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• W2023441751 abstract "The ‘suspension method’ for detection of micronuclei in early spermatids (Golgi phase 1 + 2) of rats was used to study induction of chromosomal damage in spermatocytes exposed to single (50 and 100 mg/kg) or fractionated (4 × 50 mg/kg; 24-h intervals) doses of acrylamide. Animals were killed at different time intervals after treatment to measure induction of damage in different stages of spermatocyte development. A statisticallyl significant enhancement of micronucleus frequencies was found after exposure of pre-leptotene spermactocytes to a single dose of 100 mg/kg (days 18 and 20 after treatment) or a fractionated dose of 4 × 50 mg/kg (day 19). In the latter case there was also a significant effect in zygotene spermatocytes that were sampled 15 days after treatment. Comparative studies indicated that the original ‘suspension method’ can be simplified by omitting enzyme treatments for the release of germ cells from the seminiferous tubules. In the present acrylamide study the old and new procedures gave similar results." @default.
• W2023441751 created "2016-06-24" @default.
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• W2023441751 date "1994-09-01" @default.
• W2023441751 modified "2023-10-12" @default.
• W2023441751 title "Increased frequencies of micronuclei in early spermatids of rats following exposure of young primary spermatocytes to acrylamide" @default.
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• W2023441751 doi "https://doi.org/10.1016/0027-5107(94)90099-x" @default.
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