FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023441639> ?p ?o ?g. }

• W2023441639 endingPage "2156" @default.
• W2023441639 startingPage "2151" @default.
• W2023441639 abstract "ObjectiveTo study sperm parameters in patients infected with human immunodeficiency virus (HIV)-1 and to analyze mitochondrial DNA (mtDNA) in sperm according to the HIV treatment.DesignObservational study.SettingUniversity-affiliated teaching hospital.Patient(s)Thirty-two patients infected with HIV-1 and 31 noninfected healthy men provided semen samples.Intervention(s)None.Main Outcome Measure(s)After DNA extraction, mtDNA level was assessed using real-time polymerase chain reaction (PCR) (LightCycler) in whole semen and in selected spermatozoa from 90% density centrifugation gradients. For each sample, mtDNA and β-globin gene sequences were amplified and PCR products were quantified. The mtDNA-to-β-globin ratio expressed the number of mtDNA copies per cell.Result(s)Compared with the control group, several sperm parameters were altered in patients with HIV. The number of mtDNA copies per cell in whole semen was increased in HIV-infected patients (6.3 ± 6.3 vs. 3.5 ± 3.2). However, there was no statistically significant difference in mtDNA copy number in the spermatozoa obtained after density gradient centrifugation. The number of nucleoside analogue reverse transcriptase inhibitors (NRTI) taken by patients during treatment significantly influenced the mtDNA level in sperm (1 NRTI 7.6 ± 8.1, 2 NRTIs 7.0 ± 5.1, 3 NRTIs 3.2 ± 2.1).Conclusion(s)Using a specific method to measure sperm mtDNA, we demonstrated a decrease of mtDNA copies in spermatozoa after use of NRTIs with known mitochondrial toxicity. To study sperm parameters in patients infected with human immunodeficiency virus (HIV)-1 and to analyze mitochondrial DNA (mtDNA) in sperm according to the HIV treatment. Observational study. University-affiliated teaching hospital. Thirty-two patients infected with HIV-1 and 31 noninfected healthy men provided semen samples. None. After DNA extraction, mtDNA level was assessed using real-time polymerase chain reaction (PCR) (LightCycler) in whole semen and in selected spermatozoa from 90% density centrifugation gradients. For each sample, mtDNA and β-globin gene sequences were amplified and PCR products were quantified. The mtDNA-to-β-globin ratio expressed the number of mtDNA copies per cell. Compared with the control group, several sperm parameters were altered in patients with HIV. The number of mtDNA copies per cell in whole semen was increased in HIV-infected patients (6.3 ± 6.3 vs. 3.5 ± 3.2). However, there was no statistically significant difference in mtDNA copy number in the spermatozoa obtained after density gradient centrifugation. The number of nucleoside analogue reverse transcriptase inhibitors (NRTI) taken by patients during treatment significantly influenced the mtDNA level in sperm (1 NRTI 7.6 ± 8.1, 2 NRTIs 7.0 ± 5.1, 3 NRTIs 3.2 ± 2.1). Using a specific method to measure sperm mtDNA, we demonstrated a decrease of mtDNA copies in spermatozoa after use of NRTIs with known mitochondrial toxicity." @default.
• W2023441639 created "2016-06-24" @default.
• W2023441639 creator A5016321008 @default.
• W2023441639 creator A5039809724 @default.
• W2023441639 creator A5040159137 @default.
• W2023441639 creator A5043749772 @default.
• W2023441639 creator A5058194563 @default.
• W2023441639 creator A5060353319 @default.
• W2023441639 creator A5088261783 @default.
• W2023441639 creator A5029672173 @default.
• W2023441639 date "2010-11-01" @default.
• W2023441639 modified "2023-10-14" @default.
• W2023441639 title "Decrease of mitochondrial DNA level in sperm from patients infected with human immunodeficiency virus-1 linked to nucleoside analogue reverse transcriptase inhibitors" @default.
• W2023441639 cites W1853388536 @default.
• W2023441639 cites W188968863 @default.
• W2023441639 cites W1979867739 @default.
• W2023441639 cites W1985391395 @default.
• W2023441639 cites W1989206970 @default.
• W2023441639 cites W1989222553 @default.
• W2023441639 cites W1994005638 @default.
• W2023441639 cites W2008681391 @default.
• W2023441639 cites W2010937511 @default.
• W2023441639 cites W2013711685 @default.
• W2023441639 cites W2026364655 @default.
• W2023441639 cites W2031274178 @default.
• W2023441639 cites W2031534477 @default.
• W2023441639 cites W2041841997 @default.
• W2023441639 cites W2057506499 @default.
• W2023441639 cites W2057695604 @default.
• W2023441639 cites W2063184746 @default.
• W2023441639 cites W2072141531 @default.
• W2023441639 cites W2089557992 @default.
• W2023441639 cites W2094527205 @default.
• W2023441639 cites W2106809159 @default.
• W2023441639 cites W2109867862 @default.
• W2023441639 cites W2113716528 @default.
• W2023441639 cites W2121191081 @default.
• W2023441639 cites W2122428976 @default.
• W2023441639 cites W2123398881 @default.
• W2023441639 cites W2140811611 @default.
• W2023441639 cites W2144189605 @default.
• W2023441639 cites W2150867854 @default.
• W2023441639 cites W2159031872 @default.
• W2023441639 cites W2160639559 @default.
• W2023441639 cites W2166870789 @default.
• W2023441639 cites W2288334662 @default.
• W2023441639 cites W2298250249 @default.
• W2023441639 cites W2319337923 @default.
• W2023441639 cites W2320121177 @default.
• W2023441639 cites W2320828273 @default.
• W2023441639 cites W3122481980 @default.
• W2023441639 doi "https://doi.org/10.1016/j.fertnstert.2009.12.080" @default.
• W2023441639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20153854" @default.
• W2023441639 hasPublicationYear "2010" @default.
• W2023441639 type Work @default.
• W2023441639 sameAs 2023441639 @default.
• W2023441639 citedByCount "31" @default.
• W2023441639 countsByYear W20234416392012 @default.
• W2023441639 countsByYear W20234416392013 @default.
• W2023441639 countsByYear W20234416392014 @default.
• W2023441639 countsByYear W20234416392015 @default.
• W2023441639 countsByYear W20234416392016 @default.
• W2023441639 countsByYear W20234416392017 @default.
• W2023441639 countsByYear W20234416392018 @default.
• W2023441639 countsByYear W20234416392019 @default.
• W2023441639 countsByYear W20234416392021 @default.
• W2023441639 countsByYear W20234416392022 @default.
• W2023441639 crossrefType "journal-article" @default.
• W2023441639 hasAuthorship W2023441639A5016321008 @default.
• W2023441639 hasAuthorship W2023441639A5029672173 @default.
• W2023441639 hasAuthorship W2023441639A5039809724 @default.
• W2023441639 hasAuthorship W2023441639A5040159137 @default.
• W2023441639 hasAuthorship W2023441639A5043749772 @default.
• W2023441639 hasAuthorship W2023441639A5058194563 @default.
• W2023441639 hasAuthorship W2023441639A5060353319 @default.
• W2023441639 hasAuthorship W2023441639A5088261783 @default.
• W2023441639 hasBestOaLocation W20234416391 @default.
• W2023441639 hasConcept C104317684 @default.
• W2023441639 hasConcept C153911025 @default.
• W2023441639 hasConcept C156719811 @default.
• W2023441639 hasConcept C159047783 @default.
• W2023441639 hasConcept C16685009 @default.
• W2023441639 hasConcept C24586158 @default.
• W2023441639 hasConcept C2777351918 @default.
• W2023441639 hasConcept C2778093475 @default.
• W2023441639 hasConcept C2781087480 @default.
• W2023441639 hasConcept C28859421 @default.
• W2023441639 hasConcept C49105822 @default.
• W2023441639 hasConcept C54355233 @default.
• W2023441639 hasConcept C552990157 @default.
• W2023441639 hasConcept C71924100 @default.
• W2023441639 hasConcept C86803240 @default.
• W2023441639 hasConceptScore W2023441639C104317684 @default.
• W2023441639 hasConceptScore W2023441639C153911025 @default.
• W2023441639 hasConceptScore W2023441639C156719811 @default.
• W2023441639 hasConceptScore W2023441639C159047783 @default.
• W2023441639 hasConceptScore W2023441639C16685009 @default.
• W2023441639 hasConceptScore W2023441639C24586158 @default.

• next