FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023446056> ?p ?o ?g. }

• W2023446056 endingPage "733" @default.
• W2023446056 startingPage "726" @default.
• W2023446056 abstract "It has been recently claimed that polymorphism for the vitamin D receptor (VDR) influences several aspects of calcium and bone metabolism. To evaluate the physiologic plausibility of these claims, we compared the abundance of the VDR mRNA in peripheral blood mononuclear cells (PBMCs) between different VDR genotypes using a quantitative reverse transcribed polymerase chain reaction-based method. The method is based on the coamplification of VDR cDNA and an internal standard consisting of known concentrations of a human VDR CDNA mutated at a BglII restriction site; the interassay coefficient of variation is 11%. To validate the method, we made use of earlier receptor binding studies indicating that normal human monocytes and activated, but not resting, lymphocytes expressed the VDR. The concentration of the VDR mRNA was 10(-8) to 10(-7) g/g of total RNA in cell-sorted monocytes and in in vitro activated lymphocytes, but only 10(-12) g/g of total mRNA in resting lymphocytes, establishing that the VDR mRNA determined by our method in PBMCs is due to constitutive expression in monocytes. Following an initial genotype screening of 85 normal volunteers by polymerase chain reaction or restriction fragment length polymorphism analysis, 14 individuals with the Bb genotype, 12 with the bb genotype, and 12 with the BB genotype were selected. The concentration of the VDR mRNA, corrected for the number of monocytes, was similar among the three genotype groups, as were the other variables examined: serum calcitriol, serum osteocalcin, and vertebral and hip bone density. We conclude that VDR polymorphism does not affect the abundance of the VDR mRNA." @default.
• W2023446056 created "2016-06-24" @default.
• W2023446056 creator A5011446671 @default.
• W2023446056 creator A5012483707 @default.
• W2023446056 creator A5019660823 @default.
• W2023446056 creator A5021379552 @default.
• W2023446056 creator A5027634434 @default.
• W2023446056 creator A5040820750 @default.
• W2023446056 creator A5054118361 @default.
• W2023446056 creator A5074374428 @default.
• W2023446056 creator A5076606940 @default.
• W2023446056 creator A5087652874 @default.
• W2023446056 date "1997-05-01" @default.
• W2023446056 modified "2023-10-09" @default.
• W2023446056 title "Quantification of Vitamin D Receptor mRNA by Competitive Polymerase Chain Reaction in PBMC: Lack of Correspondence with Common Allelic Variants" @default.
• W2023446056 cites W1976640699 @default.
• W2023446056 cites W1978802063 @default.
• W2023446056 cites W2006949906 @default.
• W2023446056 cites W2007273969 @default.
• W2023446056 cites W2007331929 @default.
• W2023446056 cites W2009180025 @default.
• W2023446056 cites W2034725130 @default.
• W2023446056 cites W2040799254 @default.
• W2023446056 cites W2041709092 @default.
• W2023446056 cites W2057392561 @default.
• W2023446056 cites W2088378090 @default.
• W2023446056 cites W2100156983 @default.
• W2023446056 cites W2117263637 @default.
• W2023446056 cites W2118333888 @default.
• W2023446056 cites W280121275 @default.
• W2023446056 cites W4249223714 @default.
• W2023446056 cites W4250438352 @default.
• W2023446056 cites W4256306848 @default.
• W2023446056 doi "https://doi.org/10.1359/jbmr.1997.12.5.726" @default.
• W2023446056 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9144338" @default.
• W2023446056 hasPublicationYear "1997" @default.
• W2023446056 type Work @default.
• W2023446056 sameAs 2023446056 @default.
• W2023446056 citedByCount "90" @default.
• W2023446056 countsByYear W20234460562012 @default.
• W2023446056 countsByYear W20234460562014 @default.
• W2023446056 countsByYear W20234460562015 @default.
• W2023446056 countsByYear W20234460562016 @default.
• W2023446056 countsByYear W20234460562017 @default.
• W2023446056 countsByYear W20234460562018 @default.
• W2023446056 countsByYear W20234460562019 @default.
• W2023446056 crossrefType "journal-article" @default.
• W2023446056 hasAuthorship W2023446056A5011446671 @default.
• W2023446056 hasAuthorship W2023446056A5012483707 @default.
• W2023446056 hasAuthorship W2023446056A5019660823 @default.
• W2023446056 hasAuthorship W2023446056A5021379552 @default.
• W2023446056 hasAuthorship W2023446056A5027634434 @default.
• W2023446056 hasAuthorship W2023446056A5040820750 @default.
• W2023446056 hasAuthorship W2023446056A5054118361 @default.
• W2023446056 hasAuthorship W2023446056A5074374428 @default.
• W2023446056 hasAuthorship W2023446056A5076606940 @default.
• W2023446056 hasAuthorship W2023446056A5087652874 @default.
• W2023446056 hasBestOaLocation W20234460561 @default.
• W2023446056 hasConcept C104317684 @default.
• W2023446056 hasConcept C105580179 @default.
• W2023446056 hasConcept C124490489 @default.
• W2023446056 hasConcept C126322002 @default.
• W2023446056 hasConcept C134018914 @default.
• W2023446056 hasConcept C135763542 @default.
• W2023446056 hasConcept C137061746 @default.
• W2023446056 hasConcept C153639570 @default.
• W2023446056 hasConcept C153911025 @default.
• W2023446056 hasConcept C168007829 @default.
• W2023446056 hasConcept C170493617 @default.
• W2023446056 hasConcept C179639408 @default.
• W2023446056 hasConcept C180754005 @default.
• W2023446056 hasConcept C202751555 @default.
• W2023446056 hasConcept C2776079296 @default.
• W2023446056 hasConcept C49105822 @default.
• W2023446056 hasConcept C54355233 @default.
• W2023446056 hasConcept C71924100 @default.
• W2023446056 hasConcept C86803240 @default.
• W2023446056 hasConceptScore W2023446056C104317684 @default.
• W2023446056 hasConceptScore W2023446056C105580179 @default.
• W2023446056 hasConceptScore W2023446056C124490489 @default.
• W2023446056 hasConceptScore W2023446056C126322002 @default.
• W2023446056 hasConceptScore W2023446056C134018914 @default.
• W2023446056 hasConceptScore W2023446056C135763542 @default.
• W2023446056 hasConceptScore W2023446056C137061746 @default.
• W2023446056 hasConceptScore W2023446056C153639570 @default.
• W2023446056 hasConceptScore W2023446056C153911025 @default.
• W2023446056 hasConceptScore W2023446056C168007829 @default.
• W2023446056 hasConceptScore W2023446056C170493617 @default.
• W2023446056 hasConceptScore W2023446056C179639408 @default.
• W2023446056 hasConceptScore W2023446056C180754005 @default.
• W2023446056 hasConceptScore W2023446056C202751555 @default.
• W2023446056 hasConceptScore W2023446056C2776079296 @default.
• W2023446056 hasConceptScore W2023446056C49105822 @default.
• W2023446056 hasConceptScore W2023446056C54355233 @default.
• W2023446056 hasConceptScore W2023446056C71924100 @default.
• W2023446056 hasConceptScore W2023446056C86803240 @default.
• W2023446056 hasIssue "5" @default.
• W2023446056 hasLocation W20234460561 @default.

• next