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• W2023402503 abstract "We investigated the scoring properties of the mouse formalin test using the time-sampling method recently developed for infant and adult rats. Formalin was injected under the plantar surface of one rear paw (10 μl, 1–8%), and pain behaviours (paw favouring, lifting and licking) and behavioural state were recorded. Correlational and regression analyses indicated that scores composed of combinations of all three pain behaviours, either summed or weighted, provided less variable indices of pain than licking alone. The maximum percent effect (MPE50; i.e. pain behaviour 50% of the time) for the log formalin concentration–effect curves was 3–4% in both phases. Habituation to the test environment prior to testing did not alter the MPE50s, but slopes were lower in unhabituated mice, dramatically increasing the size of the confidence interval. Formalin dose-dependently reduced locomotion, rearing and sniffing in both the first phase and the early part of the second phase. The combination measures were sensitive to morphine (2–8 mg/kg), amphetamine (1–4 mg/kg), dipyrone (50–200 mg/kg), xylazine (0.25–1 mg/kg), and acepromazine (0.25–1 mg/kg), and resistant to diazepam (0.5–2 mg/kg), pimozide (0.05–0.25 mg/kg), pentobarbital (10 and 15 mg/kg) and indomethacin (2–8 mg/kg). Decreased pain was correlated with increased motor activity for morphine and amphetamine, and with decreased activity for xylazine and acepromazine; dipyrone and indomethacin did not alter activity levels." @default.
• W2023402503 created "2016-06-24" @default.
• W2023402503 creator A5040876235 @default.
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• W2023402503 date "2000-12-01" @default.
• W2023402503 modified "2023-10-08" @default.
• W2023402503 title "The formalin test in the mouse: a parametric analysis of scoring properties" @default.
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• W2023402503 doi "https://doi.org/10.1016/s0304-3959(00)00348-1" @default.
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