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• W2023479062 abstract "A minizyme is a hammerhead ribozyme with short oligonucleotide linkers instead of stem/loop II. In a previous study we demonstrated that a minizyme with high-level activity forms a dimeric structure with a common stem II (Amontov and Taira, J. Am. Chem. Soc., 118 (1996) 1624-1628). As a continuation of this study, we recently examined whether a short oligonucleotide linker at stem/loop II could be replaced by a triterpenoid linker and whether the resulting minizymes with bulky hydrophobic groups would form dimeric structures. In contrast to the minizyme with high-level activity that we characterized in the previous study, minizymes that contained a triterpenoid group had low cleavage activities. The nature of the dependence of the activity on the concentration of ribozyme revealed that these minizymes with a triterpenoid group do not form dimeric structures. Thus, the low activities of these structures can be attributed to their failure to form dimers." @default.
• W2023479062 created "2016-06-24" @default.
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• W2023479062 date "1996-09-02" @default.
• W2023479062 modified "2023-10-16" @default.
• W2023479062 title "Catalytic activities of hammerhead ribozymes with a triterpenoid linker instead of stem/loop II" @default.
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• W2023479062 doi "https://doi.org/10.1016/0014-5793(96)00814-9" @default.
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