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- W2023494064 abstract "Cell culture and growth in space is crucial to understand the cellular responses under microgravity. The effects of microgravity were coupled with such environment restrictions as medium perfusion, in which the underlying mechanism has been poorly understood. In the present work, a customer-made counter sheet-flow sandwich cell culture device was developed upon a biomechanical concept from fish gill breathing. The sandwich culture unit consists of two side chambers where the medium flow is counter-directional, a central chamber where the cells are cultured, and two porous polycarbonate membranes between side and central chambers. Flow dynamics analysis revealed the symmetrical velocity profile and uniform low shear rate distribution of flowing medium inside the central culture chamber, which promotes sufficient mass transport and nutrient supply for mammalian cell growth. An on-orbit experiment performed on a recovery satellite was used to validate the availability of the device." @default.
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- W2023494064 date "2008-04-16" @default.
- W2023494064 modified "2023-09-26" @default.
- W2023494064 title "A Novel Counter Sheet-flow Sandwich Cell Culture Device for Mammalian Cell Growth in Space" @default.
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- W2023494064 doi "https://doi.org/10.1007/s12217-008-9008-5" @default.
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