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- W2023448082 abstract "A simple and convenient method for the cryopreservation of sea cucumberApostichopus japonicus (Selenka) sperm was tested in the present study. The highest motility (76.7 2.9%) of post-thawing sperm was obtained in15% dimethyl sulphoxide (DMSO) with a1:9 dilution (semen volume to DMSO volume) when 0.5 mL semen^DMSO mixture was frozen at 6 cm above liquid N2 in a closed styrofoam box. After thawing, sperm cryopreserved in glycerol almost lost motility entirely. Although there was no signi¢cant diierence in percentage of motile sperm between 15% and 20% DMSO, the duration of sperm motility of 15% DMSO group was longer than that of 20% DMSO group. The motility of post-thawing sperm enhanced when the dilution ratio of semen increased from 1:1 to 1:9. Morphological changes such as the loss of mitochondria, swollen plasma membrane and broken or rolled-up tails were observed in post-thawing sperm using an eosin^nigrosin staining. The fertility of cryopreserved sperm was signi¢cantly lower than that of unfrozen sperm. The 10-fold increase in sperm to egg ratio resulted in double fertility for cryopreserved sperm, and about 70% fertility relative to the control." @default.
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- W2023448082 date "2006-10-01" @default.
- W2023448082 modified "2023-09-25" @default.
- W2023448082 title "Cryopreservation of sea cucumber Apostichopus japonicus (Selenka) sperm" @default.
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- W2023448082 doi "https://doi.org/10.1111/j.1365-2109.2006.01581.x" @default.
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