-06-24" @default.
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- W2023388788 date "2010-01-01" @default.
- W2023388788 modified "2023-09-28" @default.
- W2023388788 title "Screening of Cell Cycle-Related Genes ofPleurotus eryngiiUsing Yeast Mutant Strains" @default.
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- W2023388788 doi "https://doi.org/10.4489/myco.2010.38.1.070" @default.
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