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• W2023391073 abstract "Radiolabeled ponasterone A, a high affinity ligand for ecdysteroid receptors which agonises the effects of 20-hydroxyecdysone, was used in combination with thaw-mount autoradiography to study the stage-specific presence of ecdysteroid receptors in the central nervous system of Sarcophaga bullata. In third instar larvae, nuclear high affinity binding of tritiated or iodinated ponasterone A occurs in the same target cells and both radioligands were displaced by an 100-fold excess of unlabeled ponasterone A or an 500-fold excess of 20-hydroxyecdysone. Target neurons for ponasterone A appear first in the third instar larvae on day 4.0 (early wandering stage) where many cells of the perineurium, ring gland, lateral neurosecretory cells in the brain and certain neurons in abdominal ganglia exhibit nuclear high-affinity binding for ponasterone A. At day 5.5 after larviposition, less binding is present in the perineurium but many neurons, including certain neurosecretory cells in the pars intercerebralis, pars lateralis, tritocerebrum, and neurosecretory cell groups 7, 8, 9, and 10 of the dipteran suboesophageal and abdominal ganglia show increased nuclear ecdysteroid binding. At this stage nuclear binding also occurs in the ring gland except in the central corpus allatum and for the first time in the neurons of the inner optic lobes. The results show that ecdysteroid receptors are present in distinct cerebral neurons and that their expression or ecdysteroid-binding capability is under developmental control." @default.
• W2023391073 created "2016-06-24" @default.
• W2023391073 creator A5073532901 @default.
• W2023391073 date "1991-11-01" @default.
• W2023391073 modified "2023-09-24" @default.
• W2023391073 title "Developmental changes in the presence of ecdysteroid receptors in the central nervous system of third instar larvae of Sarcophaga bullata" @default.
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• W2023391073 doi "https://doi.org/10.1016/0165-3806(91)90072-q" @default.
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