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- W2023474703 abstract "The Walker Carcinosarcoma-256 was preserved for 404 days at liquid nitrogen temperature. The cytological analysis of frozen cells was performed sequentially. The modal chromosome number was constantly 57 in each frozen generation. The karyotype constitutions were also stable throughout the experiments. The 6 marker chromosomes, i.e., largest submetacentric, large metacentric, large submetacentric, medium-sized metacentric, medium-sized submetacentric, and small satellited telocentric were always confirmed in each of the frozen cells analyzed. No differences existed in the results of the chromosomal observations of the frozen cells and the stock cells of the continuous animal-passaged line. The frozen-thawed specimens of cell suspension resulted in as high a percentage of successful transplants into rats as small pieces of tumor tissue. It was clearly demonstrated that tumor cells of solid Walker rat tumor, when placed in suspension, can be stored at liquid nitrogen temperatures for a year or more without chromosomal abnormality." @default.
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- W2023474703 date "1974-12-01" @default.
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- W2023474703 title "Sequential chromosome analysis of Walker Carcinosarcoma-256 implanted into rats after frozen storage" @default.
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- W2023474703 doi "https://doi.org/10.1016/0011-2240(74)90119-9" @default.
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