Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023492746> ?p ?o ?g. }
- W2023492746 endingPage "13462" @default.
- W2023492746 startingPage "13449" @default.
- W2023492746 abstract "The nonstructural 5A (NS5A) protein of the hepatitis C virus (HCV) is a multifunctional protein that is implicated in viral replication and pathogenesis. We report here that NS5A of HCV-1a is cleaved at multiple sites by caspase proteases in transfected cells. Two cleavage sites at positions Asp154 and 248DXXD251 were mapped. Cleavage at Asp154 has been previously recognized as one of the caspase cleavage sites for the NS5A protein of HCV genotype 1b (1, 2) and results in the production of a 17-kDa fragment. The sequence 248DXXD251 is a novel caspase recognition motif for NS5A and is responsible for the production of a 31-kDa fragment. Furthermore, we show that Arg217 is implicated in the production of the previously described 24-kDa product, whose accumulation is affected by both calpain and caspase inhibitors. We also showed that caspase-mediated cleavage occurs in the absence of exogenous proapoptotic stimuli and is not related to the accumulation of the protein in the endoplasmic reticulum. Interestingly, our data indicate that NS5A is targeted by at least two different caspases and suggest that caspase 6 is implicated in the production of the 17-kDa fragment. Most importantly, we report that, all the detectable NS5A fragments following caspase-mediated cleavage are C-terminal-truncated forms of NS5A and are mainly localized in the cytosol. Thus, in sharp contrast to the current view we found no evidence supporting a role for caspase-mediated cleavage in the transport of the NS5A protein to the nucleus, which could lead to transcriptional activation." @default.
- W2023492746 created "2016-06-24" @default.
- W2023492746 creator A5016951659 @default.
- W2023492746 creator A5053720647 @default.
- W2023492746 creator A5059174311 @default.
- W2023492746 date "2006-05-01" @default.
- W2023492746 modified "2023-09-27" @default.
- W2023492746 title "The NS5A Protein of the Hepatitis C Virus Genotype 1a Is Cleaved by Caspases to Produce C-terminal-truncated Forms of the Protein That Reside Mainly in the Cytosol" @default.
- W2023492746 cites W1942214720 @default.
- W2023492746 cites W1956249391 @default.
- W2023492746 cites W1964008079 @default.
- W2023492746 cites W1967912613 @default.
- W2023492746 cites W1968426124 @default.
- W2023492746 cites W1969728880 @default.
- W2023492746 cites W1969781490 @default.
- W2023492746 cites W1973165481 @default.
- W2023492746 cites W1974865581 @default.
- W2023492746 cites W1975273858 @default.
- W2023492746 cites W1975922979 @default.
- W2023492746 cites W1976271632 @default.
- W2023492746 cites W1977990503 @default.
- W2023492746 cites W1978033703 @default.
- W2023492746 cites W1981106016 @default.
- W2023492746 cites W1981845120 @default.
- W2023492746 cites W1982303529 @default.
- W2023492746 cites W1984338631 @default.
- W2023492746 cites W1986178002 @default.
- W2023492746 cites W1986348016 @default.
- W2023492746 cites W1989636817 @default.
- W2023492746 cites W1990250019 @default.
- W2023492746 cites W1994750040 @default.
- W2023492746 cites W1995539563 @default.
- W2023492746 cites W1997307715 @default.
- W2023492746 cites W2001030705 @default.
- W2023492746 cites W2004688340 @default.
- W2023492746 cites W2005228651 @default.
- W2023492746 cites W2005310997 @default.
- W2023492746 cites W2006309458 @default.
- W2023492746 cites W2007939817 @default.
- W2023492746 cites W2009354010 @default.
- W2023492746 cites W2010832638 @default.
- W2023492746 cites W2012333728 @default.
- W2023492746 cites W2013100750 @default.
- W2023492746 cites W2013468140 @default.
- W2023492746 cites W2015965270 @default.
- W2023492746 cites W2016811060 @default.
- W2023492746 cites W2022568067 @default.
- W2023492746 cites W2023437754 @default.
- W2023492746 cites W2024086482 @default.
- W2023492746 cites W2024548275 @default.
- W2023492746 cites W2026151480 @default.
- W2023492746 cites W2029272473 @default.
- W2023492746 cites W2031770118 @default.
- W2023492746 cites W2033686860 @default.
- W2023492746 cites W2034213169 @default.
- W2023492746 cites W2034915619 @default.
- W2023492746 cites W2039422277 @default.
- W2023492746 cites W2043585116 @default.
- W2023492746 cites W2043690695 @default.
- W2023492746 cites W2044973532 @default.
- W2023492746 cites W2048502224 @default.
- W2023492746 cites W2050383215 @default.
- W2023492746 cites W2051658957 @default.
- W2023492746 cites W2051756088 @default.
- W2023492746 cites W2058159821 @default.
- W2023492746 cites W2059145805 @default.
- W2023492746 cites W2059280966 @default.
- W2023492746 cites W2059530064 @default.
- W2023492746 cites W2062894160 @default.
- W2023492746 cites W2064766502 @default.
- W2023492746 cites W2067015560 @default.
- W2023492746 cites W2067985632 @default.
- W2023492746 cites W2068798911 @default.
- W2023492746 cites W2069059422 @default.
- W2023492746 cites W2069531923 @default.
- W2023492746 cites W2073578569 @default.
- W2023492746 cites W2077230125 @default.
- W2023492746 cites W2078887917 @default.
- W2023492746 cites W2082933842 @default.
- W2023492746 cites W2083282915 @default.
- W2023492746 cites W2083573042 @default.
- W2023492746 cites W2084019205 @default.
- W2023492746 cites W2085158671 @default.
- W2023492746 cites W2089829321 @default.
- W2023492746 cites W2090825777 @default.
- W2023492746 cites W2091619297 @default.
- W2023492746 cites W2092670341 @default.
- W2023492746 cites W2095199759 @default.
- W2023492746 cites W2095786152 @default.
- W2023492746 cites W2098368373 @default.
- W2023492746 cites W2103187359 @default.
- W2023492746 cites W2103272101 @default.
- W2023492746 cites W2106132583 @default.
- W2023492746 cites W2107983204 @default.
- W2023492746 cites W2110289105 @default.
- W2023492746 cites W2110850519 @default.
- W2023492746 cites W2114915924 @default.
- W2023492746 cites W2115610036 @default.