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- W2023431052 abstract "Abstract It is generally believed that the uptake of sulfobromophthalein by rat liver cells is mediated by a single carrier. Accordingly, kinetic plots obtained using a wide concentration range, failed to give any evidence of heterogeneity in the uptake and gave a K m of 8.9 ± 2.5 μ M and a maximal velocity of 4.2 ± 0.8 nmoles/min/10 6 cells. Na + -taurocholate inhibited competitively, but only partially, sulfobromophthalein uptake by isolated rat liver cells. The taurocholate sensitive component of sulfobromophthalein uptake followed Michaelis Menten kinetics with a K m of 0.9 μ M and a V max of 0.47 nmole/min/10 6 cells. Taurocholate inhibition reveals an heterogeneity in the uptake of sulfobromophthalein not shown in classical kinetic plots, indicating that two carriers are involved in the uptake of this dye by rat liver cells. The taurocholate sensitive carrier which displays an affinity for sulfobromophthalein ten times higher than that of taurocholate insensitive one, is probably identical to the Na + -independent carrier involved in bile salt uptake." @default.
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- W2023431052 date "1981-06-01" @default.
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- W2023431052 title "Two systems are involved in the sulfobromophthalein uptake by rat liver cells: One is shared with bile salts" @default.
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- W2023431052 doi "https://doi.org/10.1016/0006-2952(81)90317-8" @default.
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